Cui, Yigong; Fu, Shenglong; Sun, Dong; Xing, Junchao; Hou, Tianyong; Wu, Xuehui published the artcile< EPC-derived exosomes promote osteoclastogenesis through LncRNA-MALAT1>, Application of C30H30Cl2N6O2, the main research area is endothelial progenitor cell exosome; bone marrow derived macrophage osteoclastogenesis MALAT1; ITGB1; LncRNA-MALAT1; bone marrow-derived macrophages; bone repair; endothelial progenitor cells; miR-124; osteoclastogenesis.
Bone repair involves bone resorption through osteoclastogenesis and the stimulation of neovascularization and osteogenesis by endothelial progenitor cells (EPCs). However, the role of EPCs in osteoclastogenesis is unclear. In this study, we assess the effects of EPC-derived exosomes on the migration and osteoclastic differentiation of primary mouse bone marrow-derived macrophages (BMMs) in vitro using immunofluorescence, western blotting, RT-PCR and Transwell assays. We also evaluated the effects of EPC-derived exosomes on the homing and osteoclastic differentiation of transplanted BMMs in a mouse bone fracture model in vivo. We found that EPCs cultured with BMMs secreted exosomes into the medium and, compared with EPCs, exosomes had a higher expression level of LncRNA-MALAT1. We confirmed that LncRNA-MALAT1 directly binds to miR-124 to neg. control miR124 activity. Moreover, overexpression of miR-124 could reverse the migration and osteoclastic differentiation of BMMs induced by EPC-derived exosomes. A dual-luciferase reporter assay indicated that the integrin ITGB1 is the target of miR-124. Mice treated with EPC-derived exosome-BMM co-transplantations exhibited increased neovascularization at the fracture site and enhanced fracture healing compared with those treated with BMMs alone. Overall, our results suggest that EPC-derived exosomes can promote bone repair by enhancing recruitment and differentiation of osteoclast precursors through LncRNA-MALAT1.
Journal of Cellular and Molecular Medicine published new progress about Argonaute proteins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Application of C30H30Cl2N6O2.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem