Month: October 2022

Won, Jong Hoon; Kim, Seok Kyun; Shin, In Chul; Ha, Hae Chan; Jang, Ji Min; Back, Moon Jung; Kim, Dae Kyong published the artcile< Dopamine transporter trafficking is regulated by neutral sphingomyelinase 2/ceramide kinase>, Recommanded Product: p-Benzenediacrylanilide, 4′,4′′-di-2-imidazolin-2-yl-, dihydrochloride, the main research area is dopamine ceramide kinase SMase2 C1P CERK signaling cortex pheochromocytoma; Ceramide; Ceramide-1-phosphate; Dopamine transporter; Neutral sphingomyelinase 2; Sphingomyelin pathway; Trafficking.

Dopamine (DA) reuptake is the primary mechanism to terminate dopaminergic transmission in the synaptic cleft. The dopamine transporter (DAT) has an important role in the regulation of DA reuptake. This study provides anatomical and physiol. evidence that DAT recycling is regulated by ceramide kinase via the sphingomyelin pathway. First, the results show that DAT and neutral sphingomyelinase 2 (nSMase2) were successfully co-precipitated from striatal samples and were colocalized in the mouse striatum or PC12 cells. We also identified a protein-protein interaction between nSMase2 and DAT through in situ proximity ligation assay experiments in the mouse striatum. Second, dopamine (DA) stimulated the formation of ceramide and increased nSMase activity in PC12 cells, while treatment with a cell-permeable ceramide-1-phosphate (C1P) increased DA uptake. Third, we used inhibitors and siRNA to inhibit nSMase2 and ceramide kinase and observed the effects on DAT recycling in PC12 cells. Treatment with ceramide kinase inhibitor K1, or nSMase inhibitor GW4869, decreased DA uptake in PC12 cells, although the application of FB1, a ceramide synthase inhibitor, did not affect DA uptake. Transfection of nSMase2 and CERK siRNA decreased DAT surface level in PC12 cells. These results suggested that SM-derived C1P affects cell surface levels of DAT.

Cellular Signalling published new progress about Brain corpus striatum, dorsal striatum Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Recommanded Product: p-Benzenediacrylanilide, 4′,4′′-di-2-imidazolin-2-yl-, dihydrochloride.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Kusama, Hitoshi; Arakawa, Hironori; Sugihara, Hideki published the artcile< Density functional study of imidazole-iodine interaction and its implication in dye-sensitized solar cell>, SDS of cas: 1003-21-0, the main research area is imidazole iodine interaction dye sensitized solar cell; charge transfer complex imidazole iodine density functional theory.

The monomer and charge-transfer complexes of 14 different imidazole derivatives with diiodine were studied by a d. functional theory (DFT) method. DFT calculations revealed that the σ* orbital of iodine interacts with the nitrogen lone pair of imidazoles at position 3. The influence of these imidazoles addition on the performance of a bis(tetrabutylammonium)cis-bis(thiocyanato)bis(2,2′-bipyridine-4-carboxylic acid, 4′-carboxylate)ruthenium(II) (N719) dye-sensitized nanocrystalline TiO2 solar cell with an I-/I3- redox electrolyte in acetonitrile was also studied. All of the imidazole derivatives enhanced the open-circuit photovoltage (Voc). The resulting Voc values of solar cell were compared to computational calculations on the interaction between imidazoles and I2 by a DFT method. Optimized geometries, frequency analyses, and interaction energies suggest that the V oc value is higher, the more the imidazole complexes with I2.

Journal of Photochemistry and Photobiology, A: Chemistry published new progress about Bond angle. 1003-21-0 belongs to class imidazoles-derivatives, and the molecular formula is C4H5BrN2, SDS of cas: 1003-21-0.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Akopyan, A. V.; Eseva, E. A.; Polikarpova, P. D.; Baigil’diev, T. M.; Rodin, I. A.; Anishnov, A. V. published the artcile< Catalytic Activity of Polyfunctional Ionic Liquids in Oxidation of Model Sulfur Organic Compounds>, Computed Properties of 700370-07-6, the main research area is imidazolium molybdate ionic liquid oxidative desulfurization catalyst.

Ionic liquids based on 1-methylimidazole were synthesized. The liquids contain Bronsted acid centers in the cation and a transition metal atom in the anion. The polyfunctional ionic liquids synthesized in the study are effective catalysts for the oxidative desulfurization process. The conditions are found for reaching the 100% conversion of Me Ph sulfide under mild conditions in the presence of the catalysts, ionic liquids [ionic liquid: 3-(carboxymethyl)-1-methyl-1H-imidazol-3-ium molybdate with S:Mo molar ratio = 24:1, 2 h, 40°C, H2O2:S molar ratio = 12:1].

Russian Journal of Applied Chemistry published new progress about Anion exchange (with molybdate, tungstate and vanadate). 700370-07-6 belongs to class imidazoles-derivatives, and the molecular formula is C6H9ClN2O2, Computed Properties of 700370-07-6.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Taylor, Steven J.; Abeywardane, Asitha; Liang, Shuang; Muegge, Ingo; Padyana, Anil K.; Xiong, Zhaoming; Hill-Drzewi, Melissa; Farmer, Bennett; Li, Xiang; Collins, Brandon; Li, John Xiang; Heim-Riether, Alexander; Proudfoot, John; Zhang, Qiang; Goldberg, Daniel; Zuvela-Jelaska, Ljiljana; Zaher, Hani; Li, Jun; Farrow, Neil A. published the artcile< Fragment-Based Discovery of Indole Inhibitors of Matrix Metalloproteinase-13>, Recommanded Product: 5-Bromo-1-methyl-1H-imidazole, the main research area is indole derivative structure MMP 13 inhibitor arthritis.

Matrix metalloproteases (MMPs) play an important role in cartilage homeostasis under both normal and inflamed disease states and, thus, have become attractive targets for the treatment of arthritic diseases. Herein, we describe the identification of a potent, selective MMP-13 inhibitor, developed using fragment-based structure-guided lead identification and optimization techniques. Virtual screening methods identified a novel, indole-based MMP-13 inhibitor that bound into the S1′ pocket of the protein exhibiting a novel interaction pattern hitherto not observed in MMP-13 inhibitors. X-ray crystallog. structures were used to guide the elaboration of the fragment, ultimately leading to a potent inhibitor that was >100-fold selective over nine other MMP isoforms tested.

Journal of Medicinal Chemistry published new progress about Antiarthritics. 1003-21-0 belongs to class imidazoles-derivatives, and the molecular formula is C4H5BrN2, Recommanded Product: 5-Bromo-1-methyl-1H-imidazole.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Widom, Julia R.; Hoeher, Janson E. published the artcile< Base-Stacking Heterogeneity in RNA Resolved by Fluorescence-Detected Circular Dichroism Spectroscopy>, Application In Synthesis of 452-06-2, the main research area is .

RNA plays a critical role in many biol. processes, and the structures it adopts are intimately linked to those functions. Among many factors that contribute to RNA folding, van der Waals interactions between adjacent nucleobases stabilize structures in which the bases are stacked on top of one another. Here, we utilize fluorescence-detected CD spectroscopy (FDCD) to investigate base-stacking heterogeneity in RNA labeled with the fluorescent adenine analog 2-aminopurine (2-AP). Comparison of standard (transmission-detected) CD and FDCD spectra reveals that in dinucleotides, 2-AP fluorescence is emitted almost exclusively by unstacked mols. In a trinucleotide, some fluorescence is emitted by a population of stacked and highly quenched mols., but more than half originates from a minor ~10% population of unstacked mols. The combination of FDCD and standard CD measurements reveals the prevalence of stacked and unstacked conformational subpopulations as well as their relative fluorescence quantum yields.

Journal of Physical Chemistry Letters published new progress about 452-06-2. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Application In Synthesis of 452-06-2.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Dong, Jianghong; Chen, Shengwei; Li, Runfeng; Cui, Wei; Jiang, Haiming; Ling, Yixia; Yang, Zifeng; Hu, Wenhui published the artcile< Imidazole-based pinanamine derivatives: Discovery of dual inhibitors of the wild-type and drug-resistant mutant of the influenza A virus>, Related Products of 36947-69-0, the main research area is imidazole pinanamine antiviral influenza virus; Dual inhibitory activity; Influenza A virus; M2 ion channel; Pinanamine derivatives.

The authors previously reported potent hit compound (I) inhibiting the wild-type influenza A virus A/HK/68 (H3N2) and A/M2-S31N mutant viruses A/WS/33 (H1N1), with its latter activity quite weak. To further increase its potency, a structure-activity relationship study of a series of imidazole-linked pinanamine derivatives was conducted by modifying the imidazole ring of this compound Several compounds of this series inhibited the amantadine-sensitive virus at low micromolar concentrations Among them, (II) (R1,R2,R3=alkyl) was the most potent compound, which was identified as being active on an amantadine-sensitive virus through blocking of the viral M2 ion channel. Furthermore, II markedly inhibited the amantadine-resistant virus (IC50 = 3.4 μM) and its activity increased by almost 24-fold compared to initial compound, with its action mechanism being not M2 channel mediated.

European Journal of Medicinal Chemistry published new progress about Antiviral agents. 36947-69-0 belongs to class imidazoles-derivatives, and the molecular formula is C7H12N2, Related Products of 36947-69-0.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Marshall, Checkers R.; Dvorak, Josh P.; Twight, Liam P.; Chen, Lan; Kadota, Kentaro; Andreeva, Anastasia B.; Overland, Alexandra E.; Ericson, Thomas; Cozzolino, Anthony F.; Brozek, Carl K. published the artcile< Size-Dependent Properties of Solution-Processable Conductive MOF Nanocrystals>, Computed Properties of 1003-21-0, the main research area is conductive iron triazolate MOF nanocrystal size property.

The diverse optical, magnetic, and electronic behaviors of most colloidal semiconductor nanocrystals emerge from materials with limited structural and elemental compositions Conductive metal-organic frameworks (MOFs) possess rich compositions with complex architectures but remain unexplored as nanocrystals, hindering their incorporation into scalable devices. Here, we report the controllable synthesis of conductive MOF nanoparticles based on Fe(1,2,3-triazolate)2. Sizes can be tuned to as small as 5.5 nm, ensuring indefinite colloidal stability. These solution-processable MOFs can be analyzed by solution-state spectroscopy and electrochem. and cast into conductive thin films with excellent uniformity. This unprecedented anal. of MOF materials reveals a strong size dependence in optical and electronic behaviors sensitive to the intrinsic porosity and guest-host interactions of MOFs. These results provide a radical departure from typical MOF characterization, enabling insights into phys. properties otherwise impossible with bulk analogs while offering a roadmap for the future of MOF nanoparticle synthesis and device fabrication.

Journal of the American Chemical Society published new progress about Absorptivity. 1003-21-0 belongs to class imidazoles-derivatives, and the molecular formula is C4H5BrN2, Computed Properties of 1003-21-0.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Vora, Ashish; Zhou, Wenshuo; Londono-Renteria, Berlin; Woodson, Michael; Sherman, Michael B.; Colpitts, Tonya M.; Neelakanta, Girish; Sultana, Hameeda published the artcile< Arthropod EVs mediate dengue virus transmission through interaction with a tetraspanin domain containing glycoprotein Tsp29Fb>, Computed Properties of 6823-69-4, the main research area is Tsp29Fb E protein dengue virus transmission extracellular vesicle Aedes; HSP70; Tsp29Fb; arthropod EVs; dengue; transmission.

Dengue virus (DENV) is a mosquito-borne flavivirus that causes dengue fever in humans, worldwide. Using in vitro cell lines derived from Aedes albopictus and Aedes aegypti, the primary vectors of DENV, we report that DENV2/DENV3-infected cells secrete extracellular vesicles (EVs), including exosomes, containing infectious viral RNA and proteins. A full-length DENV2 genome, detected in arthropod EVs, was infectious to naive mosquito and mammalian cells, including human-skin keratinocytes and blood endothelial cells. Cryo-electron microscopy showed mosquito EVs with a size range from 30 to 250 nm. Treatments with RNase A, Triton X-100, and 4G2 antibody-bead binding assays showed that infectious DENV2-RNA and proteins are contained inside EVs. Viral plaque formation and dilution assays also showed securely contained infectious viral RNA and proteins in EVs are transmitted to human cells. Up-regulated HSP70 upon DENV2 infection showed no role in viral replication and transmission through EVs. In addition, qRT-PCR and immunoblotting results revealed that DENV2 up-regulates expression of a mosquito tetraspanin-domain-containing glycoprotein, designated as Tsp29Fb, in A. aegypti mosquitoes, cells, and EVs. RNAi-mediated silencing and antibody blocking of Tsp29Fb resulted in reduced DENV2 loads in both mosquito cells and EVs. Immunoprecipitation showed Tsp29Fb to directly interact with DENV2 E-protein. Furthermore, treatment with GW4869 (exosome-release inhibitor) affected viral burden, direct interaction of Tsp29Fb with E-protein and EV-mediated transmission of viral RNA and proteins to naive human cells. In summary, we report a very important finding on EV-mediated transmission of DENV2 from arthropod to mammalian cells through interactions with an arthropod EVs-enriched marker Tsp29Fb.

Proceedings of the National Academy of Sciences of the United States of America published new progress about Aedes aegypti. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Computed Properties of 6823-69-4.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

He, Yanping; Chang, Yangyang; Chen, Da; Liu, Juewen published the artcile< Probing Local Folding Allows Robust Metal Sensing Based on a Na+-Specific DNAzyme>, Application of C5H5N5, the main research area is sodium DNAzyme metal sensing probing; DNA; aptamers; biosensors; fluorescence; sodium.

Fluorescent metal sensors based on DNA often rely on changes in end-to-end distance or local environmental near fluorophore labels. Because metal ions can also nonspecifically interact with DNA through various mechanisms, such as charge screening, base binding, and increase or decrease in duplex stability, robust and specific sensing of metal ions has been quite challenging. In this work, a side-by-side comparison of two signaling strategies on a Na+-specific DNAzyme that contained a Na+-binding aptamer was performed. The duplex regions of the DNAzyme was systematically shortened and its effect was studied by using a 2-aminopurine (2AP)-labeled substrate strand. Na+ binding affected the local environmental of the 2AP label and increased its fluorescence. A synergistic process of Na+ binding and forming the duplex on the 5′-end of the enzyme strand was observed, and this end was close to the aptamer loop. The effect on the 3′-end is more continuous, and the stem needs to form first before Na+ can bind. With an optimized substrate binding arm, a FRET-based sensor has been designed by labeling the two ends of a cis form of the DNAzyme with two fluorophores. In this case, Na+ failed to show a distinct difference from that of Li+ or K+; thus indicating that probing changes to the local environment allows more robust sensing of metal ions.

ChemBioChem published new progress about Aptamers. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Application of C5H5N5.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Kummer, David A.; Cummings, Maxwell D.; Abad, Marta; Barbay, Joseph; Castro, Glenda; Wolin, Ronald; Kreutter, Kevin D.; Maharoof, Umar; Milligan, Cynthia; Nishimura, Rachel; Pierce, Joan; Schalk-Hihi, Celine; Spurlino, John; Urbanski, Maud; Venkatesan, Hariharan; Wang, Aihua; Woods, Craig; Xue, Xiaohua; Edwards, James P.; Fourie, Anne M.; Leonard, Kristi published the artcile< Identification and structure activity relationships of quinoline tertiary alcohol modulators of RORγt>, Computed Properties of 1003-21-0, the main research area is quinoline tertiary alc preparation RORgammat antagonist inverse agonist; Agonist; IL-17; Inverse agonist; Neutral antagonist; RORγt; Retinoic acid-related orphan nuclear receptor gamma t; Th17.

A high-throughput screen of the ligand binding domain of the nuclear receptor retinoic acid-related orphan receptor gamma t (RORγt) employing a thermal shift assay yielded a quinoline tertiary alc. hit. Optimization of the 2-, 3- and 4-positions of the quinoline core using structure-activity relationships and structure-based drug design methods led to the discovery of a series of modulators with improved RORγt inhibitory potency and inverse agonism properties.

Bioorganic & Medicinal Chemistry Letters published new progress about Drug design (structure-based). 1003-21-0 belongs to class imidazoles-derivatives, and the molecular formula is C4H5BrN2, Computed Properties of 1003-21-0.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem