Analytical method development and validation of Ramipril and Candesartan cilexetil in synthetic mixture was written by Nagar, Akhil;Deore, Sumit;Bendale, Atul;Kakade, Rajanikant;Sonawane, Chetankumar. And the article was included in Innovations in Pharmaceuticals and Pharmacotherapy in 2020.Name: 1-(((Cyclohexyloxy)carbonyl)oxy)ethyl 1-((2′-(2H-tetrazol-5-yl)-[1,1′-biphenyl]-4-yl)methyl)-2-ethoxy-1H-benzo[d]imidazole-7-carboxylate This article mentions the following:
The aim of the study was to develop a rapid, accurate, and precise ratio spectra derivative spectroscopic high-performance liquid chromatog. method and validated for the estimation of Ramipril (RAM) and Candesartan cilexetil (CAN) in synthetic mixture The estimation of RAM, CAN (6μg/mL) was used as a devisor and for the estimation of CAN, RAM (5μg/mL) used as a devisor. The wavelengths selected for quant. estimation were 331 nm for RAM and 231 nm and for CAN. Results: The result for validation shows that linearity of the developed method was 0.9976 and 0.9994 in the range of 5-10μg/mL and 6-16μg/mL for RAM and CAN, resp. Phenomenex C18 column (250 mmx4.6 mm, 5μm particle size) column was used. Acetonitrile:water (0.5% TEA, pH 4.5 adjusted with 10% orthophosphoric acid) (85:15 volume/volume) as a mobile phase, flow rate 1 mL/min and detection carried out at 220 nm. The retention time of RAM and CAN was 3.607 and 5.613 min, resp. Linearity of the developed method was found to be 0.9956 and 0.9974 in the range of 0.5-0.9μg/mL and 1.60-2.88μg/mL for RAM and CAN, resp. From the mentioned results, we can conclude that the developed methods were validated successfully as per the ICH guideline and are accurate, robust, and precise. In the experiment, the researchers used many compounds, for example, 1-(((Cyclohexyloxy)carbonyl)oxy)ethyl 1-((2′-(2H-tetrazol-5-yl)-[1,1′-biphenyl]-4-yl)methyl)-2-ethoxy-1H-benzo[d]imidazole-7-carboxylate (cas: 145040-37-5Name: 1-(((Cyclohexyloxy)carbonyl)oxy)ethyl 1-((2′-(2H-tetrazol-5-yl)-[1,1′-biphenyl]-4-yl)methyl)-2-ethoxy-1H-benzo[d]imidazole-7-carboxylate).
1-(((Cyclohexyloxy)carbonyl)oxy)ethyl 1-((2′-(2H-tetrazol-5-yl)-[1,1′-biphenyl]-4-yl)methyl)-2-ethoxy-1H-benzo[d]imidazole-7-carboxylate (cas: 145040-37-5) belongs to imidazole derivatives. Many natural products, especially alkaloids, contain the imidazole ring. These imidazoles share the 1,3-C3N2 ring but feature varied substituents. Imidazole also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes.Name: 1-(((Cyclohexyloxy)carbonyl)oxy)ethyl 1-((2′-(2H-tetrazol-5-yl)-[1,1′-biphenyl]-4-yl)methyl)-2-ethoxy-1H-benzo[d]imidazole-7-carboxylate
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem