Month: October 2022

《Thiol-Cleavable Biotin for Chemical and Enzymatic Biotinylation and Its Application to Mitochondrial TurboID Proteomics》 was written by Li, Haorong; Frankenfield, Ashley M.; Houston, Ryan; Sekine, Shiori; Hao, Ling. COA of Formula: C10H16N2O3SThis research focused onTurboID; cleavable biotin; mitochondrion; proximity labeling; streptavidin. The article conveys some information:

Protein biotinylation via chem. or enzymic reactions is often coupled with streptavidin-based enrichment and on-bead digestion in numerous biol. applications. However, the popular on-bead digestion method faces major challenges of streptavidin contamination, overwhelming signals from endogenous biotinylated proteins, the lost information on biotinylation sites, and limited sequence coverage of enriched proteins. Here, we explored thiol-cleavable biotin as an alternative approach to elute biotinylated proteins from streptavidin-coated beads for both chem. biotinylation and biotin ligase-based proximity labeling. All possible amino acid sites for biotinylation were thoroughly evaluated in addition to the primary lysine residue. We found that biotinylation at lysine residues notably reduces the trypsin digestion efficiency, which can be mitigated by the thiol-cleavable biotinylation method. We then evaluated the applicability of thiol-cleavable biotin as a substrate for proximity labeling in living cells, where TurboID biotin ligase was engineered onto the mitochondrial inner membrane facing the mitochondrial matrix. As a proof-of-principle study, thiol-cleavable biotin-assisted TurboID proteomics achieved remarkable intraorganelle spatial resolution with significantly enriched proteins localized in the mitochondrial inner membrane and mitochondrial matrix. In the experiment, the researchers used many compounds, for example, 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5COA of Formula: C10H16N2O3S)

5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.COA of Formula: C10H16N2O3S And it has been used as a vitamin supplement for the growth of Bacillus species.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

The author of 《Structure-based identification of novel CK2 inhibitors with a linear 2-propenone scaffold as anti-cancer agents》 were Qi, Xiaoqian; Zhang, Na; Zhao, Lijiao; Hu, Liming; Cortopassi, Wilian A.; Jacobson, Matthew P.; Li, Xitao; Zhong, Rugang. And the article was published in Biochemical and Biophysical Research Communications in 2019. HPLC of Formula: 7720-39-0 The author mentioned the following in the article:

Protein kinase CK2 has emerged as an attractive cancer therapeutic target. Previous studies have highlighted the challenge of optimizing CK2 ATP-competitive inhibitors that have low druggability due to their polycyclic ring scaffolds. Therefore the development of novel inhibitors with non-polycyclic scaffolds emerges as a promising strategy for drug discovery targeting CK2. In this current study, based on the similar predicted binding poses of the linear 2-propenone scaffold of isoliquiritigenin with that of the polycyclic inhibitor CX-4945, a series of 2-propenone derivatives containing an amine-substituted five-membered heterocycle and a benzoic acid were designed, synthesized and evaluated for their in vitro CK2 inhibition and anti-cancer activity. Compound 8b was found to be the most potent CK2 inhibitor (IC50 = 0.6 μM) with the anti-proliferative activity on HepG2 cancer cells (IC50 = 14 μM), compared to the activity of isoliquiritigenin (IC50 = 17 μM and 51 μM, resp.). Mol. docking was performed to understand the binding modes of the newly designed 2-propenone derivatives with CK2. Compound 8b formed the most favorable network of hydrogen bonds with both the hinge region and pos. area. Our results indicate that CK2 derivatives with a linear 2-propenone scaffold are promising candidates for anti-cancer drug discovery. After reading the article, we found that the author used 1H-Imidazol-2-amine(cas: 7720-39-0HPLC of Formula: 7720-39-0)

1H-Imidazol-2-amine(cas: 7720-39-0) belongs to anime. Reduction of nitro compounds, RNO2, by hydrogen or other reducing agents produces primary amines cleanly (i.e., without a mixture of products), but the method is mostly used for aromatic amines because of the limited availability of aliphatic nitro compounds. Reduction of nitriles and oximes (R2C=NOH) also yields primary amines.HPLC of Formula: 7720-39-0

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

The author of 《A biotin-guided fluorescent probe for dual-mode imaging of viscosity in cancerous cells and tumor tissues》 were Ren, Mingguang; Xu, Qingyu; Wang, Shoujuan; Liu, Lu; Kong, Fangong. And the article was published in Chemical Communications (Cambridge, United Kingdom) in 2020. Recommanded Product: 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid The author mentioned the following in the article:

A cancer cell targeted fluorescent viscosity probe has been designed and synthesized to specifically visualise viscosity changes in biotin receptor (BiR) pos. cells over biotin neg. cells via dual-mode fluorescence imaging: fluorescence intensity mode and fluorescence lifetime mode. In the experiment, the researchers used 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5Recommanded Product: 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid)

5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.Recommanded Product: 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid The biotin/avidin or biotin/streptavidin interaction is utilized in many labeling and purification schemes.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

《Ru(II)-Catalyzed and acidity-controlled tunable [5+1]/[5+2] annulation for building ring-fused quinazolines and 1,3-benzodiazepines》 was written by Yang, Yurong; Zhang, Kaixin; Yang, Jian; Zhu, Guoxun; Chen, Weijie; Zhang, Chao; Zhou, Zhi; Yi, Wei. Related Products of 4857-06-1 And the article was included in Chemical Communications (Cambridge, United Kingdom) in 2020. The article conveys some information:

The Ru(II)-catalyzed tunable [5+1]/[5+2] annulation of N-benzo[d]imidazole indolines with propargyl carbonates was realized for the divergent synthesis of ring-fused quinazolines and 1,3-benzodiazepines bearing various functional groups. These transformations represented an efficient and practical strategy in constructing complex heterocycles via diversified C-H functionalization. A distinctive acidity-controlled reaction manner was clarified to account for the chemoselectivity. In the experiment, the researchers used many compounds, for example, 2-Chloro-1H-benzo[d]imidazole(cas: 4857-06-1Related Products of 4857-06-1)

2-Chloro-1H-benzo[d]imidazole(cas: 4857-06-1) is an analog of benzimidazole that has been synthesized by Langmuir adsorption isotherm. It is a white crystalline solid that can be dissolved in water and hydrochloric acid. 2-Chloro-1H-benzo[d]imidazole inhibits the growth of herpes simplex virus by acting as a competitive inhibitor for the viral enzyme thymidine kinase, which catalyzes the conversion of thymine to thymidine.Related Products of 4857-06-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

V., Paleri Sandhya; V., Kakkotakath Muhammad Niyas; R., Karickal Haridas published their research in Research Journal of Chemistry and Environment in 2021. The article was titled 《Microwave assisted synthesis of benzimidazole derivatives using tetra butyl ammonium chloride as phase transfer catalyst, characterization, biological activities and molecular docking studies》.HPLC of Formula: 4857-06-1 The article contains the following contents:

Benzimidazole and its derivatives are of wide interest because of their diverse biol. activity and clin. applications. The heterocyclic imidazole core is a common moiety in a large number of natural products and pharmacol. active compounds and because of that they are possessing inhibitory activity as well as favorable selectivity ratio. Benzimidazoles exhibit significant activities like anti-microbial, anti-viral, anti-diabetic, anti-cancer activity, numerous antioxidants, anti-parasitic, anti-helmintics, antiproliferative, anti-HIV, anti-convulsant, anti-inflammatory, anti-hypertensive, anti-neoplastic, proton pump inhibitor and anti-trichinellosis. The synthesis of benzimidazole derivatives remains a focus of medicinal research. Here benzimidazole derivatives were synthesized using two phase system under microwave conditions using the phase transfer catalyst tetra Bu ammonium chloride (10% mol) in good yield. The mol. docking study against 4GQQ protein with synthesized benzimidazole derivatives was performed to see the necessary interactions responsible for anti-bacterial activity. The experimental process involved the reaction of 2-Chloro-1H-benzo[d]imidazole(cas: 4857-06-1HPLC of Formula: 4857-06-1)

2-Chloro-1H-benzo[d]imidazole(cas: 4857-06-1) binds to monoclonal antibodies, inhibiting their binding to their corresponding antigens. This activity may be due to its ability to bind covalently with amino groups on proteins and other molecules.HPLC of Formula: 4857-06-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Recommanded Product: 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acidIn 2019 ,《Making routine native SAD a reality: lessons from beamline X06DA at the Swiss Light Source》 was published in Acta Crystallographica, Section D: Structural Biology. The article was written by Basu, Shibom; Finke, Aaron; Vera, Laura; Wang, Meitian; Olieric, Vincent. The article contains the following contents:

Native single-wavelength anomalous dispersion (SAD) is the most attractive de novo phasing method in macromol. crystallog., as it directly utilizes intrinsic anomalous scattering from native crystals. However, the success of such an experiment depends on accurate measurements of the reflection intensities and therefore on careful data-collection protocols. Here, the low-dose, multiple-orientation data-collection protocol for native SAD phasing developed at beamline X06DA (PXIII) at the Swiss Light Source is reviewed, and its usage over the last four years on conventional crystals (>50μm) is reported. Being exptl. very simple and fast, this method has gained popularity and has delivered 45 de novo structures to date (13 of which have been published). Native SAD is currently the primary choice for exptl. phasing among X06DA users. The method can address challenging cases: here, native SAD phasing performed on a streptavidin-biotin crystal with P21 symmetry and a low Bijvoet ratio of 0.6% is highlighted. The use of intrinsic anomalous signals as sequence markers for model building and the assignment of ions is also briefly described. The experimental part of the paper was very detailed, including the reaction process of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5Recommanded Product: 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid)

5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.Recommanded Product: 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid And it has been used as a vitamin supplement for the growth of Bacillus species.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

《Microscopic structural and dynamic features in triphilic room temperature ionic liquids》 was written by Celso, Fabrizio Lo; Appetecchi, Giovanni B.; Simonetti, Elisabetta; Zhao, Man; Castner, Edward W. Jr.; Keiderling, Uwe; Gontrani, Lorenzo; Triolo, Alessandro; Russina, Olga. Safety of 1-Methyl-1H-imidazoleThis research focused onX ray neutron scattering viscosity diffusion relaxation fragility; fluorinated methylimidazolium ionic liquid microstructure; fluorous tail; ionic liquid; molecular dynamics (MD); neutron scattering; triphilic. The article conveys some information:

Here we report a thorough investigation of the microscopic and mesoscopic structural organization in a series of triphilic fluorinated room temperature ionic liquids, namely [1-alkyl,3-methylimidazolium][(trifluoromethanesulfonyl)(nonafluorobutylsulfonyl)imide], with alkyl = Et, Bu, octyl ([Cnmim][IM14], n = 2, 4, 8), based on the synergic exploitation of X-ray and Neutron Scattering and Mol. Dynamics simulations. This study reveals the strong complementarity between X-ray/neutron scattering in detecting the complex segregated morphol. in these systems at mesoscopic spatial scales. The use of MD simulations delivering a very good agreement with exptl. data allows us to gain a robust understanding of the segregated morphol. The structural scenario is completed with determination of dynamic properties accessing the diffusive behavior and a relaxation map is provided for [C2mim][IM14] and [C8mim][IM14], highlighting their natures as fragile glass formers. The experimental process involved the reaction of 1-Methyl-1H-imidazole(cas: 616-47-7Safety of 1-Methyl-1H-imidazole)

1-Methyl-1H-imidazole(cas: 616-47-7) is used as a precursor for the synthesis of pyrrole-imidazole polyamides, ionic liquids such as 1-butyl-3-methylimidazolium hexafluorophosphate.Safety of 1-Methyl-1H-imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

《Selective C-H trifluoromethylation of benzimidazoles through photoredox catalysis》 was written by Gao, Guo-Lin; Yang, Chao; Xia, Wujiong. Category: imidazoles-derivativesThis research focused ontrifluoromethyl benzoimidazole preparation; benzimidazole trifluoromethylation photoredox catalyst togni reagent. The article conveys some information:

A new strategy for the preparation of trifluoromethyl benzimidazoles I (R = H, 5,6-di-Me, 5-Cl, etc.; R1 = H, CH3, C6H5CH2, etc.; X = H, CH2OCH3, pyridin-2-yl, etc.) via visible light induced C-H trifluoromethylation at C4 of benzimidazoles II using Togni’s reagent in the presence of fac-Ir(ppy)3 has been presented. Its advantages are operational simplicity, mild reaction conditions, low catalyst loading and wide substrate scope, in which electron-withdrawing, electron-donating groups and different protecting groups are tolerated. After reading the article, we found that the author used 2-Chloro-1H-benzo[d]imidazole(cas: 4857-06-1Category: imidazoles-derivatives)

2-Chloro-1H-benzo[d]imidazole(cas: 4857-06-1) is an analog of benzimidazole that has been synthesized by Langmuir adsorption isotherm. It is a white crystalline solid that can be dissolved in water and hydrochloric acid. 2-Chloro-1H-benzo[d]imidazole inhibits the growth of herpes simplex virus by acting as a competitive inhibitor for the viral enzyme thymidine kinase, which catalyzes the conversion of thymine to thymidine.Category: imidazoles-derivatives

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

《UV photostability of three 2-aminoazoles with key roles in prebiotic chemistry on the early earth》 was written by Todd, Zoe R.; Szabla, Rafal; Szostak, Jack W.; Sasselov, Dimitar D.. HPLC of Formula: 7720-39-0This research focused onUV photostability aminoazole prebiotic chem early earth. The article conveys some information:

Three related mols. in the 2-aminoazole family are potentially important for prebiotic chem.: 2-aminooxazole, 2-aminoimidazole, and 2-aminothiazole, which can provide critical functions as an intermediate in nucleotide synthesis, a nucleotide activating agent, and a selective agent, resp. Here, we examine the wavelength-dependent photodegradation of these three mols. under mid-range UV light (210-290 nm). We then assess the implications of the observed degradation rates for the proposed prebiotic roles of these compounds We find that all three 2-aminoazoles degrade under UV light, with half lives ranging from ≈7-100 h under a solar-like spectrum. 2-Aminooxazole is the least photostable, while 2-aminoimidazole is the most photostable. The relative photostabilities are consistent with the order in which these mols. would be used prebiotically: AO is used first to build nucleotides and AI is used last to activate them. In the experimental materials used by the author, we found 1H-Imidazol-2-amine(cas: 7720-39-0HPLC of Formula: 7720-39-0)

1H-Imidazol-2-amine(cas: 7720-39-0) belongs to anime. Milder oxidation, using reagents such as NaOCl, can remove four hydrogen atoms from primary amines of the type RCH2NH2 to form nitriles (R―C≡N), and oxidation with reagents such as MnO2 can remove two hydrogen atoms from secondary amines (R2CH―NHR′) to form imines (R2C=NR′). Tertiary amines can be oxidized to enamines (R2C=CHNR2) by a variety of reagents.HPLC of Formula: 7720-39-0

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Elmali, A.; Elerman, Y.; Eren, G.; Guemues, F.; Svoboda, I. published an article on February 28 ,2005. The article was titled 《The crystal structures of 2-(3′-hydroxypropyl)benzimidazolium hexa- and tetrachloroplatinate》, and you may find the article in Zeitschrift fuer Naturforschung, B: Chemical Sciences.Quality Control of 3-(1H-Benzo[d]imidazol-2-yl)propan-1-ol The information in the text is summarized as follows:

2-(3′-Hydroxypropyl)benzimidazolium (Hhpb) hexa- and tetrachloroplatinate (Co10H13N2O)2·[PtCl6] (I) and (C10H13N2O)2·[PtCl4] (II) were synthesized and their crystal structures determined I is monoclinic, space group P21/n, a 8.800(1), b 14.389(2), c 10.264(2) Å, β 98.540(10)°, and Z = 2. II is triclinic, space group P1̅, a 7.8480(10), b 9.0460(10), c 9.6980(10) Å, α 65.420(10), β 68.810(10), γ 76.770(1)°, and Z = 1. In both compounds, the Pt atoms reside at a center of inversion. I and II are comprised of 2-(3′-hydroxypropyl)benzimidazolium (Hhpb)+:(C10H12N2O)+ and [PtCl6]2- and [PtCl4]2- ions, resp., linked by intermol. H bonds N···Cl [range from 3.428(3) to 3.584(4) Å], N···O [2.769(5) Å] and O···Cl [3.338(4) and 3.321(3) Å] for I, and N···Cl [3.162(7) Å], N···O [2.749(8) Å] and O···Cl [3.289(6) Å] for II. The experimental part of the paper was very detailed, including the reaction process of 3-(1H-Benzo[d]imidazol-2-yl)propan-1-ol(cas: 2403-66-9Quality Control of 3-(1H-Benzo[d]imidazol-2-yl)propan-1-ol)

3-(1H-Benzo[d]imidazol-2-yl)propan-1-ol(cas: 2403-66-9) belongs to imidazoles.Imidazole rings are part of unnatural cyclic peptides and are used as ester isosteres in peptidomimetic studies.
However, the application of imidazoles is not limited to the field of peptides and peptidomimetics. Quality Control of 3-(1H-Benzo[d]imidazol-2-yl)propan-1-ol

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem