Recommanded Product: 616-47-7In 2021 ,《Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID19》 was published in Journal of Immunological Methods. The article was written by Lorenzo, Maria A.; Pachon, Diana; Maier, Alexandra; Bermudez, Henry; Losada, Sandra; Toledo, Marilyan; Pujol, Flor H.; Alarcon de Noya, Belkisyole; Noya, Oscar; Serrano, Maria Luisa. The article contains the following contents:
Several diagnostic tools have been developed for clin. and epidemiol. assays. RT-PCR and antigen detection tests are more useful for diagnosis of acute disease, while antibody tests allow the estimation of exposure in the population. Currently, there is an urgent need for the development of diagnostic tests for COVID-19 that can be used for large-scale epidemiol. sampling. Through a comprehensive strategy, potential 16 mer antigenic peptides suited for antibody-based SARS-CoV-2 diagnosis were identified. A systematic scan of the three structural proteins (S,N and M) and the non-structural proteins (ORFs) present in the SARS-CoV-2 virus was conducted through the combination of immunoinformatic methods, peptide SPOT synthesis and an immunoassay with cellulose-bound peptides (Pepscan). The Pepscan filter paper sheets with synthetic peptides were tested against pools of sera of COVID-19 patients. Antibody recognition showed a strong signal for peptides corresponding to the S, N and M proteins of SARS-CoV-2 virus, but not for the ORFs proteins. The peptides exhibiting higher signal intensity were found in the C-terminal region of the N protein. Several peptides of this region showed strong recognition with all three Igs in the pools of sera. The differential reactivity observed between the different Ig isotypes (IgA, IgM and IgG) within different regions of the S and N proteins, can be advantageous for ensuring accurate diagnosis of all infected patients, with different times of exposure to infection. Few peptides of the M protein showed antibody recognition and no recognition was observed for peptides of the ORFs proteins. The results came from multiple reactions, including the reaction of 1-Methyl-1H-imidazole(cas: 616-47-7Recommanded Product: 616-47-7)
1-Methyl-1H-imidazole(cas: 616-47-7) is used as a precursor for the synthesis of pyrrole-imidazole polyamides, ionic liquids such as 1-butyl-3-methylimidazolium hexafluorophosphate.Recommanded Product: 616-47-7
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem