Okuro, Renata Tiemi’s team published research in Pulmonary Pharmacology & Therapeutics in 2018-06-30 | 6823-69-4

Pulmonary Pharmacology & Therapeutics published new progress about Alveolus. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Category: imidazoles-derivatives.

Okuro, Renata Tiemi; Machado, Mariana Nascimento; Casquilho, Natalia Vasconcelos; Jardim-Neto, Alcendino; Roncally-Carvalho, Alysson; Atella, Georgia Correa; Zin, Walter Araujo published the artcile< The role of sphingolipid metabolism disruption on lipopolysaccharide-induced lung injury in mice>, Category: imidazoles-derivatives, the main research area is lung injury sphingolipid metabolism disruption; Ceramide; Enzyme inhibitors; Inflammation; Lipopolysaccharide; Lung injury; Sphingolipid.

This study assessed pulmonary outcomes generated by inhibiting key enzymes of sphingolipid metabolism pathways related to ceramide synthesis in a murine model of lung injury induced by lipopolysaccharide (LPS). C57BL/6 male adult mice received LPS intratracheally and the expressions of acid sphingomyelinase (ASM), neutral sphingomyelinase (NSM), serine palmitoyl transferase (SPT) and dihydroceramide synthase (DS) were assessed at 2, 4, 6, 12 and 24 h after LPS instillation in lung homogenate (n = 30). The pharmacol. inhibition of ASM, NSM, SPT and DS were assayed in other mice groups by three different doses of desipramine, GW4869, myriocin and fumonisin, resp. (n = 90). Their most EDs were administered i.p. 1 or 2 h before LPS to different animal groups (n = 120). Mice underwent determination of pulmonary mechanics, lung histopathol. aspects and apoptosis. The expression levels of the enzymes reached their peak at 2-4 h after LPS administration. ASM inhibition attenuated alveolar collapse and GW4869 decreased lung elastance, proinflammatory cytokines’ levels and was more effective to improve alveolar collapse than desipramine. On the other hand, SPT blockage aggravated lung lesion and no effects it was observed with fumonisin. Moreover, simultaneous administration of inhibitors (desipramine + GW4869, myriocin + fumonisin and all inhibitors together) resulted in no changes. Blockage of sphingomyelinases and the de novo pathways improved and aggravated lung injury, resp., putatively suggesting specific targets to therapeutic strategies in LPS-induced lung injury.

Pulmonary Pharmacology & Therapeutics published new progress about Alveolus. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Category: imidazoles-derivatives.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem