Category: 359860-27-8

Aslan, Kadir published the artcileControlled and Reversible Aggregation of Biotinylated Gold Nanoparticles with Streptavidin, SDS of cas: 359860-27-8, the publication is Journal of Physical Chemistry B (2004), 108(40), 15631-15639, database is CAplus.

Biotinylated gold nanoparticles were prepared by using a two-step surface modification procedure. First, a carboxyl-terminated alkanethiol was chemisorbed onto the surface of gold nanoparticles in the presence of a stabilizing agent. Subsequently, the carboxyl groups were reacted with (+)-biotinyl-3,6,9,-trioxaundecanediamine and 2-(2-aminoethoxy)ethanol. This procedure resulted in stable, ligand-modified gold nanoparticles. Upon interaction with streptavidin, the biotinylated gold nanoparticles aggregated by means of specific biomol. recognition. Their aggregation was studied by optical absorption spectroscopy. Controlled aggregation of biotinylated gold nanoparticles resulted in a shift in the surface plasmon resonance peak and broadening of the absorption spectrum of the nanoparticles. The spectral changes were used to assess the extent of aggregation. Aggregation was found to be dependent on the concentrations of streptavidin, biotinylated gold nanoparticles, and the surface mole fraction of biotin groups on the nanoparticles. Maximum aggregation was observed when 24 nM streptavidin and 0.80 nM biotinylated gold nanoparticles were used. Reversal of nanoparticle aggregation was accomplished by the addition of soluble biotin to the streptavidin-nanoparticle aggregates. Kinetic anal. of the absorbance data showed that streptavidin-induced aggregation of biotinylated gold nanoparticles could be interpreted in terms of a Reaction-Limited Colloidal Aggregation (RLCA) model. This indicates that optical absorption spectroscopy can provide a quant. measurement of the aggregation process.

Journal of Physical Chemistry B published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, SDS of cas: 359860-27-8.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Yu, Hua-Zhong published the artcileReading Disc-Based Bioassays with Standard Computer Drives, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, the publication is Accounts of Chemical Research (2013), 46(2), 258-268, database is CAplus and MEDLINE.

Traditional methods of disease diagnosis are both time-consuming and labor-intensive, and many tests require expensive instrumentation and trained professionals, which restricts their use to biomedical laboratories Because patients can wait several days (even weeks) for the results, the consequences of delayed treatment could be disastrous. Therefore, affordable and simple point-of-care (POC) biosensor devices could fill a diagnostic niche in the clinic or even at home, as personal glucose meters do for diabetics. These devices would allow patients to check their own health conditions and enable physicians to make prompt treatment decisions, which could improve the chances for rapid recovery and cure. Compact disks (CDs) provide inexpensive substrate materials for the preparation of microarray biochips, and conventional computer drives/disk players can be adapted as precise optical reading devices for signal processing. Researchers can employ the polycarbonate (PC) base of a CD as an alternative substrate to glass slides or silicon wafers for the preparation of microanal. devices. Using the characteristic optical phenomena occurring on the metal layer of a CD, researchers can develop biosensors based on advanced spectroscopic readout (interferometry or surface plasmon resonance). If researchers integrate microfluidic functions with CD mechanics, they can control fluid transfer through the spinning motion of the disk, leading to “lab-on-a-CD” devices. Over the last decade, the authors’ laboratory has focused on the construction of POC biosensor devices from off-the-shelf CDs or DVDs and standard computer drives. Besides the initial studies of the suitability of CDs for surface and materials chem. research (fabrication of self-assembled monolayers and oxide nanostructures), an ordinary optical drive, without modification of either the hardware or the software driver, can function as the signal transducing element for reading disk-based bioassays quant. In this Account, the authors first provide a brief introduction to CD-related materials chem. and microfluidics research. Then the authors describe the mild chem. developed in the authors’ laboratory for the preparation of computer-readable biomol. screening assays: photochem. activation of the polycarbonate (PC) disk surface and immobilization and delivery of probe and target biomols. The authors thoroughly discuss the anal. of the mol. recognition events: researchers can “read” these devices quant. with an unmodified optical drive of any personal computer. Finally, and critically, the authors illustrate the authors’ digitized mol. diagnosis approach with three trial systems: DNA hybridization, antibody-antigen binding, and ultrasensitive lead detection with a DNAzyme assay. These examples demonstrate the broad potential of this new anal./diagnostic tool for medical screening, on-site food/water safety testing, and remote environmental monitoring.

Accounts of Chemical Research published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C7H16ClNO2, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Yang, Peng-Yu published the artcileChemical Modification and Organelle-Specific Localization of Orlistat-Like Natural-Product-Based Probes, COA of Formula: C18H34N4O5S, the publication is Chemistry – An Asian Journal (2011), 6(10), 2762-2775, database is CAplus and MEDLINE.

Orlistat, also known as tetrahydrolipstatin (THL), is an FDA-approved anti-obesity drug with potential anti-cancer activity. Previously, we developed a chem. proteomic approach, based on the Orlistat-like probe (I) for large-scale identification of unknown cellular targets of Orlistat in human hepatocytes. In this article, we report the chem. synthesis and biol. evaluation of an expanded set of Orlistat-like compounds, with the intention to further dissect and manipulate potential cellular targets of Orlistat. In doing so, we carried out proteome-wide activity-based profiling and large-scale pull-down/LCMS anal. of these compounds in live HepG2 cells, and successfully identified many putative cellular targets for Orlistat and its structural analogs. By qual. assessing the spectra counts of potential protein hits against each of the seventeen Orlistat analogs, we obtained both common and unique targets of these probes. Our results revealed that subtle structural modifications of Orlistat led to noticeable changes in both the cellular potency and target profiles of the drug. In order to further improve the cellular activity of Orlistat, we successfully applied the well-established AGT/SNAP-tag technol. to our cell-permeable, benzylguanine (BG)-containing Orlistat variant (II). We showed that the drug could be delivered and effectively retained in different sub-cellular organelles of living cells. This strategy may provide a general and highly effective chem. tool for the potential sub-cellular targeting of small mol. drugs.

Chemistry – An Asian Journal published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C11H9NO3, COA of Formula: C18H34N4O5S.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Lee, Bong Soo published the artcileSurface-Initiated, Atom Transfer Radical Polymerization of Oligo(ethylene glycol) Methyl Ether Methacrylate and Subsequent Click Chemistry for Bioconjugation, Quality Control of 359860-27-8, the publication is Biomacromolecules (2007), 8(2), 744-749, database is CAplus and MEDLINE.

Click chem. was applied to polymeric nanobrushes presenting azide groups at the terminal. Specifically, the non-biofouling oligoethylene glycol Me ether methacrylate film was functionalized to achieve maximized biospecific interactions between surface-attached ligands and target mols. with PEG as an inert background.

Biomacromolecules published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Quality Control of 359860-27-8.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Lee, Zee-Won published the artcileSingle cell array of biotinylated cells using surface functionalization and microcontact printing, Category: imidazoles-derivatives, the publication is Chemistry Letters (2005), 34(5), 648-649, database is CAplus.

This paper describes a versatile method for generating single cell arrays on a glass substrate, which could be applicable to any arbitrary cell types, by a combination of surface functionalization, biotinylation of cells, and microcontact printing (μCP).

Chemistry Letters published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Category: imidazoles-derivatives.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Park, Sangjin published the artcileDual Functional, Polymeric Self-Assembled Monolayers as a Facile Platform for Construction of Patterns of Biomolecules, Formula: C18H34N4O5S, the publication is Langmuir (2007), 23(22), 10902-10905, database is CAplus and MEDLINE.

The authors report a facile approach to the construction of patterns of biomols. based on polymeric self-assembled monolayers (pSAMs) that possess dual functions: “bio-reactive (post-functionalizable)” and “bio-inert (anti-biofouling)” properties. To prepare pSAMs on Si/SiO₂ wafers were new random copolymers synthesized by radical polymerization of poly(ethylene glycol) Me ether methacrylate (PEGMA), 3-(trimethoxysilyl)propyl methacrylate (TMSMA), and N-acryloxysuccinimide (NAS), and referred to as poly(TMSMA-r-PEGMA-r-NAS). Poly(TMSMA-r-PEGMA-r-NAS) was designed to play triple roles: “surface-reactive”, “bio-reactive”, and “bio-inert” ones. The pSAMs of poly(TMSMA-r-PEGMA-r-NAS) were formed on Si/SiO₂ wafers with 1 h incubation of the substrates in the polymer solution, which showed approx. a 1 nm-thick film as measured by ellipsometry. After the formation of the pSAMs, the feasibility of the pSAMs as a dual functional surface (bio-inert and bio-reactive properties) was examined The ability of the pSAMs to block nonspecific adsorption of proteins was evaluated against bovine serum albumin as a model protein. High-resolution N(1s) XPS anal. on the protein adsorption revealed that significant reduction up to âˆ?7% was observed compared to the unmodified Si/SiO₂ wafer. In addition, micropatterns of streptavidin with high signal-to-noise ratios were achieved using microcontact printing (μCP) of NH₂-bearing biotin onto the pSAMs of poly(TMSMA-r-PEGMA-r-NAS) on glass slides, which suggests that other biomols. could also be efficiently immobilized onto the pSAMs with high specificity while minimizing nonspecific adsorption. The surface d. of both bio-reactive and anti-biofouling functionality could be tailored by simply changing initial feed ratios of each monomer in the polymer synthesis:different molar ratios of the bio-reactive group (NAS: 33%, 20%, and 10%, resp.) were employed. When micropatterns of streptavidin were constructed, the pSAMs with 33% NAS moiety showed the highest immobilization of the protein. Taken together, the present dual functional, random copolymers may have warrant applications in the field of biosensors and biochips.

Langmuir published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Formula: C18H34N4O5S.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Hyun, Jinho published the artcileFabrication of “smart” protein nanostructures using molecular recognition and dip-pen nanolithography, Application of N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, the publication is PMSE Preprints (2003), 261-262, database is CAplus.

Dip-pen nanolithog. in combination with the high-affinity streptavidin-biotin and streptavidin-iminobiotin protein ligand system provide a simple bottom-up approach to creating smart nanoscale biomol. structures in a step-wise fashion by patterning a self-assembled monolayer of a CO₂H-terminated alkanethiol on a gold substrate, followed by covalent immobilization of a high-affinity, small mol. ligand (biotin) on the nanopatterned self-assembled monolayer and mol. recognition of its protein binding partner, streptavidin from solution The resulting nanopattern provides a universal platform for mol. recognition-mediated protein immobilization. Both irreversible immobilization of a biotin-tagged protein and stimulus-triggered, reversible immobilization of N-hydroxysuccinimidoiminobiotin are presented.

PMSE Preprints published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Application of N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Zhu, Shaoli published the artcileA localized surface plasmon resonance nanosensor based on rhombic Ag nanoparticle array, SDS of cas: 359860-27-8, the publication is Sensors and Actuators, B: Chemical (2008), 134(1), 193-198, database is CAplus.

A localized surface plasmon resonance (LSPR) nanosensor by using a rhombic Ag nanoparticle array is proposed and developed. The LSPR extinction spectra of the antigen or antibody with lower concentration are detected. The obtained results detected from 100 nM target mol. indicate a larger wavelength shift for the rhombic based nanosensor than the triangular based nanosensor. By exptl. detection, we demonstrated that the refractive index sensitivity is 266.2 nm per refractive index unit for Ag rhombic nanoparticle with in-plane width of �40 nm and out-of-plane height of 47 nm.

Sensors and Actuators, B: Chemical published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C6H10F3NO, SDS of cas: 359860-27-8.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Lahann, Joerg published the artcileMicroengineered surfaces for biomedical applications based on a polymer active ester, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, the publication is Polymer Preprints (American Chemical Society, Division of Polymer Chemistry) (2001), 42(2), 113-114, database is CAplus.

A generic method for generating patterns of proteins and/or cells based on reactive coating and spatially directed self-assembly was developed. [2.2]Paracyclophane-4-carboxylic acid pentafluorophenol (I) ester was synthesized via three step synthesis. A reactive coating process was done by chem. vapor deposition polymerization of I to produce a reactive film of poly[p-xylylene carboxylic acid pentafluorophenol ester-co-para-xylylene] on the substrate. Surface patterns of biotin-conjugated human antibody against α₅-integrin were designed by the following procedure: (1) reactive coating process, (2) surface patterning using microcontact printing, and (3) generating cell patterns by layer-by-layer self-assembly on the patterned substrates. The method was demonstrated in patterns of endothelial cells.

Polymer Preprints (American Chemical Society, Division of Polymer Chemistry) published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Blom, Elisabeth published the artcile⁶⁸Ga-Labeling of Biotin Analogues and their Characterization, COA of Formula: C18H34N4O5S, the publication is Bioconjugate Chemistry (2009), 20(6), 1146-1151, database is CAplus and MEDLINE.

Biotin- and ⁶⁸Ga-based tracers have been suggested as tools that could be used to monitor the survival of avidin-coated islets of Langerhans isolated from pancreas and used in transplantation, i.e., to liver. Three biotin analogs with various alkyl and poly(ethylene glycol) (PEG) chains coupled to DOTA were synthesized and labeled with ⁶⁸Ga. The ⁶⁸Ga labeling was studied at room temperature as well as elevated temperature using either conventional or microwave heating. Radioactivity incorporation reached 95% within 5 and 2 min using the, resp., conventional and microwave heating modes. The specific activity of the tracers was improved by preconcentration and purification of the generator eluate. The binding of the labeled and nonlabeled conjugates to avidin in solution was compared to the binding of native biotin. All compounds maintained good affinity for avidin, though introducing the linkers and chelator, especially the PEG-groups, somewhat decreased the binding affinity. The extent of binding of the labeled compounds to avidin was 54-91% after 5 min. Blocking experiments were performed confirming the specificity of the binding of biotin analogs to avidin. The stability of the three labeled compounds in human serum was studied. The stability of the biotin analog 8 (65% within 30 min) and avidin-biotin complex (80% within 120 min) might be sufficient for the monitoring of the islets of Langerhans. The tracers will be evaluated in in vitro experiments of avidin-coated islets of Langerhans and in transplantation models in vivo.

Bioconjugate Chemistry published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, COA of Formula: C18H34N4O5S.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem