On October 1, 1979, Slaughter, Robert S.; Barnes, Eugene M. Jr. published an article.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was Hypoxanthine transport by Chinese hamster lung fibroblasts: kinetics and inhibition by nucleosides. And the article contained the following:
The transport of 3H-labeled hypoxanthine (I) was studied in monolayer cultures of mutant Chinese hamster lung fibroblasts lacking guanine phosphoribosyltransferase. Initial rates of transport were determined by rapid uptake experiments (8-20 s); a Michaelis constant of 0.68 mM for I was derived from linear, monophasic plots of v/S against v. Nucleosides are competitive inhibitors of this process; adenosine and thymidine give resp. Ki values of 86 and 300 μM. The corresponding bases give much higher inhibition constants with adenine and thymine yielding values of 3100 and 1700 μM, resp. A similar pattern was observed for competitive inhibition of I transport by inosine, adenine arabinoside, uridine, cytidine, and 2 ribofuranosylimidazo derivatives of pyrimidin-4-one; in every case the nucleoside exhibited a lower Ki value than the corresponding homologous base. The inhibition constants observed for nucleosides are remarkably similar to their Km values for nucleoside transport by cultured cells recently reported. I transport was also blocked by the 6-(2-hydroxy-5-nitrobenzylthio) derivatives of inosine and guanosine and by dipyridamole; these agents are also inhibitors of nucleoside transport. These results indicate a closer relation between base and nucleoside transport than previously recognized and suggest that these 2 transport processes may involve identical or very similar transport proteins. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one
The Article related to hypoxanthine fibroblast transport determination, biol transport hypoxanthine isotope determination, nucleoside inhibition hypoxanthine transport, Biochemical Methods: Isotopic and other aspects.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one
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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem