On September 30, 2001, Mokkapati, Sanath K.; Fernandez de Henestrosa, A. R.; Bhagwat, Ashok S. published an article.Category: imidazoles-derivatives The title of the article was Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells. And the article contained the following:
The Escherichia coli DNA glycosylase Mug (mismatched uracil glycosylase) excises 3,N4-ethenocytosines (εC) and uracils from DNA, but its biol. function is obscure. This is because εC is not found in E. coli DNA, and uracil-DNA glycosylase (Ung), a distinct enzyme, is much more efficient at removing uracils from DNA than Mug. We find that Mug is overexpressed as cells enter stationary phase, and it is maintained at a fairly high level in resting cells. This is true of cells grown in rich or minimal media, and the principal regulation of mug is at the level of mRNA. Although the expression of mug is strongly dependent on the stationary-phase sigma factor, σS, when cells are grown in minimal media, it shows only a modest dependence on σS when cells are grown in rich media. When mug cells are maintained in stationary phase for several days, they acquire many more mutations than their mug+ counterparts. This is true in ung as well as ung+ cells, and a majority of new mutations may not be C to T. Our results show that the biol. role of Mug parallels its expression in cells. It is expressed poorly in exponentially growing cells and has no apparent role in mutation avoidance in these cells. In contrast, Mug is fairly abundant in stationary-phase cells and has an important anti-mutator role at this stage of cell growth. Thus, Mug joins a very small coterie of DNA repair enzymes whose principal function is to avoid mutations in stationary-phase cells. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Category: imidazoles-derivatives
The Article related to dna glycosylase mug stationary phase mutation avoidance, escherichia dna glycosylase mug stationary phase mutation avoidance, gene mug escherichia stationary phase, Biochemical Genetics: Genomic Processes and other aspects.Category: imidazoles-derivatives
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem