Suwalsky, Mario published the artcileIn vitro effects of benzimidazole/thioether-copper complexes with antitumor activity on human erythrocytes, SDS of cas: 4760-35-4, the publication is Journal of Inorganic Biochemistry (2018), 87-93, database is CAplus and MEDLINE.
Two cytotoxic copper(II) complexes with N-H and N-methylated benzimidazole-derived ligands (Cu-L1 and Cu-L1Me; L1 = bis(2-methylbenzimidazolyl)(2-methylthioethyl)amine, L1Me = bis(1-methyl-2-methylbenzimidazolyl)(2-methylthioethyl)amine) were synthesized and exposed to human erythrocytes and mol. models of its membrane. The latter were bilayers built-up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), classes of lipids present in the external and internal moieties of the human red cell membrane, resp. SEM of erythrocytes incubated with solutions of both Cu(II) complexes showed that they induced morphol. changes to the normal cells to echinocytes, and hemolysis at higher concentrations Real-time observation of the dose-dependent effects of the complexes on live erythrocytes by defocusing microscopy (DM) confirmed SEM results. The formation of echinocytes implied that complex mols. inserted into the outer moiety of the red cell membrane. X-ray diffraction studies on DMPC and DMPE showed that none of these complexes interacted with DMPE and only Cu-L1 interacted with DMPC. This difference was explained by the fact that Cu-L1Me complex is more voluminous than Cu-L1 because it has two addnl. Me groups; on the other hand, DMPC mol. has three Me groups in its bulky terminal amino end. Thus, by steric hindrance Cu-L1Me mols. cannot intercalate into DMPC bilayer, which besides is present in the gel phase. These results, together with the increased antiproliferative capacity of the N-methylated complex Cu-L1Me over that of Cu-L1 are rationalized mainly based on its higher lipophilicity.
Journal of Inorganic Biochemistry published new progress about 4760-35-4. 4760-35-4 belongs to imidazoles-derivatives, auxiliary class Chloride,Benzimidazole, name is 2-(Chloromethyl)-1-methyl-1H-benzo[d]imidazole, and the molecular formula is C7H13ClNNaO5S, SDS of cas: 4760-35-4.
Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem