Month: November 2022

Berzas Nevado, Juan Jose; Castaneda Penalvo, Gregorio; Rodriguez Dorado, Rosa M.; Rodriguez Robledo, Virginia published an article in 2013, the title of the article was Study of controlled degradation processes and electrophoretic behaviour of omeprazole and its main degradation products using diode-array and ESI-IT-MS detection.Safety of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole And the article contains the following content:

This paper provides chem. information of controlled degradation processes on omeprazole, and its main degradation products in aqueous solutions, using CE as the anal. technique. Both diode-array and ESI-IT-MS were employed as detectors to generate anal. data of the drugs studied. This information could be used to determine potential impurities for pharmaceuticals of omeprazole because it may influence not only the therapeutic efficacy but also the safety of active pharmaceutical ingredients. For this purpose, a new capillary zone electrophoresis method using diode-array detection for the simultaneous determination of omeprazole and its main degradation products was developed. Some different less aggressive degradation processes were studied, the fastest of which was found to be that of omeprazole in aqueous solutions exposed to UV light (254 nm). Using a background electrolyte (10 mM phosphate buffer, pH 12) the analytes were determined in < 6 min. Electrophoretic behavior using an optical detector revealed the presence of 7 products under the different exptl. conditions used. More anal. chem. information was obtained on omeprazole degradation products using direct infusion electrospray ionization-mass spectrometry operating in neg. ion mode [M - H]-, and even some chem. structures of omeprazole degradation products were proposed. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Safety of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole

The Article related to omeprazole degradation determination capillary zone electrophoresis impurity, Pharmaceutical Analysis: Synthetic Organic Compounds and other aspects.Safety of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 31, 2022, Shi, Hua; Li, Shuang; Su, Xi published an article.HPLC of Formula: 443-72-1 The title of the article was Plant6mA: A predictor for predicting N6-methyladenine sites with lightweight structure in plant genomes. And the article contained the following:

N6-methyladenine (6mA) in DNA, a type of DNA methylation in epigenetic modification, has attracted extensive attention in recent years. In order to improve our understanding of 6mA biol. activities and mechanisms in plant genomes, we need to be able to accurately identify 6mA sites. Because traditional wet-lab experiments frequently necessitate a large amount of manpower and time, a plethora of computational methods, particularly machine learning, have emerged to achieve fast and accurate 6mA site prediction. Traditional machine learning methods, on the other hand, rely heavily on manual features and integrated learning to improve performance, resulting in a reliance on prior knowledge and a large model scale. Furthermore, many models are only trained and tested for one species, with no comparison of model generalization performance, resulting in models with limited practical usability. In order to increase the generalization capability of the model, we propose a lightweight structure predictor Plant6mA based on Transformer encoder. Based on results on independent test sets, our proposed Plant6mA has better generalization performance than the most advanced methods in predicting 6mA location in plant genomes. Plant6mAs MultiHead Attention mechanism effectively enhances its expressive ability by capturing potential biol. information from multiple scales of the input sequence. Furthermore, we used a dimensionality reduction tool to visualize Plant6mAs training process and visually demonstrate the effectiveness of our model. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1

The Article related to plant6ma plant genome n6 methyladenine lightweight structure, bioinformatics, dna methylation, deep learning, Plant Biochemistry: Classical Genetics and Phylogeny and other aspects.HPLC of Formula: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Yuan, De-Hui; Xing, Jian-Feng; Luan, Mei-Wei; Ji, Kai-Kai; Guo, Jun; Xie, Shang-Qian; Zhang, Yuan-Ming published an article in 2020, the title of the article was DNA N6-methyladenine modification in wild and cultivated soybeans reveals different patterns in nucleus and cytoplasm.Computed Properties of 443-72-1 And the article contains the following content:

DNA 6mA modification, an important newly discovered epigenetic mark, plays a crucial role in organisms and has been attracting more and more attention in recent years. The soybean is economically the most important bean in the world, providing vegetable protein for millions of people. However, the distribution pattern and function of 6mA in soybean are still unknown. In this study, we decoded 6mA modification to single-nucleotide resolution in wild and cultivated soybeans, and compared the 6mA differences between cytoplasmic and nuclear genomes and between wild and cultivated soybeans. The motif of 6mA in the nuclear genome was conserved across the two kinds of soybeans, and ANHGA was the most dominant motif in wild and cultivated soybeans. Genes with 6mA modification in the nucleus had higher expression than those without modification. Interestingly, 6mA distribution patterns in cytoplasm for each soybean were significantly different from those in nucleus, which was reported for the first time in soybean. Our research provides a new insight in the deep anal. of cytoplasmic genomic DNA modification in plants. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Computed Properties of 443-72-1

The Article related to glycine genomic dna n6methyladenine nucleus cytoplasm, cultivated soybean, cytoplasm, methylation, nucleus, wild soybean, Plant Biochemistry: Classical Genetics and Phylogeny and other aspects.Computed Properties of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2013, Manranjan, Vayeda Chintan; Yadav, Devendra Singh; Jogia, Hitesh Amrutlal; Chauhan, Praful Lalitkumar published an article.Formula: C17H19N3O2S The title of the article was Design of experiment (DOE) utilization to develop a simple and robust reversed-phase HPLC technique for related substances’ estimation of omeprazole formulations. And the article contained the following:

A simple, fast, and sensitive reversed-phase HPLC method with UV detection was developed for the quantitation of omeprazole and its eleven related compounds (impurities) in pharmaceutical formulation using the Thermo Accucore C-18 (50 mm × 4.6 mm, 2.6 μm) column. The separation among all the compounds was achieved with a flow rate of 0.8 mL min-1 employing a gradient program of mobile phase A [0.08 M glycine buffer pH 9.0: acetonitrile; 95:05 (volume/volume)] and mobile phase B [acetonitrile: MeOH; 65:35 (volume/volume)]. The chromatog. detection was carried out at a wavelength of 305 nm. The method was validated for specificity, linearity, and recovery. The huskiness of the method was determined prior to validation using the Design of Experiments (DOE). The ANOVA anal. of DOE with a 95% confidence interval (CI) confirmed the buffer pH of mobile phase A and column temperature as significant Critical Method Parameters (CMPs). The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Formula: C17H19N3O2S

The Article related to omeprazole impurity determination rp hplc, anova, chromatography, compatible, doe, hplc/uplc, method development, related substances, Pharmaceutical Analysis: Synthetic Organic Compounds and other aspects.Formula: C17H19N3O2S

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On June 30, 2013, Seshadri, Raja Kumar; Raghavaraju, Thummala Veera; Chakravarthy, Ivon Elisha published an article.Category: imidazoles-derivatives The title of the article was A single gradient stability-indicating reversed-phase LC method for the estimation of impurities in omeprazole and domperidone capsules. And the article contained the following:

A gradient reversed-phase liquid chromatog. (RP-LC) method was developed for the quant. estimation of impurities in the pharmaceutical dosage form of Omeprazole and Domperidone capsules. The developed method is a stability-indicating test method for the estimation of impurities generated during the formulation and storage of Omeprazole and Domperidone capsules. The chromatog. separation was achieved on a column packed with octadecyl silane, having a column length of 250 mm and diameter of 4.6 mm with a particle size of 5 μm, and by following a gradient program using a combination of a monobasic potassium phosphate buffer (0.05M) and acetonitrile. Since the spectral properties were similar, both compounds’ individual impurities were estimated at 285 nm. Forced degradation studies were performed on Omeprazole pellets (enteric coated) and Domperidone pellets (SR coated) encapsulated in size “1” hard gelatin capsules. Omeprazole and Domperidone were degraded using acid hydrolysis (0.1 N hydrochloric acid), base (0.1 N sodium hydroxide), oxidation (50% hydrogen peroxide), heat (105 °C), and UV light (254 nm). The established method was validated and found to be linear, accurate, precise, specific, robust, and rugged. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Category: imidazoles-derivatives

The Article related to omeprazole domperidone impurity determination rp hplc capsule degradation, domperidone, forced degradation, method validation, omeprazole, rp-lc, Pharmaceutical Analysis: Synthetic Organic Compounds and other aspects.Category: imidazoles-derivatives

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 15, 2020, Rios-Gomez, Julia; Garcia-Valverde, Maria Teresa; Lopez-Lorente, Angela Inmaculada; Toledo-Neira, Carla; Lucena, Rafael; Cardenas, Soledad published an article.Safety of N-(3-Aminopropyl)-imidazole The title of the article was Polymeric ionic liquid immobilized onto paper as sorptive phase in microextraction. And the article contained the following:

A new planar sorptive phase based on the simple immobilization of polymeric ionic liquids on paper is proposed. The sorptive phase can develop hydrophobic or mixed-mode (combining hydrophobic and ion exchange) interactions with the target analytes. The polymer is prepared by the Radziszewski reaction, which takes place in aqueous media, and it has been thoroughly characterized by different techniques including IR spectroscopy, matrix-assisted laser desorption/ionization coupled to high-resolution mass spectrometry and proton NMR. Three different strategies aimed to immobilize the polymeric ionic liquid on paper have been evaluated. Among them, simple thermal curing at 120°C was selected. The as-prepared paper has been evaluated for the extraction of several non-steroidal anti-inflammatory drugs from urine, the analytes being finally determined by liquid chromatog. with tandem mass spectrometry. The method detection limits were 3.8, 7.2, 6.8, 9.4, 15.7, and 5.1μg/L for indomethacin, diclofenac, tolmetin, ketoprofen, naproxen, and ibuprofen, resp. Calibration models were linear (R2 > 0.9949) up to 1000μg/L. The intra-day precision, expressed as relative standard deviation and calculated at three different concentrations levels (limit of quantification, 250μg/L, and 1000μg/L), varied between 1.1 and 13%. The accuracy, calculated as relative recovery, was in the range from 72 to 95%, thus being considered appropriate. The easiness of polymeric ionic liquid paper synthesis and the multi-sample extraction protocol designed allows the processing of a high number of samples at the same time. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Safety of N-(3-Aminopropyl)-imidazole

The Article related to polymeric ionic liquid paper microextraction drug, bioanalysis, microextraction, paper substrate, polymeric ionic liquids, radziszewski reaction, Pharmaceutical Analysis: Synthetic Organic Compounds and other aspects.Safety of N-(3-Aminopropyl)-imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 25, 2016, Jadhav, Sushant Bhimrao; Kumar, C. Kiran; Bandichhor, Rakeshwar; Bhosale, P. N. published an article.Electric Literature of 73590-85-9 The title of the article was Development of RP UPLC-TOF/MS, stability indicating method for omeprazole and its related substances by applying two level factorial design; and identification and synthesis of non-pharmacopoeial impurities. And the article contained the following:

A new UPLC-TOF/MS compatible, reverse phase-stability indicating method was developed for determination of Omeprazole (OMP) and its related substances in pharmaceutical dosage forms by implementing Design of Experiment (DoE) i.e. two level full factorial Design (23 + 3 center points = 11 experiments) to understand the Critical Method Parameters (CMP) and its relation with Critical Method Attribute (CMA); to ensure robustness of the method. The separation of eleven specified impurities including conversion product of OMP related compound F (13) and G (14) i.e. Impurity-I (1), OMP related compound-I (11) and OMP 4-chloro analog (12) was achieved in a single method on Acquity BEH shield RP18 100 × 2.1 mm, 1.7 μm column, with inlet filter (0.2 μm) using gradient elution and detector wavelength at 305 nm and validated in accordance with ICH guidelines and found to be accurate, precise, reproducible, robust and specific. The drug was found to degrade extensively in heat, humidity and acidic conditions and forms unknown degradation products during stability studies. The same method was used for LC-MS anal. to identify m/z and fragmentation of maximum unknown impurities (Non-Pharmacopoeial) i.e. Impurity-I (1), Impurity-III (3), Impurity-V (5) and Impurity-VIII (9) formed during stability studies. Based on the results, degradation pathway for the drug has been proposed and synthesis of identified impurities i.e. impurities (Impurity-I (1), Impurity-III (3), Impurity-V (5) and Impurity-VIII (9)) are discussed in detail to ensure in-depth understanding of OMP and its related impurities and optimum performance during lifetime of the product. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Electric Literature of 73590-85-9

The Article related to uplc tof ms omeprazole related substances factorial design impurity, design of experiments (doe), development, full factorial design, omeprazole, uplc-tof/ms, validation, Pharmaceutical Analysis: Synthetic Organic Compounds and other aspects.Electric Literature of 73590-85-9

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On May 5, 2022, Larivera, Simone; Meister, Gunter published an article.Reference of N-Methyl-7H-purin-6-amine The title of the article was Domain confusion 2: m6 A-independent role of YTHDC2. And the article contained the following:

YTH proteins utilize YTH domains to interact with N6-methyladenines (m6A); however, Li et al. (2022) show that YTHDC2 binds U-rich motifs instead and functions independently of m6A through its unusual DExD helicase domain during spermatogenesis in mice and fish. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine

The Article related to methyladenine domain confusion, General Biochemistry: Proteins and Their Constituents and other aspects.Reference of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Yu, Dan; Horton, John R.; Yang, Jie; Hajian, Taraneh; Vedadi, Masoud; Sagum, Cari A.; Bedford, Mark T.; Blumenthal, Robert M.; Zhang, Xing; Cheng, Xiaodong published an article in 2021, the title of the article was Human MettL3-MettL14 RNA adenine methyltransferase complex is active on double-stranded DNA containing lesions.Application In Synthesis of N-Methyl-7H-purin-6-amine And the article contains the following content:

MettL3-MettL14 methyltransferase complex has been studied widely for its role in RNA adenine methylation. This complex is also recruited to UV- and X-ray exposed DNA damaged sites, and its methyltransfer activity is required for subsequent DNA repair, though in theory this could result from RNA methylation of short transcripts made at the site of damage. We report here that MettL3-MettL14 is active in vitro on double-stranded DNA containing a cyclopyrimidine dimer – a major lesion of UV radiation-induced products – or an abasic site or mismatches. Furthermore, N6-methyladenine (N6mA) decreases misincorporation of 8-oxo-guanine (8-oxoG) opposite to N6mA by repair DNA polymerases. When 8-oxoG is nevertheless incorporated opposite N6mA, the methylation inhibits N6mA excision from the template (correct) strand by the adenine DNA glycosylase (MYH), implying that the methylation decreases inappropriate misrepair. Finally, we observed that the N6mA reader domain of YTHDC1, which is also recruited to sites of DNA damage, binds N6mA that is located across from a single-base gap between two canonical DNA helixes. This YTHDC1 complex with a gapped duplex is structurally similar to DNA complexes with FEN1 and GEN1 – two members of the nuclease family that act in nucleotide excision repair, mismatch repair and homologous recombination, and which incise distinct non-B DNA structures. Together, the parts of our study provide a plausible mechanism for N6mA writer and reader proteins acting directly on lesion-containing DNA, and suggest in vivo experiments to test the mechanisms involving methylation of adenine. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to mettl3 mettl14 double stranded dna lesion, Biochemical Genetics: Gene Structure and Organization and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Rodriguez, Fernando; Yushenova, Irina A.; DiCorpo, Daniel; Arkhipova, Irina R. published an article.SDS of cas: 443-72-1 The title of the article was Bacterial N4-methylcytosine as an epigenetic mark in eukaryotic DNA. And the article contained the following:

DNA modifications are used to regulate gene expression and defend against invading genetic elements. In eukaryotes, modifications predominantly involve C5-methylcytosine (5mC) and occasionally N6-methyladenine (6mA), while bacteria frequently use N4-methylcytosine (4mC) in addition to 5mC and 6mA. Here we report that 4mC can serve as an epigenetic mark in eukaryotes. Bdelloid rotifers, tiny freshwater invertebrates with transposon-poor genomes rich in foreign genes, lack canonical eukaryotic C5-methyltransferases for 5mC addition, but encode an amino-methyltransferase, N4CMT, captured from bacteria >60 Mya. N4CMT deposits 4mC at active transposons and certain tandem repeats, and fusion to a chromodomain shapes its “histone-read-DNA-write” architecture recognizing silent chromatin marks. Furthermore, amplification of SETDB1 H3K9me3 histone methyltransferases yields variants preferentially binding 4mC-DNA, suggesting “DNA-read-histone-write” partnership to maintain chromatin-based silencing. Our results show how non-native DNA Me groups can reshape epigenetic systems to silence transposons and demonstrate the potential of horizontal gene transfer to drive regulatory innovation in eukaryotes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to bdelloid epigenetics n4 methylcytosine dna, General Biochemistry: Proteins and Their Constituents and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem