Month: October 2022

Camel, Benjamin R.; Jose, Davis; Meze, Katarina; Dang, Anson; von Hippel, Peter H. published the artcile< Mapping DNA conformations and interactions within the binding cleft of bacteriophage T4 single-stranded DNA binding protein (gp32) at single nucleotide resolution>, Electric Literature of 452-06-2, the main research area is DNA binding domain bacteriophage T4 gp32 single nucleotide resolution.

In this study, we use single-stranded DNA (oligo-dT) lattices that have been position-specifically labeled with monomer or dimer 2-aminopurine (2-AP) probes to map the local interactions of the DNA bases with the nucleic acid binding cleft of gp32, the single-stranded binding (ssb) protein of bacteriophage T4. Three complementary spectroscopic approaches are used to characterize these local interactions of the probes with nearby nucleotide bases and amino acid residues at varying levels of effective protein binding cooperativity, as manipulated by changing lattice length. These include: (i) examining local quenching and enhancing effects on the fluorescence spectra of monomer 2-AP probes at each position within the cleft; (ii) using acrylamide as a dynamic-quenching additive to measure solvent access to monomer 2-AP probes at each ssDNA position; and (iii) employing CD spectra to characterize changes in exciton coupling within 2-AP dimer probes at specific ssDNA positions within the protein cleft. The results are interpreted in part by what we know about the topol. of the binding cleft from crystallog. studies of the DNA binding domain of gp32 and provide addnl. insights into how gp32 can manipulate the ssDNA chain at various steps of DNA replication and other processes of genome expression.

Nucleic Acids Research published new progress about Bacteriophage T4 protein Gp32 Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Electric Literature of 452-06-2.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Gao, Lingling; Nie, Xin; Gou, Rui; Hu, Yuexin; Dong, Hui; Li, Xiao; Lin, Bei published the artcile< Exosomal ANXA2 derived from ovarian cancer cells regulates epithelial-mesenchymal plasticity of human peritoneal mesothelial cells>, Quality Control of 6823-69-4, the main research area is ANXA ovarian cancer epithelial mesenchymal plasticity human peritoneal mesothelium; ANXA2; Implantation and metastasis; epithelial-mesenchymal plasticity; exosome; ovarian cancer.

Ovarian cancer, one of the malignant gynaecol. tumors with the highest mortality rate among female reproductive system, is prone to metastasis, recurrence and chemotherapy resistance, causing a poor prognosis. Exosomes can regulate the epithelial-mesenchymal plasticity of tumor cells, remodel surrounding tumor microenvironment, and affect tumor cell proliferation, invasion and metastasis. However, the function and mechanism of exosomes in the i.p. implantation of ovarian cancer remain unclear. In this study, exosomal annexin A2 (ANXA2) derived from ovarian cancer cells was co-cultured with human peritoneal mesothelial (HMrSV5) cells; functional experiments were conducted to explore the effects of exosomal ANXA2 on the biol. behavior of HMrSV5 and the related mechanisms. This study showed that ANXA2 in ovarian cancer cells can be transferred to HMrSV5 cells through exosomes, exosomal ANXA2 can not only promote the migration, invasion and apoptosis of HMrSV5 cells, but also regulates morphol. changes and fibrosis of HMrSV5 cells. Furthermore, ANXA2 promotes the mesothelial-mesenchymal transition (MMT) and degradation of the extracellular matrix of HMrSV5 cells through PI3K/AKT/mTOR pathway, finally affects pre-metastasis microenvironment of ovarian cancer, which provides a new theor. basis for the mechanism of i.p. implantation and metastasis of ovarian cancer.

Journal of Cellular and Molecular Medicine published new progress about Annexin A2 Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Quality Control of 6823-69-4.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Ambati, Srinivasa Rao; Patel, Jeevan Lal; Gudala, Satish; Chandrakar, Komal; Penta, Santhosh; Mahapatra, S. P.; Banerjee, Subhash published the artcile< Synthesis of novel coumarinyl-pyrido[2,3-d]pyrimidine-2,4-diones using task-specific magnetic ionic liquid, [AcMIm]FeCl4 as catalyst>, Application In Synthesis of 700370-07-6, the main research area is chloroacrolein coumarinyl heterocyclization uracil reusable ionic liquid catalyst; coumarinyl pyridopyrimidinedione preparation green chem.

An acid-functionalized magnetic ionic liquid, 1-carboxymethyl-3-methylimidazolium tetrachloroferrate, was utilized for the synthesis of a series of novel highly functionalized (coumarinyl)pyrido[2,3-d]pyrimidine-2,4-diones I (R1 = H,Br, Cl, MeO; R2 = H, Cl, Br; R3 = Me, cyclopropyl) by the reactions of various 3-chloro-3-(2-oxo-2H-chromen-3-yl)acrylaldehydes II with functionalized 6-aminouracils III. The central point of the present procedure was the use of task-specific acidic ionic liquid which acts as catalyst as well as reaction medium thus avoiding the use of organic solvent and/or protic acid catalyst. The other major advantages of the protocol were (i) shorter reaction time (1 h), (ii) easy work up procedure, (iii) excellent yields of products (91-94%) and (iv) recyclability of the catalyst. The compounds I were identified using FT-IR, 1H-NMR and 13C-NMR and mass spectroscopic studies.

Synthetic Communications published new progress about Coumarins Role: RCT (Reactant), SPN (Synthetic Preparation), RACT (Reactant or Reagent), PREP (Preparation). 700370-07-6 belongs to class imidazoles-derivatives, and the molecular formula is C6H9ClN2O2, Application In Synthesis of 700370-07-6.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Okuro, Renata Tiemi; Machado, Mariana Nascimento; Casquilho, Natalia Vasconcelos; Jardim-Neto, Alcendino; Roncally-Carvalho, Alysson; Atella, Georgia Correa; Zin, Walter Araujo published the artcile< The role of sphingolipid metabolism disruption on lipopolysaccharide-induced lung injury in mice>, Category: imidazoles-derivatives, the main research area is lung injury sphingolipid metabolism disruption; Ceramide; Enzyme inhibitors; Inflammation; Lipopolysaccharide; Lung injury; Sphingolipid.

This study assessed pulmonary outcomes generated by inhibiting key enzymes of sphingolipid metabolism pathways related to ceramide synthesis in a murine model of lung injury induced by lipopolysaccharide (LPS). C57BL/6 male adult mice received LPS intratracheally and the expressions of acid sphingomyelinase (ASM), neutral sphingomyelinase (NSM), serine palmitoyl transferase (SPT) and dihydroceramide synthase (DS) were assessed at 2, 4, 6, 12 and 24 h after LPS instillation in lung homogenate (n = 30). The pharmacol. inhibition of ASM, NSM, SPT and DS were assayed in other mice groups by three different doses of desipramine, GW4869, myriocin and fumonisin, resp. (n = 90). Their most EDs were administered i.p. 1 or 2 h before LPS to different animal groups (n = 120). Mice underwent determination of pulmonary mechanics, lung histopathol. aspects and apoptosis. The expression levels of the enzymes reached their peak at 2-4 h after LPS administration. ASM inhibition attenuated alveolar collapse and GW4869 decreased lung elastance, proinflammatory cytokines’ levels and was more effective to improve alveolar collapse than desipramine. On the other hand, SPT blockage aggravated lung lesion and no effects it was observed with fumonisin. Moreover, simultaneous administration of inhibitors (desipramine + GW4869, myriocin + fumonisin and all inhibitors together) resulted in no changes. Blockage of sphingomyelinases and the de novo pathways improved and aggravated lung injury, resp., putatively suggesting specific targets to therapeutic strategies in LPS-induced lung injury.

Pulmonary Pharmacology & Therapeutics published new progress about Alveolus. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Category: imidazoles-derivatives.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Asoh, Kohsuke; Kohchi, Masami; Hyoudoh, Ikumi; Ohtsuka, Tatsuo; Masubuchi, Miyako; Kawasaki, Kenichi; Ebiike, Hirosato; Shiratori, Yasuhiko; Fukami, Takaaki A.; Kondoh, Osamu; Tsukaguchi, Toshiyuki; Ishii, Nobuya; Aoki, Yuko; Shimma, Nobuo; Sakaitani, Masahiro published the artcile< Synthesis and structure-activity relationships of novel benzofuran farnesyltransferase inhibitors>, Name: 5-Bromo-1-methyl-1H-imidazole, the main research area is benzofuran imidazolyl preparation farnesyltransferase inhibitor.

A series of imidazolylbenzofuran-based farnesyltransferase inhibitors have been designed and synthesized as antitumor agents. Among them, I showed the most potent enzyme inhibitory activity (IC50 = 1.1 nM) and antitumor activity in human cancer xenografts in mice.

Bioorganic & Medicinal Chemistry Letters published new progress about Crystal structure. 1003-21-0 belongs to class imidazoles-derivatives, and the molecular formula is C4H5BrN2, Name: 5-Bromo-1-methyl-1H-imidazole.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Pohida, Katherine; Maloney, David J.; Mott, Bryan T.; Rai, Ganesha published the artcile< Room-Temperature, Copper-Free Sonogashira Reactions Facilitated by Air-Stable, Monoligated Precatalyst [DTBNpP] Pd(crotyl)Cl>, Related Products of 1003-21-0, the main research area is Sonogashira coupling palladium DTBNpP crotyl catalyzed.

A novel application of [DTBNpP] Pd(crotyl)Cl (DTBNpP = di-tert-butylneopentylphosphine) (P2), an air-stable, com.-available palladium precatalyst that allows rapid access to a mono-ligated state, has been identified for room temperature, copper-free Sonogashira couplings of challenging aryl bromides and alkynes. The mild reaction conditions with TMP in DMSO afford up to 97% yields, excellent functional group tolerability, and broad reaction compatibility with access to one-pot indole formation.

ACS Omega published new progress about Alkynes Role: RCT (Reactant), RACT (Reactant or Reagent). 1003-21-0 belongs to class imidazoles-derivatives, and the molecular formula is C4H5BrN2, Related Products of 1003-21-0.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Matsuki-Fukushima, Miwako; Fujikawa, Kaoru; Inoue, Satoshi; Nakamura, Masanori published the artcile< Expression and localization of CD63 in the intracellular vesicles of odontoblasts>, SDS of cas: 6823-69-4, the main research area is expression intracellular vesicle odontoblast; CD63; Dentinogenesis; Odontoblast; Tooth development; Vesicle transport.

We hypothesized that odontoblasts release exosomes as well as dental pulp cells and focused on the exosome membrane marker CD63. Odontoblasts are well-differentiated mesenchymal cells that produce dentin. Dental pulp, a tissue complex formed with odontoblasts, releases exosomes to epithelial cells and stimulates their differentiation to ameloblasts. However, the localization of CD63 in differentiated odontoblasts is poorly understood. Therefore, herein, we aimed to reveal the expression of CD63 in odontoblasts during tooth development. We first investigated the localization of CD63 in mouse incisors and molars using immunofluorescence. In adult mouse incisors, the anti-CD63 antibody was pos. in mature odontoblasts and dental pulp cells but not in pre-odontoblasts along the ameloblasts in the apical bud. Addnl., the anti-CD63 antibody was observed as a vesicular shape in the apical area of odontoblast cytosol and inside Tomes’ fibers. The anti-CD63 antibody-pos. vesicles were also observed using immunoelectron microscopy. Moreover, during mouse mandibular molar tooth morphogenesis (E16 to postnatal 6 wk), labeling of anti-CD63 antibody was pos. in the odontoblasts at E18. In contrast, the anti-CD63 antibody was pos. in the dental pulp after postnatal day 10. Furthermore, anti-CD63 antibody was merged with the multivesicular body marker Rab7 in dental pulp tissues but not with the lysosome marker Lamp1. Finally, we determined the effect of a ceramide-generation inhibitor GW4869 on the mouse organ culture of tooth germ in vitro. After 28 days of GW4869 treatment, both CD63 and Rab7 were neg. in Tomes’ fibers, but were pos. in control odontoblasts. These results suggest that CD63-pos. vesicular organelles are important for mouse tooth morphogenesis.

Histochemistry and Cell Biology published new progress about Adult, mammalian. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, SDS of cas: 6823-69-4.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Gong, Liao-Kuo; Du, Ke-Zhao; Huang, Xiao-Ying published the artcile< PbX2(OOCMMIm) (X = Cl, Br): photoluminescent organic-inorganic hybrid lead halide compounds with high proton conductivity>, Name: 1-carboxymethyl-3-methylimidazolium chloride, the main research area is lead carboxymethylmethylimidazolium halide preparation photoluminescence proton conductivity; crystal structure lead carboxymethylmethylimidazolium chloride bromide inorganic organic hybrid.

Differences in the electronegativity and hydrophilicity of halogens lead to differences in proton-conducting and photoluminescence properties in hybrid organic-inorganic lead halide compounds of [PbX2(OOCMMIm)]n (X = Cl 1, Br 2, HOOCMMIm = 1-carboxymethyl-3-methylimidazolium).

Dalton Transactions published new progress about Activation energy (proton-transport). 700370-07-6 belongs to class imidazoles-derivatives, and the molecular formula is C6H9ClN2O2, Name: 1-carboxymethyl-3-methylimidazolium chloride.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Lu, Yixia; Hu, Jian; Zeng, Yanbo; Zhu, Ying; Wang, Hailong; Lei, Xiaoling; Huang, Shisi; Guo, Longhua; Li, Lei published the artcile< Electrochemical determination of rutin based on molecularly imprinted poly (ionic liquid) with ionic liquid-graphene as a sensitive element>, Category: imidazoles-derivatives, the main research area is rutin graphene molecularly imprinted polyionic liquid electrochem sensor.

A novel electrochem. sensor for rutin determination based on molecularly imprinted poly (ionic liquid) (MIPIL)/ionic liquid-graphene (IL-GR) modified electrode was developed. MIPIL was synthesized via free radical polymerization using rutin as the template, 1-allyl-3-Et imidazolium bromide ([AEIm]Br) as the functional monomer and 1,4-butanediyl-3,3′-bis-L-vinylimidazolium dibromide ([V2C4(mim)2]Br2) as the crosslinker. The composite of IL-GR as an electrode sensitive element was prepared using carboxymethyl-3-methylimidazolium chloride ([HO2CMMIm]Cl) by one-step ultrasound method. Fourier transform IR spectroscopy and scanning electron microscope were used to characterize IL-GR and MIPIL. The increased surface area and conductivity of IL-GR/GCE improved the sensitivity of rutin sensor. Under the optimum condition, good linearity for rutin anal. by the MIPIL-based sensor was obtained from 0.03 to 1μM. A low limit of detection for rutin was 0.01μM (S/N = 3). The MIPIL-based sensor demonstrated superiority on DPV response compared to the imprinted sensors based on traditional crosslinkers. The proposed sensor presented good selectivity for rutin and successfully applied for rutin determination in tablets with RSD of 3.05% and the average recovery range was 98.3%.

Sensors and Actuators, B: Chemical published new progress about Atom transfer radical polymerization. 700370-07-6 belongs to class imidazoles-derivatives, and the molecular formula is C6H9ClN2O2, Category: imidazoles-derivatives.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Peng, Lu; Wang, Yawen; Yang, Bingwei; Qin, Qi; Song, Erqun; Song, Yang published the artcile< Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop>, Recommanded Product: p-Benzenediacrylanilide, 4′,4′′-di-2-imidazolin-2-yl-, dihydrochloride, the main research area is polychlorinated biphenyl quinone MLKL protein phosphorylation exosome; Environmental pollution; Exosome; MLKL; Necroptosis; PCBs.

Polychlorinated biphenyls (PCBs) are one of the most refractory organic environmental pollutants that ubiquitous existence in nature. Due to the polymorphism of their metabolic pathway and corresponding downstream metabolites, PCBs’ toxicities are complicated and need extended investigation. In the present study, we discovered a novel regulatory mechanism of PCB quinone metabolite-driven programmed cell death (PCD), namely, necroptosis. We first confirmed that PCB quinone induces cancerous HeLa and MDA-MB-231 cells necroptosis via the phosphorylation of mixed lineage kinase domain-like MLKL (p-MLKL). Then, we found that PCB quinone-stimulated p-MLKL enhances exosome biogenesis and secretion. Exosome interacts with p-MLKL and releases p-MLKL to the outside of the cell, and ultimately alleviating PCB quinone-induced necroptosis. The inhibition of exosome secretion by GW4869 significantly elevated necroptotic level, indicating the establishment of a short neg. feedback loop of MLKL-exosome secretion upon PCB quinone challenge. Since exosome-mediated signaling showed great implications in various human diseases, this work may provide a new mechanism for PCBs-associated toxicity.

Environmental Pollution (Oxford, United Kingdom) published new progress about Disease, animal. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Recommanded Product: p-Benzenediacrylanilide, 4′,4′′-di-2-imidazolin-2-yl-, dihydrochloride.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem