《A novel 2-aminobenzimidazole-based compound Jzu 17 exhibits anti-angiogenesis effects by targeting VEGFR-2 signalling》 was written by Lien, Jin-Cherng; Chung, Chi-Li; Huang, Tur-Fu; Chang, Tsung-Chia; Chen, Kuan-Chung; Gao, Ging-Yan; Hsu, Ming-Jen; Huang, Shiu-Wen. Reference of 1H-Benzo[d]imidazol-2-amineThis research focused onumbilical vein endothelial cell 2 aminobenzimidazole Jzu 17 antiangiogenesis. The article conveys some information:
In this study, we aim to characterize the anti-angiogenic mechanisms of a novel 2-aminobenzimidazole-based compound, Jzu 17, in an effort to develop novel angiogenesis inhibitor. Exptl. Approach : Effects of Jzu 17 on endothelial cell proliferation, migration, invasion, and activation of signalling mols. induced by VEGF-A, were analyzed by immunoblotting, MTT, BrdU, migration, and invasion assays. We performed tube formation assay, aorta ring sprouting assay, matrigel plug assay, and a mouse model of metastasis to evaluate ex vivo and in vivo anti-angiogenic effects of Jzu 17. Key Results : Jzu 17 inhibited VEGF-A-induced cell proliferation, migration, invasion, and endothelial tube formation of HUVECs. Jzu 17 suppressed VEGF-A-induced microvessel sprouting ex vivo and attenuated VEGF-A- or tumor cell-induced neovascularization in vivo. Jzu 17 also reduced B16F10 melanoma lung metastasis. In addition, Jzu 17 inhibited the phosphorylation of VEGFR-2 and its downstream signalling mols. in VEGF-A-stimulated HUVECs. Results from computer modeling further showed that Jzu 17 binds to VEGFR-2 with high affinity. Conclusions and Implications : Jzu 17 may inhibit endothelial remodelling and suppress angiogenesis through targeting VEGF-A-VEGFR-2 signalling. These results also suggest Jzu 17 as a potential lead compound and warrant the clin. development of similar agents in the treatment of cancer and angiogenesis-related diseases.1H-Benzo[d]imidazol-2-amine(cas: 934-32-7Reference of 1H-Benzo[d]imidazol-2-amine) was used in this study.
1H-Benzo[d]imidazol-2-amine(cas: 934-32-7) can be used in the hydrolysis of a choline carbonate. It was also used in the synthesis of imidazo[1,2-a]benzimidazoles.Reference of 1H-Benzo[d]imidazol-2-amine
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem