Month: November 2022

On October 1, 2007, Srinath, Thiruneelakantan; Bharti, Sanjay Kumar; Varshney, Umesh published an article.Synthetic Route of 55662-66-3 The title of the article was Substrate specificities and functional characterization of a thermo-tolerant uracil DNA glycosylase (UdgB) from Mycobacterium tuberculosis. And the article contained the following:

Uracil DNA glycosylases (UDGs) excise uracil from DNA and initiate the base (uracil) excision repair pathway. Ung, a highly conserved protein, is the only UDG characterized so far in mycobacteria. Here, we show that Rv1259 from Mycobacterium tuberculosis codes for a double-stranded DNA (dsDNA) specific UDG (MtuUdgB). MtuUdgB is thermo-tolerant, contains Fe-S cluster and, in addition to uracil, it excises ethenocytosine and hypoxanthine from dsDNA. MtuUdgB is product inhibited by AP-site containing dsDNA but not by uracil. While MtuUdgB excises uracil present as a single-nucleotide bulge in dsDNA, it is insensitive to inhibition by dsDNA containing AP-site in the bulge. Interestingly, in the presence of cellular factors, the uracil excision activity of MtuUdgB is enhanced, and when introduced into E. coli (ung-), it rescues its mutator phenotype and prevents C to T mutations in DNA. Novel features of the mechanism of action of MtuUdgB and the physiol. significance of the family 5 UDG in mycobacteria have been discussed. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Synthetic Route of 55662-66-3

The Article related to thermotolerant uracil dna glycosylase udgb mycobacterium, Enzymes: Separation-Purification-General Characterization and other aspects.Synthetic Route of 55662-66-3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Fu, Dragony; Samson, Leona D. published an article in 2012, the title of the article was Direct repair of 3,N 4-ethenocytosine by the human ALKBH2 dioxygenase is blocked by the AAG/MPG glycosylase.Recommanded Product: 55662-66-3 And the article contains the following content:

Exocyclic ethenobases are highly mutagenic DNA lesions strongly implicated in inflammation and vinyl chloride-induced carcinogenesis. While the alkyladenine DNA glycosylase, AAG (or MPG), binds the etheno lesions 1,N 6-ethenoadenine (εA) and 3,N 4-ethenocytosine (εC) with high affinity, only εA can be excised to initiate base excision repair. Here, we discover that the human AlkB homolog 2 (ALKBH2) dioxygenase enzyme catalyzes direct reversal of εC lesions in both double- and single-stranded DNA with comparable efficiency to canonical ALKBH2 substrates. Notably, we find that in vitro, the non-enzymic binding of AAG to εC specifically blocks ALKBH2-catalyzed repair of εC but not that of methylated ALKBH2 substrates. These results identify human ALKBH2 as a repair enzyme for mutagenic εC lesions and highlight potential consequences for substrate-binding overlap between the base excision and direct reversal DNA repair pathways. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Recommanded Product: 55662-66-3

The Article related to alkbh2 dioxygenase human dna repair ethenocytosine inhibition aag glycosylase, Enzymes: Separation-Purification-General Characterization and other aspects.Recommanded Product: 55662-66-3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 22, 2000, Sung, Jung-Suk; Mosbaugh, Dale W. published an article.SDS of cas: 55662-66-3 The title of the article was Escherichia coli Double-Strand Uracil-DNA Glycosylase: Involvement in Uracil-Mediated DNA Base Excision Repair and Stimulation of Activity by Endonuclease IV. And the article contained the following:

Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified to apparent homogeneity as both a native and recombinant protein. The mol. weight of recombinant Dug was 18,670, as determined by matrix-assisted laser desorption-ionization mass spectrometry. Dug was active on duplex oligonucleotides (34-mers) that contained site-specific U·G, U·A, ethenoC·G, and ethenoC·A targets; however, activity was not detected on DNA containing a T·G mispair or single-stranded DNA containing either a site-specific uracil or ethenoC residue. One of the distinctive characteristics of Dug was that the purified enzyme excised a near stoichiometric amount of uracil from U·G-containing oligonucleotide substrate. Electrophoretic mobility shift assays revealed that the lack of turnover was the result of strong binding by Dug to the reaction product apyrimidinic-site (AP) DNA. Addition of E. coli endonuclease IV stimulated Dug activity by enhancing the rate and extent of uracil excision by promoting dissociation of Dug from the AP·G-containing 34-mer. Catalytically active endonuclease IV was apparently required to mediate Dug turnover, since the addition of 5 mM EDTA mitigated the effect. Further support for this interpretation came from the observations that Dug preferentially bound 34-mer containing an AP·G target, while binding was not observed on a substrate incised 5′ to the AP-site. We also investigated whether Dug could initiate a uracil-mediated base excision repair pathway in E. coli NR8052 cell extracts using M13mp2op14 DNA (form I) containing a site-specific U·G mispair. Anal. of reaction products revealed a time dependent appearance of repaired form I DNA; addition of purified Dug to the cell extract stimulated the rate of repair. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).SDS of cas: 55662-66-3

The Article related to escherichia dsdna uracil dna glycosylase, endonuclease iv dsdna uracil dna glycosylase stimulation, Enzymes: Separation-Purification-General Characterization and other aspects.SDS of cas: 55662-66-3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2022, Kohlgruber, Tsuyoshi A.; Perry, Samuel N.; Sigmon, Ginger E.; Oliver, Allen G.; Burns, Peter C. published an article.Application of 5036-48-6 The title of the article was Hydrogen bond network and bond valence analysis on uranyl sulfate compounds with organic-based interstitial cations. And the article contained the following:

Seven new uranyl sulfate compounds with organic charge-balancing cations were synthesized and structurally characterized. The structural unit topologies of the two chains and five sheets were previously reported in uranyl sulfate crystal chem., although they were synthesized using different organic mols. With the inclusion of six of these structures to the 48 previously published uranyl sulfate compounds, a total of 54 known uranyl sulfate compounds with organic charge-balancing cations were compiled and analyzed. A graphical approach was used to compare the structural unit topologies, and a bond valence approach was used to quantify the H bond networks that exist between the interstitial cationic and solvent species to the uranyl sulfate anionic structural units. This anal. helped elucidate which O atoms in the structural unit receive H bonds and how the organic cations stabilize the overall crystal structures in this subclass of U(VI) materials. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Application of 5036-48-6

The Article related to uranyl sulfate anion preparation hydrogen bond network, crystal structure uranyl sulfate interstitial organic cation, Inorganic Chemicals and Reactions: Coordination Compounds and other aspects.Application of 5036-48-6

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On February 28, 2021, Cao, Yue; Zhou, Xiaoying; Luan, Lindong; Zeng, Hongmei; Zou, Guohong; Lin, Zhien published an article.Quality Control of N-(3-Aminopropyl)-imidazole The title of the article was Organically templated metal phosphate-oxalates: Solvent-free synthesis, crystal structure, and proton conduction. And the article contained the following:

Two new metal phosphate-oxalates, namely, H2api·Mn2(H2PO4)2(C2O4)2 (1) and H2mpip·Sc(H2PO4)2(C2O4)·0.5C2O4·1.5H2O (2), were prepared under solvent-free conditions, where api = 1-(3-aminopropyl)imidazole and mpip = 1-methylpiperazine. Compound 1 has a honeycomb-like structure with 12-ring windows. Compound 2 has a one-dimensional structure with scandium phosphate ladders decorated with oxalate ligands. The proton-conducting behaviors of this compound under 95% relative humidity were investigated. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Quality Control of N-(3-Aminopropyl)-imidazole

The Article related to manganese scandium phosphate oxalate preparation crystal mol structure proton, proton conduction metal manganese scandium phosphate oxalate, Inorganic Chemicals and Reactions: Coordination Compounds and other aspects.Quality Control of N-(3-Aminopropyl)-imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On May 12, 2021, Wang, Qing; Tan, Kaiyue; Wang, Hong; Shang, Jinhua; Wan, Yeqing; Liu, Xiaoqing; Weng, Xiaocheng; Wang, Fuan published an article.Safety of N-Methyl-7H-purin-6-amine The title of the article was Orthogonal demethylase-activated deoxyribozyme for intracellular imaging and gene regulation. And the article contained the following:

The epigenetic modification of nucleic acids represents a versatile approach for achieving high-efficient control over gene expression and transcription and could dramatically expand their biosensing and therapeutic applications. Demethylase-involved removal of N6-methyladenine (m6A) represents one of the vital epigenetic reprogramming events, yet its direct intracellular evaluation and as-guided gene regulation are extremely rare. The endonuclease-mimicking deoxyribozyme (DNAzyme) is a catalytically active DNA that enables the site-specific cleavage of the RNA substrate, and several strategies have imparted the magnificent responsiveness to DNAzyme by using chem. and light stimuli. However, the epigenetic regulation of DNAzyme has remained largely unexplored, leaving a significant gap in responsive DNA nanotechnol. Herein, we reported an epigenetically responsive DNAzyme system through the in vitro selection of an exquisite m6A-caged DNAzyme that could be specifically activated by FTO (fat mass and obesity-associated protein) demethylation for precise intracellular imaging-directed gene regulation. Based on a systematic investigation, the active DNAzyme configuration was potently disrupted by the site-specific incorporation of m6A modification and subsequently restored into the intact DNAzyme structure via the tunable FTO-specific removal of m6A-caging groups under a variety of conditions. This orthogonal demethylase-activated DNAzyme amplifier enables the robust and accurate monitoring of FTO and its inhibitors in live cells. Moreover, the simple demethylase-activated DNAzyme facilitates the assembly of an intelligent self-adaptive gene regulation platform for knocking down demethylase with the ultimate apoptosis of tumor cells. As a straightforward and scarless m6A removal strategy, the demethylase-activated DNAzyme system offers a versatile toolbox for programmable gene regulation in synthetic biol. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to dnazyme rnase regulation n6 methyladenine cage demethylase fto protein, human cell demethylase activated dnazyme programmable gene regulation, Enzymes: Separation-Purification-General Characterization and other aspects.Safety of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On September 24, 2020, Kilic, Ahmet; Beyazsakal, Levent; Findik, Bahar Tuba; Incebay, Hilal published an article.Application In Synthesis of N-(3-Aminopropyl)-imidazole The title of the article was Synthesis and electrochemical investigation of chiral amine bis(phenolate)-boron complexes: In vitro antibacterial activity screening of boron compounds. And the article contained the following:

A new class of low-cost, easily-synthesizable and modifiable chiral amine bis(phenolate) ligand (L), its chiral boron complex (LB), and five different salen groups (1-(3-Aminopropyl) imidazole (LB1), N,N-Diethyl-p-phenylenediamine (LB2), 2-Picolylamine (LB3), 4′-Aminoacetophenone (LB4), and 4-Amino-2,2,6,6-tetramethyl piperidine (LB5)) containing chiral boron complexes were synthesized. These newly synthesized chiral compounds were fully characterized by 1H and 13C NMR, FTIR, UV-visible, and LC-MS/MS spectroscopy, m.p., elemental anal., and cyclic voltammetry techniques. The in vitro antibacterial activity of the synthesized different chiral boron complexes was tested against four pathogenic bacteria strains using the resazurin-based broth microdilution method, and the MIC values of each boron complex were determined Based on the overall results, the N, N-Diethyl-p-phenylenediamine group containing chiral boron complex (LB2) showed the highest activity against all bacterial strains, with the lowest MIC value of 4μg/mL which is nearly in the range of values for com. antibacterial drugs. The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Application In Synthesis of N-(3-Aminopropyl)-imidazole

The Article related to preparation boron chiral amine phenolate complex, cyclic voltammetry boron chiral amine phenolate complex, antimicrobial activity boron chiral amine phenolate complex, Inorganic Chemicals and Reactions: Coordination Compounds and other aspects.Application In Synthesis of N-(3-Aminopropyl)-imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Talhaoui, Ibtissam; Couve, Sophie; Ishchenko, Alexander A.; Kunz, Christophe; Schaer, Primo; Saparbaev, Murat published an article in 2013, the title of the article was 7,8-dihydro-8-oxoadenine, a highly mutagenic adduct, is repaired by Escherichia coli and human mismatch-specific uracil/thymine-DNA glycosylases.Computed Properties of 55662-66-3 And the article contains the following content:

Hydroxyl radicals predominantly react with the C8 of purines in DNA forming 7,8-dihydro-8-oxoguanine (8oxoG) and 7,8-dihydro-8-oxoadenine (8oxoA) adducts, which are highly mutagenic in mammalian cells. The majority of oxidized DNA bases are removed by DNA glycosylases in the base excision repair pathway. Here, the authors report for the 1st time that human thymine-DNA glycosylase (hTDG) and Escherichia coli mismatch-specific uracil-DNA glycosylase (MUG) can remove 8oxoA from 8oxoA·T, 8oxoA·G, and 8oxoA·C pairs. Comparison of the kinetic parameters of the reaction indicated that full-length hTDG excised 8oxoA, 3,N4-ethenocytosine (εC) and T with similar efficiency (kmax = 0.35, 0.36, and 0.16 min-1, resp.) and was more proficient as compared with its bacterial homolog MUG. The N-terminal domain of the hTDG protein was essential for 8oxoA-DNA glycosylase activity, but not for εC repair. Interestingly, the TDG status had little or no effect on the proliferation rate of mouse embryonic fibroblasts after exposure to γ-irradiation Nevertheless, using whole cell-free extracts from DNA glycosylase-deficient murine embryonic fibroblasts and E. coli, the authors demonstrated that the excision of 8oxoA from 8oxoA·T and 8oxoA·G had an absolute requirement for TDG and MUG, resp. The data established that MUG and TDG can counteract the genotoxic effects of 8oxoA residues in vivo. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Computed Properties of 55662-66-3

The Article related to dna repair oxoadenine removal uracil thymine glycosylase, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.Computed Properties of 55662-66-3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Aliakbar Tehrani, Zahra; Torabifard, Hedieh; Fattahi, Alireza published an article in 2012, the title of the article was Thermochemical properties of some vinyl chloride-induced DNA lesions: detailed view from NBO & AIM analysis.Name: Imidazo[1,2-c]pyrimidin-5(6H)-one And the article contains the following content:

Etheno-damaged DNA adducts such as 3,N4-ethenocytosine, N2,3-ethenoguanine, and 1,N2-ethenoguanine are associated with carcinogenesis and cell death. These inevitable damages are counteracted by glycosylase enzymes, which cleave damaged nucleobases from DNA. Escherichia coli alkyl purine DNA glycosylase is the enzyme responsible for excising damaged etheno adducts from DNA in humans. In an effort to understand the intrinsic properties of these mols., we examined gas-phase acidity values and proton affinities (PA) of multiple sites of these mols. as well as equilibrium tautomerization and base pairing properties by quantum mech. calculations We also used calculations to compare the acidic and basic properties of these etheno adduct with those of the normal bases-cytosine and guanine nucleobases. We hypothesize that alkyl DNA glycosylase may cleave certain damaged nucleobases as anions and that the active site may take advantage of a nonpolar environment to favor deprotonated cytosine or guanine as a leaving group vs. damaged nucleobases. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Name: Imidazo[1,2-c]pyrimidin-5(6H)-one

The Article related to dna damage thermochem property ethenocytosine ethenoguanine, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.Name: Imidazo[1,2-c]pyrimidin-5(6H)-one

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On September 13, 2012, Srinivasadesikan, Venkatesan; Sahu, Prabhat K.; Lee, Shyi-Long published an article.Synthetic Route of 55662-66-3 The title of the article was Quantum Mechanical Calculations for the Misincorporation of Nucleotides Opposite Mutagenic 3,N4-Ethenocytosine. And the article contained the following:

The ubiquitous nature and persistence of exocyclic DNA adducts suggest their involvement as initiators of carcinogenesis. We have investigated the misincorporation properties of the exocyclic DNA adduct, 3,N4-ethenocytosine (εC), using DFT and DFT-D methods. Computational investigations have been carried out by using the B3LYP, M062X, and wB97XD methods with the 6-31+G* basis set to determine the hydrogen bonding strengths, binding energy, and phys. parameters. The single point energy calculations have been carried out at MP2/6-311++G** on corresponding optimized geometries. The energies were compared among the 3,N4-ethenocytosine adduct with DNA bases to find the most stable conformer. The solvent phase calculations have also been carried out using the CPCM model. The computed reaction enthalpy values provide computational insights to the earlier exptl. observation in in vitro, E.coli, and mammalian cells of a high level of substitution mutation in which C → A transversion results from εC-T pairing [εC-T3 and εC-T4] in the adduct containing DNA sequence. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Synthetic Route of 55662-66-3

The Article related to ethenocytosine base pairing mismatch nucleotide misincorporation dna, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.Synthetic Route of 55662-66-3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem