Category: 443-72-1

Yang, Siqian; Wang, Yaoxin; Chen, Ying; Dai, Qi published an article in 2020, the title of the article was MASQC: next generation sequencing assists third generation sequencing for quality control in N6-methyladenine DNA identification.Formula: C6H7N5 And the article contains the following content:

DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-mol. real-time sequencing (SMRT-seq) can detect 6mA events in prokaryotic and eukaryotic genomes at the single-nucleotide level. However, there are no strict and economical quality control methods for high false-pos. 6mA events in eukaryotic genomes. Therefore, by analyzing the distribution of 6mA in eukaryotic and prokaryotes, we proposed a method named MASQC (MeDIP-seq assists SMRT-seq for quality control in 6mA identification), which can identify 6mA events without doing the whole genome amplification (WGA) sequencing. The proposed MASQC method was assessed on two eukaryotic genomes and six bacterial genomes, our results demonstrate that MASQC performs well in quality control of false pos. 6mA identification for both eukaryotic and prokaryotic genomes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to methyladenine prokaryote eukaryote next generation dna sequencing, dna n6-methyladenine, medip-seq, smrt-seq, eukaryotes, prokaryotes, Biochemical Genetics: Methods and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On June 30, 2020, Spingardi, Paolo; Kriaucionis, Skirmantas published an article.Application of 443-72-1 The title of the article was How m6A sneaks into DNA. And the article contained the following:

A review. The biol. function and origin of N6-methyladenine (m6A) in DNA have been widely debated. A new study demonstrates that the majority of m6A in DNA originates from RNA catabolism via a nucleotide salvage pathway. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to review dna n6 methyladenine rna catabolism nucleotide salvage pathway, General Biochemistry: Reviews and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2021, Liu, Xiaoling; Lai, Weiyi; Li, Yao; Chen, Shaokun; Liu, Baodong; Zhang, Ning; Mo, Jiezhen; Cong, Lyu; Zheng, Jing; Du, Ya-Rui; Jiang, Guibin; Xu, Guo-Liang; Wang, Hailin published an article.Recommanded Product: N-Methyl-7H-purin-6-amine The title of the article was N6-methyladenine is incorporated into mammalian genome by DNA polymerase. And the article contained the following:

AIM: This article discussed about incorporation of N6-methyladenine into mammalian genome by DNA polymerase. To provide robust and reliable data, contamination-free UHPLC-MS/MS technol. was for ultrasensitive and accurate detection of N6-methyladenine in mammals. We measured N6-methyladenine in human embryonic kidney cells, mesenchymal stem cells and embryonic stem cells. Similar levels of DNA 6mA were detected by treating late G1-phase arresting agent L-mimosine. We observed accumulation of genomic 6mA in both mouse and human ES cells and HEK293T cells. By using early G1-phase arresting palbociclib, we observed moderate increase of N6-methyladenine. We exploited unique stable isotope-labeled deoxyadenosine tracing technol. to investigate 6mA origin. UHPLC-MS/MS anal. revealed >67% of genomic deoxyadenosine was labeled in form of [15N4]-deoxyadenosine. Despite efficient labeling of dA, we failed to detect any [15N4]-6mA in three tested cell lines at all cell cycle phases. We observed [15N4]-6mA by transfecting HEK293T cells with plasmid carrying E. coli 6mA methylase mutant gene has activity of 100 times lower than wild type. We used second stable isotope labeling reagent [13CD3] L-methionine. We did not detect any [13CD3]-6mA in the treated mES cells at all cell cycle phases. Results: 6mA is independent of methylases, proving origin of methylase-independent 6mA. Conclusion: N6-methyladenine is incorporated into mammalian genome by DNA polymerase. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: N-Methyl-7H-purin-6-amine

The Article related to n6 methyladenosine dna polymerase genome embryonic kidney cell, Biochemical Genetics: Methods and other aspects.Recommanded Product: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Wan, Qin-Li; Meng, Xiao; Dai, Wenyu; Luo, Zhenhuan; Wang, Chongyang; Fu, Xiaodie; Yang, Jing; Ye, Qunshan; Zhou, Qinghua published an article in 2021, the title of the article was N6 -methyldeoxyadenine and histone methylation mediate transgenerational survival advantages induced by hormetic heat stress.Safety of N-Methyl-7H-purin-6-amine And the article contains the following content:

Environmental stress can induce survival advantages that are passed down to multiple generations, representing an evolutionarily advantageous adaptation at the species level. Using the nematode worm Caenorhabditis elegans as a model, we found that heat shock experienced in either parent could increase the longevity of themselves and up to the fifth generation of descendants. Mechanistic analyses revealed that transcription factor DAF-16/FOXO, heat shock factor HSF-1, and nuclear receptor DAF-12/FXR functioned transgenerationally to implement the hormetic stress response. Histone H3K9me3 methyltransferases SET-25 and SET-32 and DNA N6 -Me methyltransferase DAMT-1 participated in transmitting high-temperature memory across generations. H3K9me3 and N6 -methyladenine could mark heat stress response genes and promote their transcription in progeny to extend life span. We dissected the mechanisms responsible for implementing and transmitting environmental memories in descendants from heat-shocked parents and demonstrated that hormetic stress caused survival benefits could be transmitted to multiple generations through H3K9me3 and N6 -mA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to methyldeoxyadenine histone methylation transgenerational survival hormetic heat stress, General Biochemistry: Other and other aspects.Safety of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 31, 2022, Cai, Jianhua; Xiao, Guobao; Su, Ran published an article.Product Details of 443-72-1 The title of the article was GC6mA-Pred: A deep learning approach to identify DNA N6-methyladenine sites in the rice genome. And the article contained the following:

DNA N6-methyladenine (6mA) is a pivotal DNA modification for various biol. processes. More accurate prediction of 6mA methylation sites plays an irreplaceable part in grasping the internal rationale of related biol. activities. However, the existing prediction methods only extract information from a single dimension, which has some limitations. Therefore, it is very necessary to obtain the information of 6mA sites from different dimensions, so as to establish a reliable prediction method. In this study, a neural network based bioinformatics model named GC6mA-Pred is proposed to predict N6-methyladenine modifications in DNA sequences. GC6mA-Pred extracts significant information from both sequence level and graph level. In the sequence level, GC6mA-Pred uses a three-layer convolution neural network (CNN) model to represent the sequence. In the graph level, GC6mA-Pred employs graph neural network (GNN) method to integrate various information contained in the chem. mol. formula corresponding to DNA sequence. In our newly built dataset, GC6mA-Pred shows better performance than other existing models. The results of comparative experiments have illustrated that GC6mA-Pred is capable of producing a marked effect in accurately identifying DNA 6mA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Product Details of 443-72-1

The Article related to rice genome deep learning dna n6methyladenine, convolution neural network, dna n6-methyladenine, deep learning, graph neural network, Plant Biochemistry: Other and other aspects.Product Details of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Tang, Lipeng; Wei, Xingyan; Li, Tong; Chen, Yi; Dai, Zhenhua; Lu, Chuanjian; Zheng, Guangjuan published an article in 2021, the title of the article was Emerging perspectives of RNA N6-methyladenosine (m6A) modification on immunity and autoimmune diseases.SDS of cas: 443-72-1 And the article contains the following content:

A review. N6-methyladenosine (m6A) modification, the addition of a methylation decoration at the position of N6 of adenosine, is one of the most prevalent modifications among the over 100 known chem. modifications of RNA. Numerous studies have recently characterized that RNA m6A modification functions as a critical post-transcriptional regulator of gene expression through modulating various aspects of RNA metabolism In this review, we will illustrate the current perspectives on the biol. process of m6A methylation. Then we will further summarize the vital modulatory effects of m6A modification on immunity, viral infection, and autoinflammatory disorders. Recent studies suggest that m6A decoration plays an important role in immunity, viral infection, and autoimmune diseases, thereby providing promising biomarkers and therapeutic targets for viral infection and autoimmune disorders. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to review rna methyladenosine immunity autoimmune disease, n6-methyladenosine (m6a), adaptive immunity, autoimmune disease, innate immunity, viral infection, Immunochemistry: Reviews and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Chao, Yinong; Li, Hua-Bing; Zhou, Jing published an article in 2021, the title of the article was Multiple functions of RNA methylation in T cells: a review.Reference of N-Methyl-7H-purin-6-amine And the article contains the following content:

A review. RNA modification represents one of the most ubiquitous mechanisms of epigenetic regulation and plays an essential role in modulating cell proliferation, differentiation, fate determine, and other biol. activities. At present, over 170 types of RNA modification have been discovered in mRNA (mRNA) and noncoding RNA (ncRNA). RNA methylation, as an abundant and widely studied epigenetic modification, is crucial for regulating various physiol. or pathol. states, espectaly immune responses. Considering the biol. significance of T cells as a defense against viral infection and tumor challenge, we will summarize recent findings of how RNA methylation regulates T cell homeostasis and function, discuss the open questions in this rapidly expanding field of RNA modification, and provide the theory basis and potential therapeutic strategies involving targeting of RNA methylation to orchestrate beneficial T cell immune responses. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine

The Article related to review rna methylation immune response, rna methylation, t cell, epigenetics, immune function, m6a, Immunochemistry: Reviews and other aspects.Reference of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On June 15, 2020, Gu, Jing; Xu, Jun; You, Qidong; Guo, Xiaoke published an article.Product Details of 443-72-1 The title of the article was Recent developments of small molecules targeting RNA m6A modulators. And the article contained the following:

A review. N6-methyladenosine (m6A) is the most abundant internal post-transcriptional modification in eukaryotic mRNA. The development of emerging technologies such as m6A-seq, has helped reveal the fundamental role of m6A-RNA in regulation of the mammalian transcriptome. With the identification and advances in the understanding of m6A modulators, the relationship between m6A and human diseases is gradually being revealed. This review summarizes recent progress in the understanding of the role of m6A modulators in human disease and their structural characteristics. We highlight the potential of small-mol. regulators targeting m6A associated proteins as tool mols. and disease treatment options from the medicinal chem. perspective. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Product Details of 443-72-1

The Article related to review n6 methyladenosine small mol regulators epigenetics, epigenetics, n(6)-methyladenosine, small-molecule regulators, Pharmacology: Reviews and other aspects.Product Details of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Tsukiyama, Sho; Hasan, Mehedi Md; Deng, Hong-Wen; Kurata, Hiroyuki published an article in 2022, the title of the article was BERT6mA: prediction of DNA N6-methyladenine site using deep learning-based approaches.Recommanded Product: 443-72-1 And the article contains the following content:

N6-methyladenine (6mA) is associated with important roles in DNA replication, DNA repair, transcription, regulation of gene expression. Several exptl. methods were used to identify DNA modifications. However, these exptl. methods are costly and time-consuming. To detect the 6mA and complement these shortcomings of exptl. methods, we proposed a novel, deep leaning approach called BERT6mA. To compare the BERT6mA with other deep learning approaches, we used the benchmark datasets including 11 species. The BERT6mA presented the highest AUCs in eight species in independent tests. Furthermore, BERT6mA showed higher and comparable performance with the state-of-the-art models while the BERT6mA showed poor performances in a few species with a small sample size. To overcome this issue, pretraining and fine-tuning between two species were applied to the BERT6mA. The pretrained and fine-tuned models on specific species presented higher performances than other models even for the species with a small sample size. In addition to the prediction, we analyzed the attention weights generated by BERT6mA to reveal how the BERT6mA model extracts critical features responsible for the 6mA prediction. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: 443-72-1

The Article related to dna n6 methyladenine deep learning, 6ma modification prediction, bert, cnn, gru, lstm, word2vec, Biochemical Methods: Biological and other aspects.Recommanded Product: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On November 30, 2021, Lv, Hao; Dao, Fu-Ying; Zhang, Dan; Yang, Hui; Lin, Hao published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was Advances in mapping the epigenetic modifications of 5-methylcytosine (5mC), N6-methyladenine (6mA), and N4-methylcytosine (4mC). And the article contained the following:

A review. DNA modification plays a pivotal role in regulating gene expression in cell development. As prevalent markers on DNA, 5-methylcytosine (5mC), N6-methyladenine (6mA), and N4-methylcytosine (4mC) can be recognized by specific methyltransferases, facilitating cellular defense and the versatile regulation of gene expression in eukaryotes and prokaryotes. Recent advances in DNA sequencing technol. have permitted the positions of different modifications to be resolved at the genome-wide scale, which has led to the discovery of several novel insights into the complexity and functions of multiple methylations. In this review, we summarize differences in the various mapping approaches and discuss their pros and cons with respect to their relative read depths, speeds, and costs. We also discuss the development of future sequencing technologies and strategies for improving the detection resolution of current sequencing technologies. Lastly, we speculate on the potentially instrumental role that these sequencing technologies might play in future research. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to review methylcytosine methyladenine epigenetic modification, 5-methylcytosine, dna sequencing technologies, n4-methylcytosine, n6-methyladenine, Biochemical Genetics: Reviews and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem