On December 31, 2001, Chen, Hauh-Jyun Candy; Lin, Tai-Chun; Hong, Chia-Liang; Chiang, Li-Chang published an article.Formula: C6H5N3O The title of the article was Analysis of 3,N4-Ethenocytosine in DNA and in Human Urine by Isotope Dilution Gas Chromatography/Negative Ion Chemical Ionization/Mass Spectrometry. And the article contained the following:
The promutagenic etheno DNA adducts have been detected in tissue DNA of rodents and humans from various exogenous and endogenous sources. While other etheno DNA adducts have been detected and quantified by isotope dilution gas chromatog./neg. ion chem. ionization/mass spectrometry (GC/NICI/MS), similar anal. for 3,N4-ethenocytosine (εCyt) has not been available. In this report, a GC/NICI/MS assay was developed for detection and quantification of εCyt in DNA and in human urine samples. The stable isotope of εCyt with 7 mass units higher than the normal εCyt was synthesized and used as internal standard of the assay. The adduct-enriched fraction of DNA hydrolyzate was derivatized with pentafluorobenzyl bromide before GC/NICI/MS anal. with selective ion monitoring at [M – 181]- fragments of pentafluorobenzylated εCyt and its isotope analog. One femtogram (S/N > 40) of pentafluorobenzylated εCyt was detected when injected on column with selective ion monitoring mode. The limit of quantification for the entire assay was 7.4 fmol of εCyt, which was approx. one thousand times lower than that of the HPLC/fluorescence assay for the nucleoside 3,N4-etheno-2′-deoxycytidine in DNA. Anal. of chloroacetaldehyde-treated calf thymus DNA by both GC/NICI/MS and HPLC/fluorescence methods provided similar adduct levels and thus verified the assay. This GC/NICI/MS method was used for anal. of εCyt in two smokers’ urine samples and the average level of εCyt was 101±17 pg/mL/g of creatinine. Thus, quantification of Cyt in DNA and in urine by this highly specific and ultrasensitive isotope dilution GC/NICI/MS assay may facilitate research on the role of εCyt in carcinogenesis and in cancer development. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Formula: C6H5N3O
The Article related to ethenocytosine dna adduct human urine isotope dilution gc ms, Toxicology: Methods (Including Analysis) and other aspects.Formula: C6H5N3O
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem