On January 31, 1989, Eberle, Gertrud; Barbin, Alain; Laib, Reinhold J.; Ciroussel, Francoise; Thomale, Juergen; Bartsch, Helmut; Rajewsky, Manfred F. published an article.Safety of Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was 1,N6-etheno-2′-deoxyadenosine and 3,N4-etheno-2′-deoxycytidine detected by monoclonal antibodies in lung and liver DNA of rats exposed to vinyl chloride. And the article contained the following:
1,N6-Etheno-2′-deoxyadenosine (εdAdo) and 3,N4-etheno-2′-deoxycytidine (εdCyd) are formed in vitro by reaction of DNA with the electrophilic metabolites of vinyl chloride (VC), chloroethylene oxide and chloroacetaldehyde. To detect and quantitate these DNA adducts in vivo, a series of specific monoclonal antibodies (Mab) were raised. Among those, Mab EM-A-1 and Mab EM-C-1, resp., were used for detection of εdAdo and εdCyd by competitive RIA, following preseparation of the etheno adducts from DNA hydrolyzates by HPLC. At 50% inhibition of tracer-antibody binding, both Mab had a detection limit of 187 fmol and antibody affinity constants (K) of 2 × 109 L/mol. The levels of εdAdo and εdCyd were quantitated in the DNA of lung and liver tissue of young rats exposed to 2000 ppm of VC for 10 days. The εdAdo/2′-deoxyadenosine and εdCyd/2′-deoxycytidine molar ratios were 1.3 × 10-7 and 3.3 × 10-7, resp., in lung DNA, and 5.0 × 10-8 and 1.6 × 10-7 in liver DNA. When hydrolyzates of 3 mg of DNA were analyzed by RIA at 25% inhibition of tracer-antibody binding, εdAdo and εdCyd were not detected in liver DNA from untreated rats above the limiting εdAdo/2′-deoxyadenosine and εdCyd/2′-deoxycytidine molar ratios of 2.2 × 10-8 and 3.1 × 10-8, resp. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Safety of Imidazo[1,2-c]pyrimidin-5(6H)-one
The Article related to vinyl chloride ethenodeoxyadenosine ethenodeoxycytidine dna, lung dna adduct vinyl chloride, liver dna adduct vinyl chloride, Toxicology: Carcinogens, Mutagens, and Teratogens and other aspects.Safety of Imidazo[1,2-c]pyrimidin-5(6H)-one
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem