Month: November 2022

On October 31, 2021, Li, Xuwen; Zhang, Zijian; Luo, Xinlong; Schrier, Jacob; Yang, Andrew D.; Wu, Tao P. published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was The exploration of N6-deoxyadenosine methylation in mammalian genomes. And the article contained the following:

A review. N6-methyladenine (N6-mA, m6dA, or 6mA), a prevalent DNA modification in prokaryotes, has recently been identified in higher eukaryotes, including mammals. Although 6mA has been well-studied in prokaryotes, the function and regulatory mechanism of 6mA in eukaryotes are still poorly understood. Recent studies indicate that 6mA can serve as an epigenetic mark and play critical roles in various biol. processes, from transposable-element suppression to environmental stress response. Here, we review the significant advances in methodol. for 6mA detection and major progress in understanding the regulation and function of this non-canonical DNA methylation in eukaryotes, predominantly mammals. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to review dna methylation mammalian genome, dna n6-methyladenine (6ma), mammalian dna modification, non-canonical mammalian dna methylation, Biochemical Genetics: Reviews and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On November 30, 2021, Lv, Hao; Dao, Fu-Ying; Zhang, Dan; Yang, Hui; Lin, Hao published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was Advances in mapping the epigenetic modifications of 5-methylcytosine (5mC), N6-methyladenine (6mA), and N4-methylcytosine (4mC). And the article contained the following:

A review. DNA modification plays a pivotal role in regulating gene expression in cell development. As prevalent markers on DNA, 5-methylcytosine (5mC), N6-methyladenine (6mA), and N4-methylcytosine (4mC) can be recognized by specific methyltransferases, facilitating cellular defense and the versatile regulation of gene expression in eukaryotes and prokaryotes. Recent advances in DNA sequencing technol. have permitted the positions of different modifications to be resolved at the genome-wide scale, which has led to the discovery of several novel insights into the complexity and functions of multiple methylations. In this review, we summarize differences in the various mapping approaches and discuss their pros and cons with respect to their relative read depths, speeds, and costs. We also discuss the development of future sequencing technologies and strategies for improving the detection resolution of current sequencing technologies. Lastly, we speculate on the potentially instrumental role that these sequencing technologies might play in future research. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to review methylcytosine methyladenine epigenetic modification, 5-methylcytosine, dna sequencing technologies, n4-methylcytosine, n6-methyladenine, Biochemical Genetics: Reviews and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Lowe, Paige; Olinski, Ryszard; Ruzov, Alexey published an article in 2021, the title of the article was Evidence for Noncytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overview.Name: N-Methyl-7H-purin-6-amine And the article contains the following content:

A review. Cytosine DNA methylation (5-methylcytsone, 5mC) is the major DNA modification found in the genomes of animals and plants. Although the roles of 5mC and its oxidized derivatives in the regulation of gene expression are relatively well attested and extensively explored, a number of recent studies imply that noncytosine DNA modifications may also convey specific biol. functions and act as “epigenetic” marks in multicellular organisms. Here we review exptl. evidence for the presence of noncytosine epigenetic modifications in metazoans and plants focusing on two “unusual” DNA bases, 5-hydroxymethyluracil (5hmU) and N6-methyladenine (6mA), and suggest potential explanations for inconsistencies in the currently available data on abundance and potential biol. roles of these DNA modifications in mammals. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to review multicellular eukaryote noncytosine epigenetic dna modification, 5-hydroxymethyluracil, dna modifications, epigenetics, n6-methyladenine, Biochemical Genetics: Reviews and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On October 1, 1979, Slaughter, Robert S.; Barnes, Eugene M. Jr. published an article.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was Hypoxanthine transport by Chinese hamster lung fibroblasts: kinetics and inhibition by nucleosides. And the article contained the following:

The transport of 3H-labeled hypoxanthine (I) was studied in monolayer cultures of mutant Chinese hamster lung fibroblasts lacking guanine phosphoribosyltransferase. Initial rates of transport were determined by rapid uptake experiments (8-20 s); a Michaelis constant of 0.68 mM for I was derived from linear, monophasic plots of v/S against v. Nucleosides are competitive inhibitors of this process; adenosine and thymidine give resp. Ki values of 86 and 300 μM. The corresponding bases give much higher inhibition constants with adenine and thymine yielding values of 3100 and 1700 μM, resp. A similar pattern was observed for competitive inhibition of I transport by inosine, adenine arabinoside, uridine, cytidine, and 2 ribofuranosylimidazo derivatives of pyrimidin-4-one; in every case the nucleoside exhibited a lower Ki value than the corresponding homologous base. The inhibition constants observed for nucleosides are remarkably similar to their Km values for nucleoside transport by cultured cells recently reported. I transport was also blocked by the 6-(2-hydroxy-5-nitrobenzylthio) derivatives of inosine and guanosine and by dipyridamole; these agents are also inhibitors of nucleoside transport. These results indicate a closer relation between base and nucleoside transport than previously recognized and suggest that these 2 transport processes may involve identical or very similar transport proteins. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one

The Article related to hypoxanthine fibroblast transport determination, biol transport hypoxanthine isotope determination, nucleoside inhibition hypoxanthine transport, Biochemical Methods: Isotopic and other aspects.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Li, Xuwen; Guo, Shiyuan; Cui, Yan; Zhang, Zijian; Luo, Xinlong; Angelova, Margarita T.; Landweber, Laura F.; Wang, Yinsheng; Wu, Tao P. published an article.Synthetic Route of 443-72-1 The title of the article was NT-seq: a chemical-based sequencing method for genomic methylome profiling. And the article contained the following:

Abstract: DNA methylation plays vital roles in both prokaryotes and eukaryotes. There are three forms of DNA methylation in prokaryotes: N6-methyladenine (6mA), N4-methylcytosine (4mC), and 5-methylcytosine (5mC). Although many sequencing methods have been developed to sequence specific types of methylation, few technologies can be used for efficiently mapping multiple types of methylation. Here, we present NT-seq for mapping all three types of methylation simultaneously. NT-seq reliably detects all known methylation motifs in two bacterial genomes and can be used for identifying de novo methylation motifs. NT-seq provides a simple and efficient solution for detecting multiple types of DNA methylation. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to escherichia helicobacter nt seq genome methylome dna methylation, dna methylation, next-generation sequencing, whole-genome epigenetic profiling, Biochemical Genetics: Methods and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Bochtler, Matthias; Fernandes, Humberto published an article in 2021, the title of the article was DNA adenine methylation in eukaryotes: Enzymatic mark or a form of DNA damage.SDS of cas: 443-72-1 And the article contains the following content:

A Review. 6-Methyladenine (6mA) is fairly abundant in nuclear DNA of basal fungi, ciliates and green algae. In these organisms, 6mA is maintained near transcription start sites in ApT context by a parental-strand instruction dependent maintenance methyltransferase and is pos. associated with transcription. In animals and plants, 6mA levels are high only in organellar DNA. The 6mA levels in nuclear DNA are very low. They are attributable to nucleotide salvage and the activity of otherwise mitochondrial METTL4, and may be considered as a price that cells pay for adenine methylation in RNA and/or organellar DNA. Cells minimize this price by sanitizing dNTP pools to limit 6mA incorporation, and by converting 6mA that has been incorporated into DNA back to adenine. Hence, 6mA in nuclear DNA should be described as an epigenetic mark only in basal fungi, ciliates and green algae, but not in animals and plants. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to review dna adenine methylation eukaryotes, 6ma, dna damage, dna modifications, cancer, epitranscriptome/epigenome, nucleotide salvage, transcription, Biochemical Genetics: Reviews and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 1, 2020, Wu, Kou-Juey published an article.Computed Properties of 443-72-1 The title of the article was The epigenetic roles of DNA N6-Methyladenine (6mA) modification in eukaryotes. And the article contained the following:

A review. The DNA N6-methyladenine (6mA) modification is a prevalent epigenetic mark in prokaryotes, but the low abundance of 6mA in eukaryotes has recently received attention. The possible role of 6mA as an epigenetic mark in eukaryotes is starting to be recognized. This review article addresses the epigenetic roles of 6mA in eukaryotes. The existence of 6mA in metazoans and plants, the correlation of 6mA with gene expression, the enzymes catalyzing the deposition and removal of the 6mA modification, the relationship of 6mA to nucleosome positioning, the 6mA interaction with chromatin, its role in tumorigenesis and other physiol. conditions/diseases and tech. issues in 6mA detection/profiling and bioinformatics anal. are described. New directions and unresolved issues (e.g., the base-pair-resolution 6mA-sequencing method and gene activation vs. repression) in 6mA research are discussed. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Computed Properties of 443-72-1

The Article related to review epigenetic mark n6methyladenine tumorigenesis, chromatin, demethylase, epigenetic mark, gene expression, methyltransferase, nucleosome positioning, Biochemical Genetics: Reviews and other aspects.Computed Properties of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On February 11, 2021, Pennington, Lewis D.; Woods, James R.; Huynh, Hoan; Aquila, Brian M.; Mugge, Ingo Andreas; Hu, Yuan; Choi, Younggi; Wynn, Thomas Andrew; Gerard, Baudouin; Bosanac, Todd; Vantangoli, Nicholas J. published a patent.Application of 40644-16-4 The title of the patent was Lactam-containing compounds for the treatment of pain. And the patent contained the following:

Provided herein are compounds I (R1= (halogen-substituted) C1-6 alkyl, C1-4 alkoxy; R2, R3, R4= H, (halogen-substituted) C1-4 alkyl, C1-4 alkoxy, OH, halogen; R5= H, C1-4 alkyl; X and R6 as defined in text) that are useful in the treatment of pain in a subject. Also provided herein is a pharmaceutical composition comprising compounds or pharmaceutically acceptable salts thereof, and a pharmaceutically acceptable carrier and methods of treating pain in a subject in need thereof. The experimental process involved the reaction of 4-Bromo-1H-benzo[d]imidazol-2(3H)-one(cas: 40644-16-4).Application of 40644-16-4

The Article related to pharmaceutical composition pain, Pharmaceuticals: Pharmaceutics and other aspects.Application of 40644-16-4

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Nalwade, Santaji Uttam; Reddy, Vangala Ranga; Rao, Dantu Durga; Morisetti, Nagendra kumar published an article in 2011, the title of the article was A validated stability indicating ultra performance liquid chromatographic method for determination of impurities in Esomeprazole magnesium gastro resistant tablets.Safety of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole And the article contains the following content:

A novel gradient reversed-phase ultra performance liquid chromatog. method has been developed for quant. determination of Esomeprazole magnesium and its seven impurities in pharmaceutical dosage forms. Chromatog. separation has been achieved on an Acquity BEH C18, 50 mm × 2.1 mm, 1.7 μm with buffered mobile phase consisting solvent A (0.04 M (M) glycine (pH 9.0) buffer) and solvent B (mixture of acetonitrile and Milli-Q water in the ratio 90: 10 (volume/volume); resp.) delivered at flow rate of 0.21 mL min-1 and the detection wavelength 305 nm. Resolution of Esomeprazole magnesium and all the seven potential impurities has been achieved greater than 2.0 for all pairs of compounds The drug was subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation Esomeprazole magnesium was found to degrade significantly in oxidative and acid hydrolysis stress conditions and stable in base, hydrolytic and photolytic degradation conditions. The degradation products were well resolved from main peak and its impurities, thus proved the stability indicating power of the method. The stress samples were assayed against a reference standard and the mass balance was found to be close to 99.1%. So this method was also suitable for Assay determination of Esomeprazole magnesium in pharmaceutical dosage forms. The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of detection, limit of quantification, accuracy, precision and robustness. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Safety of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole

The Article related to liquid chromatog impurity esomeprazole magnesium tablet stress, Pharmaceuticals: Pharmaceutics and other aspects.Safety of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On September 30, 2007, El Alaoui, Abdessamad; Schmidt, Frederic; Amessou, Mohamed; Sarr, Marianne; Decaudin, Didier; Florent, Jean-Claude; Johannes, Ludger published an article.Recommanded Product: 901770-40-9 The title of the article was Shiga toxin-mediated retrograde delivery of a topoisomerase I inhibitor prodrug. And the article contained the following:

A retrograde strategy: An innovative cancer-cell delivery concept exploits the naturally evolved characteristics of the Shiga toxin B-subunit (STxB) for the intracellular activation of a newly synthesized prodrug at the level of the biosynthetic/secretory pathway. Retrograde prodrug targeting allows its slow release, which should sustain the presence of the active principle in dividing tumor cells. The experimental process involved the reaction of N-(4-Hydroxyphenyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide(cas: 901770-40-9).Recommanded Product: 901770-40-9

The Article related to shiga toxin retrograde delivery topoisomerase i inhibitor prodrug, Pharmaceuticals: Pharmaceutics and other aspects.Recommanded Product: 901770-40-9

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem