Hyun, Jinho published the artcileMicrostamping on an activated polymer surfaces: Patterning biotin and streptavidin onto common polymeric biomaterials, Synthetic Route of 359860-27-8, the publication is Langmuir (2001), 17(20), 6358-6367, database is CAplus.
Microstamping on an activated polymer surface (MAPS) is a methodol. that enables biomols. to be patterned on polymers with micrometer spatial resolution MAPS combines homogeneous surface derivatization of a polymer to introduce a reactive functional group followed by reactive microcontact printing (μCP) of a biol. ligand of interest, linked to an appropriate reactive group. We demonstrate here that polyethylene, polystyrene, poly(Me methacrylate), and poly(ethylene terephthalate) films can be successfully modified to introduce COOH groups on their surfaces, which can be subsequently patterned by reactive μCP of amine-terminated biotin after derivatization of the COOH groups with pentafluorophenol. XPS and time-of-flight secondary ion mass spectrometry (TOF-SIMS) confirmed the chem. of MAPS at each stage of the derivatization of the polymer surfaces and reactive μCP of biotin. Micropatterned biotin surfaces fabricated by MAPS were patterned with streptavidin by exploiting mol. recognition between biotin and streptavidin. The formation of streptavidin patterns was examined by fluorescence microscopy of Alexa488-labeled streptavidin and by TOF-SIMS imaging of 15N-labeled recombinant streptavidin, bound to biotin patterns. The contrast in the streptavidin micropatterns was optimized by examining the effect of blocking agents and streptavidin incubation time. Maximum contrast was obtained for binding of 0.1 μM streptavidin from a buffer containing 0.02% (volume/volume) Tween 20 detergent for an incubation time of 1 min.
Langmuir published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Synthetic Route of 359860-27-8.
Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem