《Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement》 was published in International Journal of Biological Macromolecules in 2020. These research results belong to Lan, Biyun; Wu, Junfeng; Li, Na; Pan, Chenglin; Yan, Li; Yang, Chuan; Zhang, Liming; Yang, Liqun; Ren, Meng. Computed Properties of C7H6N4O The article mentions the following:
Gene vectors are important for successful siRNA delivery. Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin resp. and named as G-EDA, G-DETA, A-EDA and A-DETA. The efficiency and safety of these HCPs to deliver siRNA were explored in vitro and in vivo. Our results showed that HCPs could form complexes with siRNA. All HCP/siRNA exhibited negligible cytotoxicity. Compared with A-EDA and A-DETA, G-EDA and G-DETA could carry much more siRNA into cells and then escape from endosomes. The delivery of MMP-9 siRNA (siMMP-9) by G-EDA and G-DETA significantly inhibited MMP-9 in HaCaT cells. Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing. In summary, this study provided an effective and safe approach for siRNA delivery in vitro and in vivo.Di(1H-imidazol-1-yl)methanone(cas: 530-62-1Computed Properties of C7H6N4O) was used in this study.
Di(1H-imidazol-1-yl)methanone(cas: 530-62-1) is a peptide coupling reagent,it is used in the synthesis of peptides. Reacts readily with carboxylic acids to form acyl imidazoles; subsequent reaction with amines to form amides goes smoothly.Computed Properties of C7H6N4O
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem