Author: Jessica.F

In 2002,Carter, S. R.; Rimmer, S. published 《Smart hyperbranched polymers for the purification of biomolecules》.Polymer Preprints (American Chemical Society, Division of Polymer Chemistry) published the findings.Product Details of 16681-56-4 The information in the text is summarized as follows:

The use of a SMART polymer such as polyNIPAM allows for a hyperbranched structure to respond to small changes in temperature, pH or ionic strength. A hyperbranched SMART polymer with ligands situated at chain-ends should allow for multi-point attachment to biomacromols. in response to a change in an external stimulus. The hyperbranched poly-NIPAM polymers, where the chain termini are functionalized with an imidazole group, was prepared using the radical addition fragmentation termination (RAFT) methodol. The RAFT technique allows for control over the degree of branching by varying the proportion of dithionate ester to NIPAM. The RAFT agent 4-vinylbenzylimidazole dithioate was synthesized from 4(5)-imidazoledithioic acid and 4-vinylbenzyl chloride in DMF solvent using cesium carbonate as base. The pure product was achieved in moderate yield (27%) by repeated column chromatog. using silica and alumina sorbents. The synthetic procedure developed allowed the production of several series of macro-ligands with various mol. sizes, distances between ligands and spatial arrangement of ligands. The polymers can form multi-point attachments to specific sites on a biomacromol. so that changes in these mol. parameters will be a means of selectively targeting particular biomols. In the experiment, the researchers used 2-Bromo-1H-imidazole(cas: 16681-56-4Product Details of 16681-56-4)

2-Bromo-1H-imidazole(cas: 16681-56-4) is a member of imidazole. Its exclusive structural characteristics with enviable electron-rich features are favorable for imidazole-based fused heterocycles to bind efficiently with an array of enzymes and receptors in biological systems through various weak interactions like hydrogen bonds, ion-dipole, cation-π, π-π stacking, coordination, Van der Waals forces, hydrophobic effects, etc., and therefore they demonstrate widespread bioactivities. Product Details of 16681-56-4

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

In 2019,International Journal of Pharmaceutics (Amsterdam, Netherlands) included an article by Rompicharla, Sri Vishnu Kiran; Kumari, Preeti; Bhatt, Himanshu; Ghosh, Balaram; Biswas, Swati. Reference of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid. The article was titled 《Biotin functionalized PEGylated poly(amidoamine) dendrimer conjugate for active targeting of paclitaxel in cancer》. The information in the text is summarized as follows:

In the current study, we employed poly(amidoamine) (PAMAM) dendrimers of generation 4 (G4) to deliver paclitaxel (PTX), a poorly soluble anti-cancer agent precisely to cancer cells via its conjugation on dendrimer surface. Further, G4 PAMAM has been PEGylated (PEG) and tagged with Biotin, an essential micronutrient for cellular functions, receptors of which are overexpressed in certain cancers. The synthesized multifunctional conjugates were characterized by 1H NMR and zeta potential anal. techniques. In addition, the conjugates were evaluated in vitro in cell monolayers and 3D spheroids of biotin receptor over-expressed A549 cell line (human non-small cell lung cancer). G4 PTX PEG-Biotin conjugate penetrated at significantly higher extent in monolayers as well as spheroids as studied by flow cytometry and confocal microscopy by visualizing the cells at varied depth. The G4 PTX PEG-Biotin conjugate demonstrated higher cytotoxicity compared to free PTX and G4 PTX PEG conjugate as assessed by MTT assay in monolayers and Presto Blue assay in detached spheroidal cells. G4 PTX PEG-Biotin demonstrated significant inhibition of growth of tumor spheroids. Therefore, the newly synthesized biotin anchored PTX-conjugated dendrimer system is promising and could be further explored for efficiently delivering PTX to biotin receptor overexpressed cancers. In addition to this study using 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid, there are many other studies that have used 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5Reference of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid) was used in this study.

5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.Reference of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid The biotin/avidin or biotin/streptavidin interaction is utilized in many labeling and purification schemes.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

《Synthesis and characterization of some heterocyclic compounds and evaluation of antibacterial activity》 was published in International Journal of Research in Pharmaceutical Sciences (Madurai, India) in 2020. These research results belong to Smaysem, Farah; Salim, Ahmed. Synthetic Route of C7H7N3 The article mentions the following:

In this study, heterocyclic compounds with two nitrogen atoms are prepared by reaction of 2-aminobenzimidazole with formic acid to give amide derivative, which underwent reaction with phenylhydrazine to give Ph hydrazone derivative and which further reacts with Et chloroacetate to obtain Et acetate derivative A number of Schiff bases are prepared by reacting 2-aminobenzimidazole with benzaldehyde derivatives Triazine, oxadiazole, triazole, tetrazoles are synthesized via cyclization of the Schiff base derivatives with Et chloroacetate and chloro acetyl chloride, benzoic acid, 4-nitrophenyl azide, sodium azide and Ph azide resp. Some prepared compounds exhibit antibacterial properties. In the experiment, the researchers used 1H-Benzo[d]imidazol-2-amine(cas: 934-32-7Synthetic Route of C7H7N3)

1H-Benzo[d]imidazol-2-amine(cas: 934-32-7) can be used in the hydrolysis of a choline carbonate. It was also used in the synthesis of imidazo[1,2-a]benzimidazoles.Synthetic Route of C7H7N3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Recommanded Product: 934-32-7In 2021 ,《Using sulfate-functionalized Hf-based metal-organic frameworks as a heterogeneous catalyst for solvent-free synthesis of pyrimido[1,2-a]benzimidazoles via one-pot three-component reaction》 was published in Journal of Industrial and Engineering Chemistry (Amsterdam, Netherlands). The article was written by Dinh Dang, Minh-Huy; Ho Thuy Nguyen, Linh; Thi Thu Nguyen, Trang; Xuan Dat Mai, Ngoc; Hoang Tran, Phuong; Le Hoang Doan, Tan. The article contains the following contents:

In this work, a sulfate-functionalized Hf-cluster-based metal-organic framework was prepared via sulfation of a Hf-MOF, named Hf-BTC, constructed by Hf6 clusters and 1,3,5-tricarboxylate linkers. The Hf-BTC-SO4 material was consequently demonstrated to be an efficiently reusable superacid catalyst for a one-pot three-component reaction of pyrimido[1,2-a]benzimidazoles synthesis. The reaction catalyzed by the sulfated Hf-BTC could be carried out under mild and solvent-free conditions and give superior performance in a wide range of substrates. According to detailed investigation, the good catalytic performance of the sulfated-functionalized MOF likely originates from the high-porosity framework and the high active sites of the functionalized clusters. Importantly, the catalyst was easy to recover and reuse the functionalized framework several times with minor changes in catalytic efficiency. The experimental process involved the reaction of 1H-Benzo[d]imidazol-2-amine(cas: 934-32-7Recommanded Product: 934-32-7)

1H-Benzo[d]imidazol-2-amine(cas: 934-32-7) can be used in the hydrolysis of a choline carbonate. It was also used in the synthesis of imidazo[1,2-a]benzimidazoles.Recommanded Product: 934-32-7

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

《Split-TurboID enables contact-dependent proximity labeling in cells》 was published in Proceedings of the National Academy of Sciences of the United States of America in 2020. These research results belong to Cho, Kelvin F.; Branon, Tess C.; Rajeev, Sanjana; Svinkina, Tanya; Udeshi, Namrata D.; Thoudam, Themis; Kwak, Chulhwan; Rhee, Hyun-Woo; Lee, In-Kyu; Carr, Steven A.; Ting, Alice Y.. Application In Synthesis of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid The article mentions the following:

Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the proteomic anal. of subcellular regions difficult or impossible to access by conventional fractionation-based approaches. Yet some cellular regions, such as organelle contact sites, remain out of reach for current PL methods. To address this limitation, we split the enzyme TurboID into two inactive fragments that recombine when driven together by a protein-protein interaction or membrane-membrane apposition. At endoplasmic reticulum-mitochondria contact sites, reconstituted TurboID catalyzed spatially restricted biotinylation, enabling the enrichment and identification of >100 endogenous proteins, including many not previously linked to endoplasmic reticulum-mitochondria contacts. We validated eight candidates by biochem. fractionation and overexpression imaging. Overall, split-TurboID is a versatile tool for conditional and spatially specific proximity labeling in cells. The experimental process involved the reaction of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5Application In Synthesis of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid)

5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.Application In Synthesis of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid And it has been used for blocking endogenous biotin during immunohistology procedures.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Name: 1H-Imidazol-2-amineIn 2020 ,《In vitro selection of ribozyme ligases that use prebiotically plausible 2-aminoimidazole-activated substrates》 was published in Proceedings of the National Academy of Sciences of the United States of America. The article was written by Walton, Travis; Das Gupta, Saurja; Duzdevich, Daniel; Oh, Seung Soo; Szostak, Jack W.. The article contains the following contents:

The hypothesized central role of RNA in the origin of life suggests that RNA propagation predated the advent of complex protein enzymes. A critical step of RNA replication is the template-directed synthesis of a complementary strand. Two exptl. approaches have been extensively explored in the pursuit of demonstrating protein-free RNA synthesis: template-directed nonenzymic RNA polymerization using intrinsically reactive monomers and ribozyme-catalyzed polymerization using more stable substrates such as biol. 5′-triphosphates. Despite significant progress in both approaches in recent years, the assembly and copying of functional RNA sequences under prebiotic conditions remains a challenge. Here, we explore an alternative approach to RNA-templated RNA copying that combines ribozyme catalysis with RNA substrates activated with a prebiotically plausible leaving group, 2-aminoimidazole (2AI). We applied in vitro selection to identify ligase ribozymes that catalyze phosphodiester bond formation between a template-bound primer and a phosphor-imidazolide-activated oligomer. Sequencing revealed the progressive enrichment of 10 abundant sequences from a random sequence pool. Ligase activity was detected in all 10 RNA sequences; all required activation of the ligator with 2AI and generated a 3′-5′ phosphodiester bond. We propose that ribozyme catalysis of phosphodiester bond formation using intrinsically reactive RNA substrates, such as imidazolides, could have been an evolutionary step connecting purely nonenzymic to ribozyme-catalyzed RNA template copying during the origin of life. The experimental process involved the reaction of 1H-Imidazol-2-amine(cas: 7720-39-0Name: 1H-Imidazol-2-amine)

1H-Imidazol-2-amine(cas: 7720-39-0) belongs to anime. Acylation is one of the most important reactions of primary and secondary amines; a hydrogen atom is replaced by an acyl group (a group derived from an acid, such as RCOOH or RSO3H, by removal of ―OH, such as RC(=O)―, RS(O)2―, and so on). Reagents may be acid chlorides (RCOC1, RSO2C1), anhydrides ((RCO)2O), or even esters (RCOOR′); the products are amides of the corresponding acids.Name: 1H-Imidazol-2-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

In 2020,Journal of Materials Chemistry C: Materials for Optical and Electronic Devices included an article by Young, David C.; Tasior, Mariusz; Laurent, Adele D.; Dobrzycki, Lukasz; Cyranski, Michal K.; Tkachenko, Nikolai; Jacquemin, Denis; Gryko, Daniel T.. Application In Synthesis of Imidazo[1,2-a]pyridine-8-carbonitrile. The article was titled 《Photostable orange-red fluorescent unsymmetrical diketopyrrolopyrrole-BF2 hybrids》. The information in the text is summarized as follows:

The straightforward synthesis of structurally unique DPP-BODIPY hybrids was developed using unsym., imidazopyridine substituted diketopyrrolopyrroles (DPPs). These hybrids exhibit a superb combination of photophys. properties including high photostability, good fluorescence quantum yield as well as markedly bathochromically shifted absorption and emission compared to conventional diketopyrrolopyrroles. Increasing the size of the imidazopyridine substituent and/or the electron donating power of the other aryl substituent can further red shift both absorption and emission to reach ∼650 nm for the free-base and ∼700 nm for B-chelates. A strong intramol. H bond is responsible for the small change in geometry between the ground and excited states and hence relatively small differences in photophys. properties upon formation of B-chelates are observed The solvent dependence of the photophys. properties for the free base and DPP-BF2 complexes were studied and show strong fluorescence with long lifetimes in both nonpolar and polar aprotic environments. In addition to this study using Imidazo[1,2-a]pyridine-8-carbonitrile, there are many other studies that have used Imidazo[1,2-a]pyridine-8-carbonitrile(cas: 136117-70-9Application In Synthesis of Imidazo[1,2-a]pyridine-8-carbonitrile) was used in this study.

Imidazo[1,2-a]pyridine-8-carbonitrile(cas: 136117-70-9) belongs to imidazoles.Imidazole rings are also present in imidazole ring alkaloids, which are potential therapeutics for thrombosis, cancer and inflammatory diseases.Application In Synthesis of Imidazo[1,2-a]pyridine-8-carbonitrile Although other azole heterocycles are ubiquitous in a wide range of biologically active natural products, imidazole rings occur predominantly in the natural amino acid histidine.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem