Ebisu, Keitaro published the artcileN-Terminal Specific Point-Immobilization of Active Proteins by the One-Pot NEXT-A Method, Quality Control of 359860-27-8, the publication is ChemBioChem (2009), 10(15), 2460-2464, database is CAplus and MEDLINE.
The authors earlier combined L/F-transferase-mediated functionalization with tRNA-aminoacylation using engineered aminoacyl-tRNA synthetase (ARS) in the same test tube and named the whole method NEXT-A (N-terminal extension of protein by transferase and aminoacyl-tRNA synthetase). Here, the authors report a practical method that combines the NEXT-A reaction and noncatalytic 1,3-dipolar cycloaddition for immobilizing any kind of active protein onto supports with an ordered orientation. Typically, a sugar-binding protein (lectin EW29Ch) was successfully immobilized onto an acrylamide-based gel. The authors monitored stoichiometry of the reaction during the immobilization. The lectin-immobilized gel was subjected to frontal affinity chromatog. and sugar-lectin interactions on the gel were measured. Direct immobilization of a target protein in a cell lysate without purification was also demonstrated.
ChemBioChem published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Quality Control of 359860-27-8.
Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem