Month: November 2022

Jenjaroenpun, Piroon; Wongsurawat, Thidathip; Wadley, Taylor D.; Wassenaar, Trudy M.; Liu, Jun; Dai, Qing; Wanchai, Visanu; Akel, Nisreen S.; Jamshidi-Parsian, Azemat; Franco, Aime T.; Boysen, Gunnar; Jennings, Michael L.; Ussery, David W.; He, Chuan; Nookaew, Intawat published an article in 2021, the title of the article was Decoding the epitranscriptional landscape from native RNA sequences.COA of Formula: C6H7N5 And the article contains the following content:

Traditional epitranscriptomics relies on capturing a single RNA modification by antibody or chem. treatment, combined with short-read sequencing to identify its transcriptomic location. This approach is labor-intensive and may introduce exptl. artifacts. Direct sequencing of native RNA using Oxford Nanopore Technologies (ONT) can allow for directly detecting the RNA base modifications, although these modifications might appear as sequencing errors. The percent Error of Specific Bases (%ESB) was higher for native RNA than unmodified RNA, which enabled the detection of ribonucleotide modification sites. Based on the %ESB differences, we developed a bioinformatic tool, epitranscriptional landscape inferring from glitches of ONT signals (ELIGOS), that is based on various types of synthetic modified RNA and applied to rRNA and mRNA. ELIGOS is able to accurately predict known classes of RNA methylation sites (AUC > 0.93) in rRNAs from Escherichia coli, yeast, and human cells, using either unmodified in vitro transcription RNA or a background error model, which mimics the systematic error of direct RNA sequencing as the reference The well-known DRACH/RRACH motif was localized and identified, consistent with previous studies, using differential anal. of ELIGOS to study the impact of RNA m6A methyltransferase by comparing wild type and knockouts in yeast and mouse cells. Lastly, the DRACH motif could also be identified in the mRNA of three human cell lines. The mRNA modification identified by ELIGOS is at the level of individual base resolution In summary, we have developed a bioinformatic software package to uncover native RNA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).COA of Formula: C6H7N5

The Article related to decoding epitranscriptional rna sequence, Placeholder for records without volume info and other aspects.COA of Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Mothukuri, Ganesh K.; Kale, Sangram S.; Stenbratt, Carl L.; Zorzi, Alessandro; Vesin, Jonathan; Bortoli Chapalay, Julien; Deyle, Kaycie; Turcatti, Gerardo; Cendron, Laura; Angelini, Alessandro; Heinis, Christian published an article in 2020, the title of the article was Macrocycle synthesis strategy based on step-wise “adding and reacting” three components enables screening of large combinatorial libraries.Category: imidazoles-derivatives And the article contains the following content:

Macrocycles provide an attractive modality for drug development, but generating ligands for new targets is hampered by the limited availability of large macrocycle libraries. We have established a solution-phase macrocycle synthesis strategy in which three building blocks are coupled sequentially in efficient alkylation reactions that eliminate the need for product purification We demonstrate the power of the approach by combinatorially reacting 15 bromoacetamide-activated tripeptides, 42 amines, and 6 bis-electrophile cyclization linkers to generate a 3780-compound library with minimal effort. Screening against thrombin yielded a potent and selective inhibitor (Ki = 4.2 �0.8 nM) that efficiently blocked blood coagulation in human plasma. Structure-activity relationship and X-ray crystallog. anal. revealed that two of the three building blocks acted synergistically and underscored the importance of combinatorial screening in macrocycle development. The three-component library synthesis approach is general and offers a promising avenue to generate macrocycle ligands to other targets. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Category: imidazoles-derivatives

The Article related to plasma thrombin prothrombin thromboplastin x, Placeholder for records without volume info and other aspects.Category: imidazoles-derivatives

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Liu, Dan; Xue, Aiqi; Wang, Haifeng; Luan, Tian; Li, Xue published an article in 2022, the title of the article was Design, synthesis and biological evaluation of 4-amino-quinolines as antitumor agents.Recommanded Product: N-(3-Aminopropyl)-imidazole And the article contains the following content:

Fifteen novel 4-amino-quinolines (I1-III3) as antitumor agent were synthesized by p-nitroaniline and ethoxymethylene malonic ester (EMME) via condensation, cyclization, hydrolysis, decarboxylation, chlorination, nucleophilic substitution, reduction and amidation. The antitumor activity of compounds I1-III3 was evaluated on SGC-7901, BEL-7402 and A549 cancer cell lines. In vitro bioassay indicated that some compounds showed different degree activity against all tested cancer cell lines. Compound I1, I4 and II2 exhibited high effects against A549 cell lines (IC50 = 1.34渭M, 1.36渭M and 3.00渭M, resp.). In addition, the result of mol. docking showed that compound I1, I4 and II2 could dock into the pocket of EGFR. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Recommanded Product: N-(3-Aminopropyl)-imidazole

The Article related to amino quinoline antitumor agent egfr receptor, Placeholder for records without volume info and other aspects.Recommanded Product: N-(3-Aminopropyl)-imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Woodcock, Clayton B.; Horton, John R.; Zhou, Jujun; Bedford, Mark T.; Blumenthal, Robert M.; Zhang, Xing; Cheng, Xiaodong published an article in 2020, the title of the article was Biochemical and structural basis for YTH domain of human YTHDC1 binding to methylated adenine in DNA.Name: N-Methyl-7H-purin-6-amine And the article contains the following content:

The recently characterized mammalian writer (methyltransferase) and eraser (demethylase) of the DNA N6-methyladenine (N6mA) Me mark act on single-stranded (ss) and transiently-unpaired DNA. As YTH domain-containing proteins bind N6mA-containing RNA in mammalian cells, we investigated whether mammalian YTH domains are also Me mark readers of N6mA DNA. Here, we show that the YTH domain of YTHDC1 (known to localize in the nucleus) binds ssDNA containing N6mA, with a 10 nM dissociation constant This binding is stronger by a factor of 5 than in an RNA context, tested under the same conditions. However, the YTH domains of YTHDF2 and YTHDF1 (predominantly cytoplasmic) exhibited the opposite effect with �.5-2x stronger binding to ssRNA containing N6mA than to the corresponding DNA. We determined two structures of the YTH domain of YTHDC1 in complex with N6mA-containing ssDNA, which illustrated that YTHDC1 binds the methylated adenine in a single-stranded region flanked by duplexed DNA. We discuss the hypothesis that the writer-reader-eraser of N6mA-containining ssDNA is associated with maintaining genome stability. Structural comparison of YTH and SRA domains (the latter a DNA 5-methylcytosine reader) revealed them to be diverse members of a larger family of DNA/RNA modification readers, apparently having originated from bacterial modification-dependent restriction enzymes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to ythdc1 dna methylated adenine biochem structure, Placeholder for records without volume info and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On April 20, 2022, Bai, Jie; Wang, Jie; Feng, Yecheng; Yao, Yongfang; Zhao, Xubo published an article.Electric Literature of 5036-48-6 The title of the article was Stability-tunable core-crosslinked polymeric micelles based on an imidazole-bearing block polymer for pH-responsive drug delivery. And the article contained the following:

Polymeric micelles from block copolymers have gained increasing attention in cancer drug delivery. However, the fabrication of polymeric micelles that are stable in biol. fluids and unstable at tumor sites remains a principal challenge. To achieve the tunable stability for the polymeric micelles, Zn coordination-induced core-crosslinked polymeric micelles have been proposed and confirmed. Herein, a block copolymer bearing imidazole pendants is synthesized which is capable of self-assembling into polymeric micelles in water. After core-crosslinking, it can be found that the critical micelle concentration (CMC) of the core-crosslinked polymeric micelles is significantly lower than that of non-crosslinked polymeric micelles. Particularly, the drug-loaded core-crosslinked polymeric micelles are fragile and easily affected by the slightly acidic environments, which makes it possible to turn the polymeric micelles back to hydrophilic polymers and therefore disassemble micelles to unload cargoes. The drug-loaded core-crosslinked polymeric micelles display adequate toxicity as a result of their low IC50 value of 3.713 �0.166 渭g/mL. Collectively, Zn coordination-induced core-crosslinked polymeric micelles offer a design idea for practicable drug delivery system in cancer therapy. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Electric Literature of 5036-48-6

The Article related to imidazole block polymer micelle drug delivery systems, Placeholder for records without volume info and other aspects.Electric Literature of 5036-48-6

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Zhang, Guoqiang; Xu, Yongru; Wang, Xiaona; Zhu, Yuanxiang; Wang, Liangliang; Zhang, Wenxin; Wang, Yiru; Gao, Yajie; Wu, Xuna; Cheng, Ying; Sun, Qinmiao; Chen, Dahua published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay. And the article contained the following:

Maternal RNA degradation is critical for embryogenesis and is tightly controlled by maternal RNA-binding proteins. Fragile X mental-retardation protein (FMR1) binds target mRNAs to form ribonucleoprotein (RNP) complexes/granules that control various biol. processes, including early embryogenesis. However, how FMR1 recognizes target mRNAs and how FMR1-RNP granule assembly/disassembly regulates FMR1-associated mRNAs remain elusive. Here we show that Drosophila FMR1 preferentially binds mRNAs containing m6A-marked “AGACU” motif with high affinity to contributes to maternal RNA degradation The high-affinity binding largely depends on a hydrophobic network within FMR1 KH2 domain. Importantly, this binding greatly induces FMR1 granule condensation to efficiently recruit unmodified mRNAs. The degradation of maternal mRNAs then causes granule de-condensation, allowing normal embryogenesis. Our findings reveal that sequence-specific mRNAs instruct FMR1-RNP granules to undergo a dynamic phase-switch, thus contributes to maternal mRNA decay. This mechanism may represent a general principle that regulated RNP-granules control RNA processing and normal development. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to methyladenosine modification fmr phase switch rna decay, Placeholder for records without volume info and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem