On April 23, 2004, Gros, Laurent; Maksimenko, Andrei V.; Privezentzev, Cyril V.; Laval, Jacques; Saparbaev, Murat K. published an article.Name: Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N4-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct. And the article contained the following:
Lipid peroxidation generates aldehydes, which react with DNA bases, forming genotoxic exocyclic etheno(ε)-adducts. E-bases have been implicated in vinyl chloride-induced carcinogenesis, and increased levels of these DNA lesions formed by endogenous processes are found in human degenerative disorders. E-adducts are repaired by the base excision repair pathway. Here, the authors report the efficient biol. hijacking of the human alkyl-N-purine-DNA glycosylase (ANPG) by 3,N4-ethenocytosine (εC) when present in DNA. Unlike the ethenopurines, ANPG does not excise, but binds to εC when present in either double-stranded or single-stranded DNA. The authors developed a direct assay, based on the fluorescence quenching mechanism of mol. beacons, to measure a DNA glycosylase activity. Mol. beacons containing modified residues have been used to demonstrate that the εC·ANPG interaction inhibits excision repair both in reconstituted systems and in cultured human cells. Furthermore, the authors show that the εC·ANPG complex blocks primer extension by the Klenow fragment of DNA polymerase I. These results suggest that εC could be more genotoxic than 1,N6-ethenoadenine (εA) residues in vivo. The proposed model of ANPG-mediated genotoxicity of εC provides a new insight in the mol. basis of lipid peroxidation-induced cell death and genome instability in cancer. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Name: Imidazo[1,2-c]pyrimidin-5(6H)-one
The Article related to genotoxicity ethenocytosine lipid peroxidation dna adduct human anpg, Toxicology: Carcinogens, Mutagens, and Teratogens and other aspects.Name: Imidazo[1,2-c]pyrimidin-5(6H)-one
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem