On January 31, 2021, Liu, Xiaoling; Lai, Weiyi; Li, Yao; Chen, Shaokun; Liu, Baodong; Zhang, Ning; Mo, Jiezhen; Cong, Lyu; Zheng, Jing; Du, Ya-Rui; Jiang, Guibin; Xu, Guo-Liang; Wang, Hailin published an article.Recommanded Product: N-Methyl-7H-purin-6-amine The title of the article was N6-methyladenine is incorporated into mammalian genome by DNA polymerase. And the article contained the following:
AIM: This article discussed about incorporation of N6-methyladenine into mammalian genome by DNA polymerase. To provide robust and reliable data, contamination-free UHPLC-MS/MS technol. was for ultrasensitive and accurate detection of N6-methyladenine in mammals. We measured N6-methyladenine in human embryonic kidney cells, mesenchymal stem cells and embryonic stem cells. Similar levels of DNA 6mA were detected by treating late G1-phase arresting agent L-mimosine. We observed accumulation of genomic 6mA in both mouse and human ES cells and HEK293T cells. By using early G1-phase arresting palbociclib, we observed moderate increase of N6-methyladenine. We exploited unique stable isotope-labeled deoxyadenosine tracing technol. to investigate 6mA origin. UHPLC-MS/MS anal. revealed >67% of genomic deoxyadenosine was labeled in form of [15N4]-deoxyadenosine. Despite efficient labeling of dA, we failed to detect any [15N4]-6mA in three tested cell lines at all cell cycle phases. We observed [15N4]-6mA by transfecting HEK293T cells with plasmid carrying E. coli 6mA methylase mutant gene has activity of 100 times lower than wild type. We used second stable isotope labeling reagent [13CD3] L-methionine. We did not detect any [13CD3]-6mA in the treated mES cells at all cell cycle phases. Results: 6mA is independent of methylases, proving origin of methylase-independent 6mA. Conclusion: N6-methyladenine is incorporated into mammalian genome by DNA polymerase. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: N-Methyl-7H-purin-6-amine
The Article related to n6 methyladenosine dna polymerase genome embryonic kidney cell, Biochemical Genetics: Methods and other aspects.Recommanded Product: N-Methyl-7H-purin-6-amine
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem