Matsuki-Fukushima, Miwako; Fujikawa, Kaoru; Inoue, Satoshi; Nakamura, Masanori published the artcile< Expression and localization of CD63 in the intracellular vesicles of odontoblasts>, SDS of cas: 6823-69-4, the main research area is expression intracellular vesicle odontoblast; CD63; Dentinogenesis; Odontoblast; Tooth development; Vesicle transport.
We hypothesized that odontoblasts release exosomes as well as dental pulp cells and focused on the exosome membrane marker CD63. Odontoblasts are well-differentiated mesenchymal cells that produce dentin. Dental pulp, a tissue complex formed with odontoblasts, releases exosomes to epithelial cells and stimulates their differentiation to ameloblasts. However, the localization of CD63 in differentiated odontoblasts is poorly understood. Therefore, herein, we aimed to reveal the expression of CD63 in odontoblasts during tooth development. We first investigated the localization of CD63 in mouse incisors and molars using immunofluorescence. In adult mouse incisors, the anti-CD63 antibody was pos. in mature odontoblasts and dental pulp cells but not in pre-odontoblasts along the ameloblasts in the apical bud. Addnl., the anti-CD63 antibody was observed as a vesicular shape in the apical area of odontoblast cytosol and inside Tomes’ fibers. The anti-CD63 antibody-pos. vesicles were also observed using immunoelectron microscopy. Moreover, during mouse mandibular molar tooth morphogenesis (E16 to postnatal 6 wk), labeling of anti-CD63 antibody was pos. in the odontoblasts at E18. In contrast, the anti-CD63 antibody was pos. in the dental pulp after postnatal day 10. Furthermore, anti-CD63 antibody was merged with the multivesicular body marker Rab7 in dental pulp tissues but not with the lysosome marker Lamp1. Finally, we determined the effect of a ceramide-generation inhibitor GW4869 on the mouse organ culture of tooth germ in vitro. After 28 days of GW4869 treatment, both CD63 and Rab7 were neg. in Tomes’ fibers, but were pos. in control odontoblasts. These results suggest that CD63-pos. vesicular organelles are important for mouse tooth morphogenesis.
Histochemistry and Cell Biology published new progress about Adult, mammalian. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, SDS of cas: 6823-69-4.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem