《Interference from high-dose biotin intake in immunoassays for potentially time-critical analytes by Roche. Evaluation of a countermeasure for worst-case scenarios》 was published in Archives of Pathology & Laboratory Medicine in 2020. These research results belong to Mrosewski, Ingo; Urbank, Matthias; Stauch, Thomas; Switkowski, Rafael. Category: imidazoles-derivatives The article mentions the following:
Context.-Immunoassays using the interaction between streptavidin and biotin are used for clin. chem. analytes on platforms by many different manufacturers. The design can be susceptible to interference from high-dose biotin intake in patients, which remains an often-overlooked confounder despite recently increased awareness. Objective.-To evaluate an easily implementable method of in vitro biotin depletion for the removal of biotin interference in immunoassays for potentially time-critical analytes. Design.-A biotin stock solution was made and deidentified patient samples were spiked to reach a biotin concentration of 1.126 x 106 pg/mL, the maximum reported biotin concentration 1 to 2 h after a single oral dose of 300 mg biotin. Then, the resulting interference in Elecsys immunoassays for cortisol, cyclosporine A, tacrolimus, digitoxin, TSH, free triiodothyronine, free thyroxine, C-peptide, insulin, Nterminal pro-β-type natriuretic peptide, troponin T high sensitive, human immunodeficiency virus, procalcitonin, β human chorionic gonadotropin, toxoplasma IgM, and toxoplasma IgG was evaluated before and after biotin depletion using streptavidin particles. Results.-All tested immunoassays, with the exception of toxoplasma IgM and toxoplasma IgG, suffered from significant biotin interference. The depletion protocol removed assay interference due to biotin and produced results that were close or identical to initial prespike measurements. Conclusions.-Despite an increase in turnaround times, biotin adsorption is a feasible countermeasure for biotin interference in Elecsys immunoassays. Until test kits with an increased resistance to the interference from high-dose biotin intake are distributed, the evaluated protocol can provide results properly reflecting the patient’s clin. condition. In addition to this study using 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid, there are many other studies that have used 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5Category: imidazoles-derivatives) was used in this study.
5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.Category: imidazoles-derivatives The biotin/avidin or biotin/streptavidin interaction is utilized in many labeling and purification schemes.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem