On April 20, 1993, Palejwala, Vaseem A.; Rzepka, Robert W.; Humayun, M. Zafri published an article.Reference of Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was UV irradiation of Escherichia coli modulates mutagenesis at a site-specific ethenocytosine residue on M13 DNA. Evidence for an inducible recA-independent effect. And the article contained the following:
Mutagenic action of chem. and phys. mutagens is mediated through DNA damage and subsequent misreplication at sites of unrepaired damage. Most DNA damage is noninstructive in the sense that the causative chem. modification either destroys the template information or renders it inaccessible to the DNA polymerase. Noninstructive adducts possess high genotoxicity because they stop DNA replication. Replication past noninstructive adducts is thought to depend on induced functions in addition to the regular replication machinery. In E. coli, noninstructive DNA damage leads to induction of the SOS regulon, which in turn is thought to provide the inducible functions required for replicative bypass of the lesion. Because of the absence of accessible template instruction, base incorporation opposite noninstructive lesions is inherently error-prone and results in mutagenesis. Ethenocytosine (εC), an exocyclic DNA lesion induced by carcinogens such as vinyl chloride and urethane, is a highly mutagenic, noninstructive lesion on the basis of its template characteristics in vivo and vitro. However, mutagenesis at εC does not require SOS functions, as evidenced by efficient mutagenesis in recA-deleted E. coli. Even though efficient mutagenesis in recA-deleted cells shows a lack of SOS dependence, the question remains whether SOS induction can modulate mutagenesis opposite εC. To exam. the possible contribution of SOS functions to mutagenesis at εC, an M13 duplex circular DNA mol. containing an εC residue was constructed at a unique site. The construct was transfected into nonirradiated or UV-irradiated E. coli. The frequency as well as specificity of the mutations induced under a number of conditions was determined by using a multiplex DNA sequencing technol. Without prior UV irradiation, ∼33% of the host cells show a significant increase in mutagenesis, with most of the increase accounted for by an increase in C→A transversions. Surprisingly, essentially identical effects were observed in irradiated recA-deleted cells as well as in umuC-deficient cells, suggesting that the observed UV modulation of mutagenesis is independent of the SOS pathway. These observations suggest the existence of a recA-independent UV-inducible mutagenic mechanism in E. coli. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Reference of Imidazo[1,2-c]pyrimidin-5(6H)-one
The Article related to uv mutagenesis dna ethenocytosine site escherichia, Radiation Biochemistry: Effects In Microorganisms and other aspects.Reference of Imidazo[1,2-c]pyrimidin-5(6H)-one
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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem