Ilnytska, Olga; Jeziorek, Maciej; Lai, Kimberly; Altan-Bonnet, Nihal; Dobrowolski, Radek; Storch, Judith published the artcile< Lysobisphosphatidic acid (LBPA) enrichment promotes cholesterol egress via exosomes in Niemann Pick type C1 deficient cells>, Recommanded Product: p-Benzenediacrylanilide, 4′,4′′-di-2-imidazolin-2-yl-, dihydrochloride, the main research area is NPC1 exosome cholesterol egress lysobisphosphatidic acid; Bis(monoacylglycero)phosphate; Cholesterol; Exosomes; Extracellular vesicles; Lysobisphosphatidic acid; Niemann-Pick C disease; Phosphatidylglycerol.
This article demonstrate that NPC1-independent cholesterol egress in PG/LBPA enriched cells occurs, at least in part, via increased exosomal secretion. We probed Western blots of exosome-enriched preparations from the culture supernatants of untreated and PG-treated human primary NPC1 fibroblasts (GM03123), and found that 24 h PG treatment increased the amount of three classical exosomal markers, Alix, Flotillin-1, and CD63, in the exosome fraction by approx. 2- to 5-fold in FBS-free medium. Immunofluorescent imaging of luminal LAMP1, a marker of the endo-lysosomal compartment, in non-permeabilized NPC1 mutant fibroblasts, revealed a 6 to 10-fold increase in LAMP1-pos. organelles near the cell surface at 3 and 6 h of PG incubation, suggesting their exocytosis at early time points after treatment. We also found that the amount of cholesterol is reduced in exosomes released from cells that were first pretreated with GW4869 for 12 h and then incubated with PG in the presence of GW4869 for an addnl. 24 h, relative to cells incubated with PG but not treated with the compound Increase in C18:1,22:6 LBPA species in PG-treated cells may contribute to the formation of ILV, leading to increased exosome secretion and, hence, increased cholesterol secretion. The author concluded that PG treatment of NPC1 deficient cells leads to a reduction in LE/LY cholesterol accumulation, at least in part via exosomal egress.
Biochimica et Biophysica Acta, Molecular and Cell Biology of Lipids published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (Alix). 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Recommanded Product: p-Benzenediacrylanilide, 4′,4′′-di-2-imidazolin-2-yl-, dihydrochloride.
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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem