Yang, Siqian; Wang, Yaoxin; Chen, Ying; Dai, Qi published an article in 2020, the title of the article was MASQC: next generation sequencing assists third generation sequencing for quality control in N6-methyladenine DNA identification.Formula: C6H7N5 And the article contains the following content:
DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-mol. real-time sequencing (SMRT-seq) can detect 6mA events in prokaryotic and eukaryotic genomes at the single-nucleotide level. However, there are no strict and economical quality control methods for high false-pos. 6mA events in eukaryotic genomes. Therefore, by analyzing the distribution of 6mA in eukaryotic and prokaryotes, we proposed a method named MASQC (MeDIP-seq assists SMRT-seq for quality control in 6mA identification), which can identify 6mA events without doing the whole genome amplification (WGA) sequencing. The proposed MASQC method was assessed on two eukaryotic genomes and six bacterial genomes, our results demonstrate that MASQC performs well in quality control of false pos. 6mA identification for both eukaryotic and prokaryotic genomes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5
The Article related to methyladenine prokaryote eukaryote next generation dna sequencing, dna n6-methyladenine, medip-seq, smrt-seq, eukaryotes, prokaryotes, Biochemical Genetics: Methods and other aspects.Formula: C6H7N5
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