Month: October 2022

Rehman, Mobeen Ur; Chong, Kil To published an article in 2020, the title of the article was DNA6mA-MINT: DNA-6mA modification identification neural tool.Recommanded Product: N-Methyl-7H-purin-6-amine And the article contains the following content:

DNA N6-methyladenine (6mA) is part of numerous biol. processes including DNA repair, DNA replication, and DNA transcription. The 6mA modification sites hold a great impact when their biol. function is under consideration. Research in biochem. experiments for this purpose is carried out and they have demonstrated good results. However, they proved not to be a practical solution when accessed under cost and time parameters. This led researchers to develop computational models to fulfill the requirement of modification identification. In consensus, we have developed a computational model recommended by Chou’s 5-steps rule. The Neural Network (NN) model uses convolution layers to extract the high-level features from the encoded binary sequence. These extracted features were given an optimal interpretation by using a Long Short-Term Memory (LSTM) layer. The proposed architecture showed higher performance compared to state-of-the-art techniques. The proposed model is evaluated on Mus musculus, Rice, and “Combined-species” genomes with 5- and 10-fold cross-validation. Further, with access to a user-friendly web server, publicly available can be accessed freely. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: N-Methyl-7H-purin-6-amine

The Article related to n6 methyladenine genome dna replication convolutional neural network, chou’s 5-steps rule, convolution neural network (cnn), dna n6-methyladenine, long short-term memory (lstm), computational biology and other aspects.Recommanded Product: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2021, Geng, Chengbao; Fan, Lu-an; Niu, Hongyan; Liu, Lijia; Zhao, Fangbo; Zhang, Jiaming; Dong, Hongxing; Yu, Shuili published an article.Category: imidazoles-derivatives The title of the article was Improved anti-organic fouling and antibacterial properties of PVDF ultrafiltration membrane by one-step grafting imidazole-functionalized graphene oxide. And the article contained the following:

At present, membrane fouling is a thorny issue that limits the development of polyvinylidene fluoride (PVDF) composite membrane, which seriously affects its separation performance and service lifespan. Herein, an imidazole-functionalized graphene oxide (Im-GO) with hydrophilicity and antibacterial performance was synthesized, and it was used as a modifier to improve the anti-organic fouling and antibacterial properties of PVDF membrane. The anti-organic fouling test showed that the maximum flux recovery ratios against bovine serum albumin and humic acid were 88.9% and 94.5%, resp. Conspicuously, the grafted imidazole groups could effectively prevent the bacteria from growing on the membrane surface. It was gratifying that the antibacterial modifier Im-GO was almost not lost from the hybrid membranes even by the ultrasonic treatment, which was different from the conventional release-killing antibacterial agents. Owing to the long-term anti-organic fouling and antibacterial properties, Im-GO/PVDF hybrid membranes exhibit a great application potential in the fields of rough separation and concentration of biomedical products. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Category: imidazoles-derivatives

The Article related to escherichia pvdf imidazole graphene oxide humic acid hydrophilicity antibacterial, anti-organic fouling, antibacterial, hydrophilicity, imidazole-functionalized graphene oxide, polyvinylidene fluoride and other aspects.Category: imidazoles-derivatives

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Takahashi, Yusuke; Shoura, Massa; Fire, Andrew; Morishita, Shinichi published an article.SDS of cas: 443-72-1 The title of the article was Context-dependent DNA polymerization effects can masquerade as DNA modification signals. And the article contained the following:

Single mol. measurements of DNA polymerization kinetics provide a sensitive means to detect both secondary structures in DNA and deviations from primary chem. structure as a result of modified bases. In one approach to such anal., deviations can be inferred by monitoring the behavior of DNA polymerase using single-mol., real-time sequencing with zero-mode waveguide. This approach uses a Single Mol. Real Time (SMRT)-sequencing measurement of time between fluorescence pulse signals from consecutive nucleosides incorporated during DNA replication, called the interpulse duration (IPD). In this paper we present an anal. of loci with high IPDs in two genomes, a bacterial genome (E. coli) and a eukaryotic genome (C. elegans). To distinguish the potential effects of DNA modification on DNA polymerization speed, we paired an anal. of native genomic DNA with whole-genome amplified (WGA) material in which DNA modifications were effectively removed. Adenine modification sites for E. coli are known and we observed the expected IPD shifts at these sites in the native but not WGA samples. For C. elegans, such differences were not observed Instead, we found a number of novel sequence contexts where IPDs were raised relative to the average IPDs for each of the four nucleotides, but for which the raised IPD was present in both native and WGA samples. The latter results argue strongly against DNA modification as the underlying driver for high IPD segments for C. elegans, and provide a framework for separating effects of DNA modification from context-dependent DNA polymerase kinetic patterns inherent in underlying DNA sequence for a complex eukaryotic genome. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to dna polymerization effect masquerade modification signal, dna n6-methyladenine, dna modification, dna polymerization, non-b dna, single-molecule real-time (smrt) sequencing, whole genome amplification and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On September 30, 2021, Xue, Tian; Zhang, Shengli; Qiao, Huijuan published an article.Recommanded Product: 443-72-1 The title of the article was i6mA-VC: A Multi-Classifier Voting Method for the Computational Identification of DNA N6-methyladenine Sites. And the article contained the following:

Abstract: DNA N6-methyladenine (6 mA), as an essential component of epigenetic modification, cannot be neglected in genetic regulation mechanism. Most of the established machine learning methods have a single dataset. Although some of them have achieved cross-species prediction, their results are not satisfactory. Therefore, we designed a novel statistical model called i6mA-VC to improve the accuracy for 6 mA sites. On the one hand, kmer and binary encoding are applied to extract features, and then gradient boosting decision tree (GBDT) embedded method is applied as the feature selection strategy. After fusing the two optimal features, a voting classifier based on gradient boosting decision tree (GBDT), light gradient boosting machine (LightGBM) and multilayer perceptron classifier (MLPC) is constructed for final classification and prediction. The accuracy of Rice dataset and M.musculus dataset with five-fold cross-validation are 0.888 and 0.967, resp. The cross-species dataset is selected as independent testing dataset, and the accuracy reaches 0.848. Through rigorous experiments, it is demonstrated that the proposed predictor is convincing and applicable. The development of i6mA-VC predictor will become an effective way for the recognition of N6-methyladenine sites, and it will also be beneficial for biol. geneticists to further study gene expression and DNA modification. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: 443-72-1

The Article related to n6methyladenine machine learning dna sequence genetics, dna n6-methyladenine sites, light gradient boosting machine, multilayer perceptron classifier, ring-function-hydrogen-chemical properties, voting and other aspects.Recommanded Product: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Ding, Fangwei; Jiang, Yanqiu; Gan, Shaoyan; Bao, Robert Li-Yuan; Lin, Kaifeng; Shi, Lei published an article in 2017, the title of the article was B(C6F5)3-Catalyzed Deoxygenation of Sulfoxides and Amine N-Oxides with Hydrosilanes.Synthetic Route of 73590-85-9 And the article contains the following content:

An efficient strategy for the deoxygenation of sulfoxides and amine N-oxides by using B(C6F5)3 and hydrosilanes was developed. This method provided the corresponding aromatic and aliphatic sulfides/amines in good to high yields and showed good functional-group tolerance under mild conditions. This protocol should be very useful in the future because of its ease of operation, the environmentally friendly reaction conditions and wide scope. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Synthetic Route of 73590-85-9

The Article related to sulfide green preparation, sulfoxide deoxygenation phenylsilane tris pentafluorophenyl borane catalyst, amine green preparation, deoxygenation amine oxide phenylsilane tris pentafluorophenyl borane catalyst and other aspects.Synthetic Route of 73590-85-9

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On February 28, 2022, Wang, Dong-En; You, Shangqi; Huo, Wenjing; Han, Xiang; Xu, Huiyun published an article.Safety of N-(3-Aminopropyl)-imidazole The title of the article was Colorimetric detection of alkaline phosphatase activity based on pyridoxal phosphate-induced chromatic switch of polydiacetylene nanoliposomes. And the article contained the following:

A colorimetric assay based on polydiacetylenes (PDA) nano-liposomes is reported for facile and sensitive detection of alk. phosphatase (ALP) activity. The critical basis of this method is that the interaction of pyridoxal phosphate (PLP) with nitrogenous group functionalized PDA nano-liposomes induces distinct blue-to-red color changes of PDA nano-liposomes. In the presence of ALP, as a nature substrate, PLP is enzymically hydrolyzed to form pyridoxal, which cannot interact with PDA nano-liposomes. As a result, the concentration of PLP is reduced and the color change of PDA nano-liposomes is retarded, which is associated with ALP level. Under optimal conditions, the proposed method showed good linear relationship with ALP activity in the range 10-200 U/L with a limit of detection of 2.8 U/L. The detection process could be vividly observed with the naked eye. Addnl. attempts by using the method for the evaluation of inhibitor efficiency were also achieved with satisfying results. The method was further challenged with real human serum samples, showing consistent results when compared with a com. standard assay kit. Such simple and easy-to-use approach may provide a new alternative for clin. and biol. detection of ALP. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Safety of N-(3-Aminopropyl)-imidazole

The Article related to polydiacetylene nanoliposome chromatic switch pyridoxal phosphate alk phosphatase colorimetry, alkaline phosphatase, colorimetric assay, conjugated polymer, nano-liposomes, polydiacetylene, pyridoxal phosphate and other aspects.Safety of N-(3-Aminopropyl)-imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Zhang, Zhongwang; Wang, Lidong published an article in 2022, the title of the article was Using Chou’ s 5-steps rule to identify N6-methyladenine sites by ensemble learning combined with multiple feature extraction methods.Formula: C6H7N5 And the article contains the following content:

N6-methyladenine (m6A), a type of modification mostly affecting the downstream biol. functions and determining the levels of gene expression, is mediated by the methylation of adenine in nucleic acids. It is also a key factor for influencing biol. processes and has attracted attention as a target for treating diseases. Here, an ensemble predictor named as TL-Methy, was developed to identify m6A sites across the genome. TL-Methy is a 2-level machine learning method developed by combining the support vector machine model and multiple features extraction methods, including nucleic acid composition, di-nucleotide composition, tri-nucleotide composition, position-specific trinucleotide propensity, Bi-profile Bayes, binary encoding, and accumulated nucleotide frequency. For Homo sapiens, TL-Methy method reached the accuracy of 91.68% on jackknife test and of 92.23% on 10-fold cross validation test; For Mus musculus, TL-Methy method achieved the accuracy of 93.66% on jackknife test and of 97.07% on 10-fold cross validation test; For Saccharomyces cerevisiae, TL-Methy method obtained the accuracy of 81.57% on jackknife test and of 82.54% on 10-fold cross validation test; For rice genome, TL-Methy method achieved the accuracy of 91.87% on jackknife test and of 93.04% on 10-fold cross validation test. The results via these two test approaches demonstrated the robustness and practicality of our TL-Methy model. The TL-Methy model may be as a potential method for m6A site identification.Communicated by Ramaswamy H. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to saccharomyces cerevisiae mus musculus methyladenine jackknife test feature extraction, 10-fold cross validation test, 2-level method, n6-methyladenine, feature extraction, jackknife test, support vector machine and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2017, Jang, Hyun-Hee; Ryu, Sang-Hoon; Le, Thien-Kim; Doan, Tiep Thi My; Nguyen, Thi Huong Ha; Park, Ki Deok; Yim, Da-Eun; Kim, Dong-Hyun; Kang, Choong-Kyung; Ahn, Taeho; Kang, Hyung-Sik; Yun, Chul-Ho published an article.Synthetic Route of 73590-85-9 The title of the article was Regioselective C-H hydroxylation of omeprazole sulfide by Bacillus megaterium CYP102A1 to produce a human metabolite. And the article contained the following:

Objectives: To find a simple enzymic strategy for the efficient synthesis of the expensive 5′-hydroxyomeprazole sulfide, a recently identified minor human metabolite, from omeprazole sulfide, which is an inexpensive substrate. Results: The practical synthetic strategy for the 5′-OH omeprazole sulfide was accomplished with a set of highly active CYP102A1 mutants, which were obtained by blue colony screening from CYP102A1 libraries with a high conversion yield. The mutant and even the wild-type enzyme of CYP102A1 catalyzed the high regioselective (98 %) C-H hydroxylation of omeprazole sulfide to 5′-OH omeprazole sulfide with a high conversion yield (85-90 %). Conclusions: A highly efficient synthesis of 5′-OH omeprazole sulfide was developed using CYP102A1 from Bacillus megaterium as a biocatalyst. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Synthetic Route of 73590-85-9

The Article related to regioselective carbon hydrogen hydroxylation omeprazole sulfide bacillus megaterium cyp102a1, 5-hydroxyomeprazole sulphide, cyp102a1 mutant, human metabolite, hydroxylation, omeprazole sulfide, regioselectivity and other aspects.Synthetic Route of 73590-85-9

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem