Maciejewska, Agnieszka M.; Poznanski, Jaroslaw; Kaczmarska, Zuzanna; Krowisz, Beata; Nieminuszczy, Jadwiga; Polkowska-Nowakowska, Agnieszka; Grzesiuk, Elzbieta; Kusmierek, Jaroslaw T. published an article in 2013, the title of the article was AlkB Dioxygenase Preferentially Repairs Protonated Substrates.Synthetic Route of 55662-66-3 And the article contains the following content:
Efficient repair by Escherichia coli AlkB dioxygenase of exocyclic DNA adducts 3,N4-ethenocytosine, 1,N6-ethenoadenine, 3,N4-α-hydroxyethanocytosine, and reported here for the first time 3,N4-α-hydroxypropanocytosine, requires higher Fe(II) concentration than the reference 3-methylcytosine. The pH optimum for the repair follows the order of pKa values for protonation of the adduct, suggesting that pos. charged substrates favorably interact with the neg. charged carboxylic group of Asp-135 side chain in the enzyme active center. This interaction is supported by mol. modeling, indicating that 1,N6-ethenoadenine and 3,N4-ethenocytosine are bound to AlkB more favorably in their protonated cationic forms. An anal. of the pattern of intermol. interactions that stabilize the location of the ligand points to a role of Asp-135 in recognition of the adduct in its protonated form. Moreover, ab initio calculations also underline the role of substrate protonation in lowering the free energy barrier of the transition state of epoxidation of the etheno adducts studied. The observed time courses of repair of mixtures of stereoisomers of 3,N4-α-hydroxyethanocytosine or 3,N4-α-hydroxypropanocytosine are unequivocally two-exponential curves, indicating that the resp. isomers are repaired by AlkB with different efficiencies. Mol. modeling of these adducts bound by AlkB allowed evaluation of the participation of their possible conformational states in the enzymic reaction. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Synthetic Route of 55662-66-3
The Article related to alkb dioxygenase recognition exocyclic dna adduct protonation, Enzymes: Substrates-Cofactors-Inhibitors-Activators-Coenzymes-Products and other aspects.Synthetic Route of 55662-66-3
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem