Month: November 2022

On December 31, 2020, Wang, Hao-Tian; Xiao, Fu-Hui; Li, Gong-Hua; Kong, Qing-Peng published an article.Reference of N-Methyl-7H-purin-6-amine The title of the article was Identification of DNA N6-methyladenine sites by integration of sequence features. And the article contained the following:

Abstract: Background: An increasing number of nucleic acid modifications have been profiled with the development of sequencing technologies. DNA N6-methyladenine (6mA), which is a prevalent epigenetic modification, plays important roles in a series of biol. processes. So far, identification of DNA 6mA relies primarily on time-consuming and expensive exptl. approaches. However, in silico methods can be implemented to conduct preliminary screening to save exptl. resources and time, especially given the rapid accumulation of sequencing data. Results: In this study, we constructed a 6mA predictor, p6mA, from a series of sequence-based features, including physicochem. properties, position-specific triple-nucleotide propensity (PSTNP), and electron-ion interaction pseudopotential (EIIP). We performed maximum relevance maximum distance (MRMD) anal. to select key features and used the Extreme Gradient Boosting (XGBoost) algorithm to build our predictor. Results demonstrated that p6mA outperformed other existing predictors using different datasets. Conclusions: p6mA can predict the methylation status of DNA adenines, using only sequence files. It may be used as a tool to help the study of 6mA distribution pattern. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine

The Article related to dna nitrogen methyladenine algorimthm, dna n6-methyladenine, machine learning, xgboost, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Reference of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2021, Chaturvedi, Anurag; Cruz Corella, Joaquim; Robbins, Chanz; Loha, Anita; Menin, Laure; Gasilova, Natalia; Masclaux, Frederic G.; Lee, Soon-Jae; Sanders, Ian R. published an article.Formula: C6H7N5 The title of the article was The methylome of the model arbuscular mycorrhizal fungus, Rhizophagus irregularis, shares characteristics with early diverging fungi and Dikarya. And the article contained the following:

Early-diverging fungi (EDF) are distinct from Dikarya and other eukaryotes, exhibiting high N6-methyldeoxyadenine (6mA) contents, rather than 5-methylcytosine (5mC). As plants transitioned to land the EDF sub-phylum, arbuscular mycorrhizal fungi (AMF; Glomeromycotina) evolved a symbiotic lifestyle with 80% of plant species worldwide. Here we show that these fungi exhibit 5mC and 6mA methylation characteristics that jointly set them apart from other fungi. The model AMF, R. irregularis, evolved very high levels of 5mC and greatly reduced levels of 6mA. However, unlike the Dikarya, 6mA in AMF occurs at sym. ApT motifs in genes and is associated with their transcription. 6MA is heterogeneously distributed among nuclei in these coenocytic fungi suggesting functional differences among nuclei. While far fewer genes are regulated by 6mA in the AMF genome than in EDF, most strikingly, 6mA methylation has been specifically retained in genes implicated in components of phosphate regulation; the quintessential hallmark defining this globally important symbiosis. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to rhizophagus irregularis arbuscular mycorrhizal fungus characteristic methyldeoxyadenine methylcytosine, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Song, Weiguo; Dong, Liangjun; Zhou, Yuhan; Fu, Yongqiang; Xu, Wenfang published an article in 2015, the title of the article was Catalytic Asymmetric Synthesis of Esomeprazole by a Titanium Complex with a Hexa-aza-triphenolic Macrocycle Ligand.Quality Control of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole And the article contains the following content:

An efficient synthesis of esomeprazole I via catalytic asym. oxidation of 1H-benzimidazolyl pyridinylmethyl sulfide by a titanium complex with a hexa-aza-triphenolic macrocycle ligand is described. Esomeprazole was prepared with 99.6% ee, which meets the high requirement of the European Pharmacopeia on enantiomeric purity. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Quality Control of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole

The Article related to esomeprazole enantioselective synthesis, benzimidazolyl pyridinylmethyl sulfide oxidation titanium complex hexaazatriphenolic macrocycle ligand, Heterocyclic Compounds (More Than One Hetero Atom): Pyrazoles and other aspects.Quality Control of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2021, Chen, Zheng; Liu, Yan; Wang, Hailin published an article.Application of 443-72-1 The title of the article was Conjoint expression and purification strategy for acquiring proteins with ultra-low DNA N6-methyladenine backgrounds in Escherichia coli. And the article contained the following:

DNA N6-methyladenine (6mA), a kind of DNA epigenetic modification, is widespread in eukaryotes and prokaryotes. An enzyme activity study coupled with 6mA detection using ultra-high-performance liquid chromatog.-quadruple mass spectrometry (UHPLC-MS/MS) is commonly applied to investigate 6mA potentially related enzymes in vitro. However, the protein expressed in a common Escherichia coli (E. coli) strain shows an extremely high 6mA background due to minute co-purified bacterial DNA, though it has been purified to remove DNA using multiple strategies. Furthermore, as occupied by DNA with abundant 6mA, the activity of 6mA-related proteins will be influenced seriously. Here, to address this issue, we for the first time construct a derivative of E. coli Rosetta (DE3) via the λRed knockout system specifically for the expression of 6mA-related enzymes. The gene dam encoding the 6mA methyltransferase (MTase) is knocked out in the newly constructed strain named LAMBS (low adenine methylation background strain). Contrasting with E. coli Rosetta (DE3), LAMBS shows an ultra-low 6mA background on the genomic DNA when analyzed by UHPLC-MS/MS. We also demonstrate an integral strategy of protein purification, coupled with the application of LAMBS. As a result, the purified protein expressed in LAMBS exhibits an ultra-low 6mA background comparing with the one expressed in E. coli Rosetta (DE3). Our integral strategy of protein expression and purification will benefit the in vitro investigation and application of 6mA-related proteins from eukaryotes, although these proteins are elusive until now. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to escherichia dna n6 methyladenine protein expression purification, dna n6-methyladenine, e. coli rosetta (de3), uhplc-ms/ms, low background, λred system, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Yang, Xiao; Ye, Xiucai; Li, Xuehong; Wei, Lesong published an article in 2021, the title of the article was iDNA-MT: identification DNA modification sites in multiple species by using multi-task learning based a neural network tool.Quality Control of N-Methyl-7H-purin-6-amine And the article contains the following content:

DNA N4-methylcytosine (4mC) and N6-methyladenine (6mA) are two important DNA modifications and play crucial roles in a variety of biol. processes. Accurate identification of the modifications is essential to better understand their biol. functions and mechanisms. However, existing methods to identify 4mA or 6mC sites are all single tasks, which demonstrates that they can identify only a certain modification in one species. Therefore, it is desirable to develop a novel computational method to identify the modification sites in multiple species simultaneously. In this study, we proposed a computational method, called iDNA-MT, to identify 4mC sites and 6mA sites in multiple species, resp. The proposed iDNA-MT mainly employed multi-task learning coupled with the bidirectional gated recurrent units (BGRU) to capture the sharing information among different species directly from DNA primary sequences. Exptl. comparative results on two benchmark datasets, containing different species resp., show that either for identifying 4mA or for 6mC site in multiple species, the proposed iDNA-MT outperforms other state-of-the- art single-task methods. The promising results have demonstrated that iDNA-MT has great potential to be a powerful and practically useful tool to accurately identify DNA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Quality Control of N-Methyl-7H-purin-6-amine

The Article related to dna modification site multi task learning neural network tool, dna modification, deep learning, feature representation, multi-task learning, neural network, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Quality Control of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2021, Joshi, Suraj; Ujaoney, Aman Kumar; Ghosh, Payel; Deobagkar, Deepti D.; Basu, Bhakti published an article.Name: N-Methyl-7H-purin-6-amine The title of the article was N6-methyladenine and epigenetic immunity of Deinococcus radiodurans. And the article contained the following:

DNA methylation is ubiquitously found in all three domains of life. This epigenetic modification on adenine or cytosine residues serves to regulate gene expression or to defend against invading DNA in bacteria. Here, we report the significance of N6-methyladenine (6mA) to epigenetic immunity in Deinococcus radiodurans. Putative protein encoded by DR_2267 ORF (Dam2DR) contributed 35% of genomic 6mA in D. radiodurans but did not influence gene expression or radiation resistance. Dam2DR was characterized to be a functional S-adenosyl methionine (SAM)-dependent N6-adenine DNA methyltransferase (MTase) but with no endonuclease activity. Adenine methylation from Dam2DR or Dam1DR (N6-adenine MTase encoded by DR_0643) improved DNA uptake during natural transformation. To the contrary, methylation from Escherichia coli N6-adenine MTase (DamEC that methylates adenine in GATC sequence) on donor plasmid drastically reduced DNA uptake in D. radiodurans, even in presence of Dam2DR or Dam1DR methylated adenines. With these results, we conclude that self-type N6-adenine methylation on donor DNA had a protective effect in absence of addnl. foreign methylation, a sep. methylation-dependent Restriction Modification (R-M) system effectively identifies and limits uptake of G6mATC sequence containing donor DNA. This is the first report demonstrating presence of epigenetic immunity in D. radiodurans. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to deinococcus n6 methyladenine epigenetic immunity, dna uptake, dr_2267, deinococcus radiodurans, n6-methyladenine, restriction modification, transformation efficiency, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Stensio, Karl E.; Wahlberg, Kerstin; Wahren, Robert published an article in 1973, the title of the article was Synthesis of brominated imidazoles.COA of Formula: C4H3BrN2O And the article contains the following content:

Bromination of imidazole in HOAc containing NaOAc gives 2,4,5-tribromoimidazole (I) in a good yield. 4(5)-(2-Chloroethyl)imidazole, imidazole-4(5)-carboxaldehyde, and 4(5)-hydroxymethylimidazole were similarly brominated. Debromination of I with Na2SO3 or BuLi gives 4(5)-bromoimidazole. A simple synthesis of 4(5)-(2-bromoethyl)imidazole is described. The experimental process involved the reaction of 5-Bromo-1H-imidazole-4-carbaldehyde(cas: 50743-01-6).COA of Formula: C4H3BrN2O

The Article related to bromination imidazole, Heterocyclic Compounds (More Than One Hetero Atom): Imidazoles and other aspects.COA of Formula: C4H3BrN2O

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On February 10, 2009, Wan, Huan; Fang, Feng; Duan, Mei-Li; Xu, Xu; Ji, Ya-Fei published an article.Reference of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole The title of the article was Synthesis of 6-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-1H-benzimidazole (omeprazole). And the article contained the following:

A method for the synthesis of the title compound [i.e., 6-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-1H-benzimidazole, omeprazole] is reported here. Omeprazole is a proton pump inhibitor. A one-pot method was performed for the oxidation and nitration of of 2,3,5-trimethylpyridine to provide 2,3,5-dimethylpyridine N-oxide. The chlorination of that intermediate gave 2-(chloromethyl)-3,5-dimethyl-4-nitropyridine 1-oxide. A reaction of this chloromethyl compound with 2-mercapto-5-methoxybenzimidazole in the presence of excess sodium methoxide simultaneously achieved a condensation and methoxylation to give 6-methoxy-2-[[(4-methoxy-3,5-dimethyl-1-oxido-2-pyridinyl)methyl]thio]-1H-benzimidazole. The target compound was formed by deoxidation with phosphorus trichloride and the oxidation with sodium perborate in an overall yield of 48.7%. The structures of the intermediates and omeprazole were confirmed by 1H NMR and MS. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Reference of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole

The Article related to omeprazole preparation, Heterocyclic Compounds (More Than One Hetero Atom): Imidazoles and other aspects.Reference of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 31, 2008, Koneva, E. A.; Khomenko, T. M.; Kurbakova, S. Yu.; Komarova, N. I.; Korchagina, D. V.; Volcho, K. P.; Salakhutdinov, N. F.; Tolstikov, A. G.; Tolstikov, G. A. published an article.Recommanded Product: 73590-85-9 The title of the article was Synthesis of optically active omeprazole by catalysis with vanadyl complexes with chiral Schiff bases. And the article contained the following:

A new method for the preparation of optically active omeprazole I via asym. oxidation of the corresponding sulfide with the use of vanadyl complexes with chiral Schiff bases as the catalysts has been elaborated. The best yields and enantioselectivity of the oxidation were achieved using the complex of VO(acac)2 with ligand II. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Recommanded Product: 73590-85-9

The Article related to enantioselective omeprazole preparation, Heterocyclic Compounds (More Than One Hetero Atom): Imidazoles and other aspects.Recommanded Product: 73590-85-9

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 14, 2016, Tang, Long; Yin, Yue; Feng, Xiaoqing published a patent.Synthetic Route of 41010-50-8 The title of the patent was Method for synthesizing Pirenzepine hydrochloride key intermediate. And the patent contained the following:

The title method comprises the steps of: conducting rearrangement reaction on 1,3-dihydro-3-phenyl-2H-imidazo[4,5-B]pyridine-2-one under the action of an acidic catalyst to generate 5,11-dihydro-6H-pyrido[2,3-B][1,4]benzodiazepine-6-one, extracting with Et acetate, drying with anhydrous magnesium sulfate, and recrystallizing with anhydrous ethanol-petroleum ether mixed solvent. The method has the advantages of simple process and raw materials, little pollution, and high yield (above 99%), and recycles Et acetate and acidic catalyst, so as to save cost and protect environment. The experimental process involved the reaction of 3-Phenyl-1H-imidazo[4,5-b]pyridin-2(3H)-one(cas: 41010-50-8).Synthetic Route of 41010-50-8

The Article related to dihydro pyrido benzodiazepinone preparation, Heterocyclic Compounds (More Than One Hetero Atom): Diazepines and other aspects.Synthetic Route of 41010-50-8

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem