Category: 359860-27-8

Lee, Sunmook published the artcileDextran-Gold Nanoparticle Hybrid Material for Biomolecule Immobilization and Detection, HPLC of Formula: 359860-27-8, the publication is Analytical Chemistry (2005), 77(22), 7204-7211, database is CAplus and MEDLINE.

The formation of a hybrid metal-biopolymer material is described. The synthesis of this material consists of functionalizing the surface of gold nanoparticles through a series of steps that lead to epoxy-functionalized nanoparticles. These are subsequently reacted with hydroxyl moieties of the α-D-glucopyranosyl groups of dextran. Subsequently, the dextran chains are carboxylated through treatment with bromoacetic acid. The resultant material combines the unique optical properties of gold nanoparticles with the versatility that carboxylated dextran offers for further functionalization with biomols. The interaction of this material with three proteins was then investigated through changes in the plasmon resonance properties of the gold nanoparticles. Con A, a lectin that binds glucose and mannose by specific mol. recognition, interacts readily with this material and such interaction is easily detected using optical absorption spectroscopy. Through reaction of the carboxyl groups with (+)-biotinyl-3,6,9,-trioxaundecanediamine, a material bearing biotin groups was obtained. This could interact with streptavidin or antibiotin by specific mol. recognition. Further confirmation of biospecific interactions was obtained with control experiments in which the binding sites were blocked through preincubation of the proteins with the corresponding ligand in solution Binding of these proteins was concentration-dependent over a wide concentration range. This material provides a simple and convenient colorimetric method for biospecific interaction anal.

Analytical Chemistry published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, HPLC of Formula: 359860-27-8.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Pavli, P. published the artcileChemical binding of biomolecules to micropatterned epoxy modified surfaces for biosensing applications, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, the publication is Microelectronic Engineering (2009), 86(4-6), 1473-1476, database is CAplus.

The formation of micro/nanostructures that can be used for binding of specific biomols. is of great importance for the development of multi-analyte biosensing devices and high-throughput microarrays. In this paper, we report on the chem. binding of biotin on an epoxy photolithog. patterned surface. In particular, we used a neg. chem. amplified epoxy resist (EPR) in order to create very small structures by photolithog., in which biotin derivatives functionalized with succinimidyl ester or an amine group were immobilized through covalent bonding. Lithog. process with exposure at 248 nm was optimized, and structures with diameter down to 0.5 μm were demonstrated, where biomols. were successfully bound.

Microelectronic Engineering published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Jankowska-Anyszka, Marzena published the artcileSynthesis of a new class of ribose functionalized dinucleotide cap analogs for biophysical studies on interaction of cap-binding proteins with the 5′ end of mRNA, Synthetic Route of 359860-27-8, the publication is Organic & Biomolecular Chemistry (2011), 9(15), 5564-5572, database is CAplus and MEDLINE.

MRNAs of primitive eukaryotes such as Caenorhabditis elegans and Ascaris summ possess two different caps at their 5′ terminus. They have either a typical cap which consists of 7-methylguanosine linked via a 5′,5′-triphosphate bridge to the first transcribed nucleotide (MMG cap) or an atypical hypermethylated form with two addnl. Me groups at the N2 position (TMG cap). Studies on interaction between the 5′ end of mRNA and proteins that specifically recognize its structure have been carried out for several years and they often require chem. modified cap analogs. Here, we present the synthesis of five novel dinucleotide MMG and TMG cap analogs designed for binding studies using biophys. methods such as ESR and surface plasmon resonance. New analogs were prepared by derivatization of the 2′,3′-cis diol of the second nucleotide in the cap structure with levulinic acid, and coupling of the obtained acetal through its carboxylic group with 4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl (4-amino TEMPO), ethylenediamine (EDA) or (+)-biotinyl-3,6,9-trioxaundecanediamine (amine-PEO₃-biotin).

Organic & Biomolecular Chemistry published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Synthetic Route of 359860-27-8.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Borcard, Francoise published the artcileSurface modification of biomaterials for conjugation with human fetal osteoblasts, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, the publication is Chimia (2013), 67(4), 213-217, database is CAplus and MEDLINE.

Surface functionalization of hydroxyapatite (HA) and β-tricalcium phosphate (TCP) bioceramics with chem. ligands containing a pyrrogallol moiety was developed to improve the adhesion of bone cell precursors to the biomaterials. Fast and biocompatible copper-free click reaction with azido-modified human fetal osteoblasts resulted in improved cell binding to both HA and TCP bioceramics, opening the way for using this methodol. in the preparation of cell-engineered bone implants.

Chimia published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Barnhill, Hannah N. published the artcileDual modification of turnip yellow mosaic virus: synthesis of a biotinylated luminescent nanoparticle, Quality Control of 359860-27-8, the publication is PMSE Preprints (2006), 379-380, database is CAplus.

In this study, turnip yellow mosaic virus (TYMV) was employed as a scaffold for creating a bi-functional nanoparticle. TYMV is a 30 nm icosohedral virus with 60 asym. units and 180 protein subunits. As a proof concept that TYMV can be used as a multifunctional nanoparticle, luminescent metal complexes and the bio-active mol. biotin was conjugated onto the surface of TYMV using traditional bioconjugation methods. This study showed that the particle retains its integrity after the reaction and is stable to the attached functional groups. In addition, the two functional groups still retain their properties and can interact with each other on the surface as demonstrated by fluorescence resonance energy transfer.

PMSE Preprints published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Quality Control of 359860-27-8.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Piggott, Andrew M. published the artcileRapid isolation of novel FK506 binding proteins from multiple organisms using gDNA and cDNA T7 phage display, Formula: C18H34N4O5S, the publication is Bioorganic & Medicinal Chemistry (2009), 17(19), 6841-6850, database is CAplus and MEDLINE.

Reverse chem. proteomics using T7 phage display is a powerful technique for identifying cellular receptors of biol. active small mols. However, to date this method has generally been limited to cDNA libraries constructed from mRNA isolated from eukaryotes. In this paper, the authors describe the construction of the first prokaryotic T7 phage display libraries from randomly digested Pseudomonas stutzeri and Vibrio fischeri gDNA, as well as a plant cDNA library from Arabidopsis thaliana. The authors also describe the use of T7 phage display to identify novel proteins from environmental DNA samples using biotinylated FK506 as a model affinity probe.

Bioorganic & Medicinal Chemistry published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Formula: C18H34N4O5S.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Akkahat, Piyaporn published the artcileDevelopment of a Novel Antifouling Platform for Biosensing Probe Immobilization from Methacryloyloxyethyl Phosphorylcholine-Containing Copolymer Brushes, Name: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, the publication is Langmuir (2012), 28(13), 5872-5881, database is CAplus and MEDLINE.

The immobilization of thiol-terminated poly[(methacrylic acid)-ran-(2-methacryloyloxyethyl phosphorylcholine)] (PMAMPC-SH) brushes on gold-coated surface plasmon resonance (SPR) chips was performed using the grafting to approach via self-assembly formation. The copolymer brushes provide both functionalizability and antifouling characteristics, desirable features mandatorily required for the development of an effective platform for probe immobilization in biosensing applications. The carboxyl groups from the methacrylic acid (MA) units were employed for attaching active biomols. that can act as sensing probes for biospecific detection of target mols., whereas the 2-methacryloyloxyethyl phosphorylcholine (MPC) units were introduced to suppress unwanted nonspecific adsorption. The detection efficiency of the biotin-immobilized PMAMPC brushes with the target mol., avidin (AVD), was evaluated in blood plasma in comparison with the conventional 2D monolayer of 11-mercaptoundecanoic acid (MUA) and homopolymer brushes of poly(methacrylic acid) (PMA) also immobilized with biotin using the SPR technique. Copolymer brushes with 79 mol % MPC composition and a mol. weight of 49.3 kDa yielded the platform for probe immobilization with the best performance considering its high S/N ratio as compared with platforms based on MUA and PMA brushes. In addition, the detection limit for detecting AVD in blood plasma solution is 1.5 nM (equivalent to 100 ng/mL). The results demonstrated the potential for using these newly developed surface-attached PMAMPC brushes for probe immobilization and subsequent detection of designated target mols. in complex matrixes such as blood plasma and clin. samples.

Langmuir published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Name: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Jeong, Jae Hyun published the artcileA novel immobilization technique using a poly(amino acid) multilayer designed for surface plasmon resonance sensing, HPLC of Formula: 359860-27-8, the publication is Chemical Physics Letters (2006), 421(4-6), 373-377, database is CAplus.

The novel immobilization technique using a poly(amino acid) multilayer was designed for the SPR sensing platform. The poly(amino acid) multilayer was confirmed as a relatively hydrophilic surface with little tendency for nonspecific adsorption of biomols. and a flat surface that the biomol. interactions can occur uniformly on the sensing surface. The poly(amino acid) multilayer functionalized with biotin was investigated to control the surface d. and to estimate the optimum space between ligands for effective SPR sensing. This multilayer study demonstrates the importance of surface d. which is significantly sensitive for fabrication of surface functional group in the monitoring of kinetic phenomena.

Chemical Physics Letters published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, HPLC of Formula: 359860-27-8.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Lee, Bong Soo published the artcileFormation of activation-free, selectively bioconjugatable poly(N-acryloxysuccinimide-co-oligoethylene glycol methyl ether methacrylate) films by surface-initiated ARGET ATRP, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, the publication is Journal of Polymer Science, Part A: Polymer Chemistry (2017), 55(2), 329-337, database is CAplus.

Activation-free copolymeric films possessing high selectivity to target proteins and low biofouling background are prepared via controlled radical polymerization The copolymeric films are generated by surface-initiated activators regenerated by electron transfer atom transfer radical polymerization (SI-ARGET ATRP) of N-acryloxysuccinimide (NAS) and oligo(ethylene glycol) Me ether methacrylate (OEGMEMA) by controlling the molar feed ratio of the two monomers. The formation of copolymeric films is characterized by ellipsometry, contact angle goniometry, FTIR spectroscopy, and XPS. The prepared copolymeric films are biotinylated without an activation step. Biotin-streptavidin association is employed as a model system to investigate both selective binding and the relevant signal-to-noise (S/N) ratio. When the molar feed ratio of NAS and OEGMEMA is 2:8, the copolymeric film is optimized to give the highest S/N ratio (339.8) according to surface plasmon resonance studies. The highly selective bioconjugation is used to generate micropatterns of rhodamine-conjugated streptavidin on the copolymeric film. © 2016 Wiley Periodicals, Inc.J. Polym. Sci., Part A: Polym.Chem. 2016.

Journal of Polymer Science, Part A: Polymer Chemistry published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C18H34N4O5S, Recommanded Product: N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem

Yu, Hye-Weon published the artcileMultiplex competitive microbead-based flow cytometric immunoassay using quantum dot fluorescent labels, Quality Control of 359860-27-8, the publication is Analytica Chimica Acta (2012), 191-198, database is CAplus and MEDLINE.

The simultaneous anal. of environmental hazards, microcarrier-based multiplex technologies show great promise. Further integration with biofunctionalized quantum dots (QDs) creates new opportunities to extend the capabilities of multicolor flow cytometry with their unique fluorescence properties. We developed a competitive microbead-based flow cytometric immunoassay using QDs fluorescent labels for simultaneous detection of 2 analytes, bringing the benefits of sensitive, rapid and easy-of-manipulation anal. tool for environmental contaminants. As model target compounds, the cyanobacterial toxin microcystin-LR and the polycyclic aromatic hydrocarbon compound benzo[a]pyrene were selected. The assay was carried out in 2 steps: the competitive immunol. reaction of multiple targets using their exclusive sensing elements of QD/antibody detection probes and antigen-coated microsphere, and the subsequent flow cytometric anal. The fluorescence of the QD-encoded microsphere was thus found to be inversely proportional to target analyte concentration Under optimized conditions, the proposed assay performed well within 30 min for the identification and quant. anal. of the 2 contaminants. For microcystin-LR and benzo[a]pyrene, dose-response curves with IC₅₀ values of 5 and 1.1 μg/L and dynamic ranges of 0.52-30 and 0.13-10 μg/L were obtained, resp. Recovery was 92.6-106.5% for 5 types of water samples (bottled water, tap water, surface water and seawater) using only filtration as sample pretreatment.

Analytica Chimica Acta published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C13H9FO, Quality Control of 359860-27-8.

Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem