Rong, Yuluo; Ji, Chengyue; Wang, Zhuanghui; Ge, Xuhui; Wang, Jiaxing; Ye, Wu; Tang, Pengyu; Jiang, Dongdong; Fan, Jin; Yin, Guoyong; Liu, Wei; Cai, Weihua published the artcile< Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury>, Safety of p-Benzenediacrylanilide, 4′,4′′-di-2-imidazolin-2-yl-, dihydrochloride, the main research area is traumatic spinal cord injury CCL2 astrocyte microglia neuronal apoptosis; Astrocyte; CCL2; Microglia; Neuron; Small extracellular vesicles; Spinal cord injury.
Spinal cord injury (SCI) is a severe traumatic disease which causes high disability and mortality rates. The mol. pathol. features after spinal cord injury mainly involve the inflammatory response, microglial and neuronal apoptosis, abnormal proliferation of astrocytes, and the formation of glial scars. However, the microenvironmental changes after spinal cord injury are complex, and the interactions between glial cells and nerve cells remain unclear. Small extracellular vesicles (sEVs) may play a key role in cell communication by transporting RNA, proteins, and bioactive lipids between cells. Few studies have examined the intercellular communication of astrocytes through sEVs after SCI. The inflammatory signal released from astrocytes is known to initiate microglial activation, but its effects on neurons after SCI remain to be further clarified. Electron microscopy (TEM), nanoparticle tracking anal. (NTA), and western blotting were applied to characterize sEVs. We examined microglial activation and neuronal apoptosis mediated by astrocyte activation in an exptl. model of acute spinal cord injury and in cell culture in vitro. Our results indicated that astrocytes activated after spinal cord injury release CCL2, act on microglia and neuronal cells through the sEV pathway, and promote neuronal apoptosis and microglial activation after binding the CCR2. Subsequently, the activated microglia release IL-1β, which acts on neuronal cells, thereby further aggravating their apoptosis. This study elucidates that astrocytes interact with microglia and neurons through the sEV pathway after SCI, enriching the mechanism of CCL2 in neuroinflammation and spinal neurodegeneration, and providing a new theor. basis of CCL2 as a therapeutic target for SCI.
Journal of Neuroinflammation published new progress about Apoptosis. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Safety of p-Benzenediacrylanilide, 4′,4′′-di-2-imidazolin-2-yl-, dihydrochloride.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem