Influence of reactive oxygen species on the enzyme stability and activity in the presence of ionic liquids was written by Attri, Pankaj;Choi, Eun Ha. And the article was included in PLoS One in 2013.Synthetic Route of C4H7ClN2 This article mentions the following:
The authors have examined the effect of ammonium and imidazolium based ionic liquids (ILs) on the stability and activity of proteolytic enzyme α-chymotrypsin (CT) in the presence of cold atm. pressure plasma jet (APPJ). The present work aims to illustrate the state of art implementing the combined action of ILs and APPJ on the enzyme stability and activity. The authors’ CD, fluorescence and enzyme activity results of CT revealed that buffer and all studied ILs {triethylammonium hydrogen sulfate (TEAS) from ammonium family and 1-butyl-3-Me imidazolium chloride ([Bmim][Cl]), 1-methylimidazolium chloride ([Mim][Cl]) from imidazolium family} are notable to act as protective agents against the deleterious action of the APPJ, except triethylammonium dihydrogen phosphate (TEAP) ammonium IL. However, TEAP attenuates strongly the deleterious action of reactive oxygen species (ROS) created by APPJ on native structure of CT. Further, TEAP is able to retain the enzymic activity after APPJ exposure which is absent in all the other systems. This study provides the first combined effect of APPJ and ILs on biomols. that may generate many theor. and exptl. opportunities. Through this methodol., the authors can use both enzyme and plasma simultaneously without affecting the enzyme structure and activity on the material surface; which can prove to be applicable in various fields. In the experiment, the researchers used many compounds, for example, 1-Methyl-1H-imidazol-3-ium chloride (cas: 35487-17-3Synthetic Route of C4H7ClN2).
1-Methyl-1H-imidazol-3-ium chloride (cas: 35487-17-3) belongs to imidazole derivatives. Many natural products, especially alkaloids, contain the imidazole ring. These imidazoles share the 1,3-C3N2 ring but feature varied substituents. Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division.Synthetic Route of C4H7ClN2
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem