Shi, Fei; Deng, Zheng; Zhou, Zheng; Jiang, Bo; Jiang, Chen-Yi; Zhao, Rui-Zhe; Sun, Feng; Cui, Di; Sun, Meng-Hao; Sun, Qian; Wang, Xing-Jie; Wu, Qi; Xia, Shu-Jie; Han, Bang-Min published the artcile< Heat injured stromal cells-derived exosomal EGFR enhances prostatic wound healing after thulium laser resection through EMT and NF-κB signaling>, Computed Properties of 6823-69-4, the main research area is thulium nuclearfactor Epithelial mesenchymal transition epidermalgrowth factorreceptor stromalcell woundhealing; benign prostatic hyperplasia (BPH); epidermal growth factor receptor (EGFR); exosome; nuclear factor-kappa B (NF-κB); shallow heat injury; wound healing.
This study investigated shallow heat injury to prostate stromal fibroblasts and epithelial cells and their interaction to regulate the wound healing and the underlying mol. events. Prostate stromal fibroblasts and epithelial cells were cultured individually or cocultured and subjected to shallow heat injury for assessments of cell proliferation, migration, apoptosis, cell cycle distribution, and gene expression. The supernatant of heat-injured WPMY-1 cells was collected for exosome extraction and assessments. Furthermore, beagle dogs received thulium laser resection of the prostate (TmLRP) and randomly divided into Gefitinib, GW4869, and control treatment for the histol. anal., tissue re-epithelialization, and epidermal growth factor receptor (EGFR) expression on the prostatic wound surface. Immunofluorescence was to evaluate p63-pos. basal progenitor cell trans-differentiation and macrophage polarization and ELISA was to detect cytokine levels in beagles’ urine. Shallow heat injury caused these cells to enter a stressed state and enhanced their crosstalk. The prostate stromal fibroblasts produced and secreted more exosomal-EGFR and other cytokines and chemokines after shallow heat injury, resulting in increased proliferation and migration of prostate epithelial cells during wound healing. The wound healing of the canine prostatic urethra following the TmLRP procedure was slower in the Gefitinib and GW4869 treatment group than in the control group of animals. Immunofluorescence and ELISA showed that reduced EGFR expression interrupted macrophage polarization but increased the inflammatory response. Shallow heat injury was able to promote the interaction of prostate stromal cells with prostate epithelial cells to enhance wound healing. Stromal-derived exosomal-EGFR plays a crucial role in the balance of the macrophage polarization and prostatic wound healing.
Prostate (Hoboken, NJ, United States) published new progress about Apoptosis. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Computed Properties of 6823-69-4.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem