Category: imidazoles-derivatives

On January 31, 2021, Liu, Xiaoling; Lai, Weiyi; Li, Yao; Chen, Shaokun; Liu, Baodong; Zhang, Ning; Mo, Jiezhen; Cong, Lyu; Zheng, Jing; Du, Ya-Rui; Jiang, Guibin; Xu, Guo-Liang; Wang, Hailin published an article.Recommanded Product: N-Methyl-7H-purin-6-amine The title of the article was N6-methyladenine is incorporated into mammalian genome by DNA polymerase. And the article contained the following:

AIM: This article discussed about incorporation of N6-methyladenine into mammalian genome by DNA polymerase. To provide robust and reliable data, contamination-free UHPLC-MS/MS technol. was for ultrasensitive and accurate detection of N6-methyladenine in mammals. We measured N6-methyladenine in human embryonic kidney cells, mesenchymal stem cells and embryonic stem cells. Similar levels of DNA 6mA were detected by treating late G1-phase arresting agent L-mimosine. We observed accumulation of genomic 6mA in both mouse and human ES cells and HEK293T cells. By using early G1-phase arresting palbociclib, we observed moderate increase of N6-methyladenine. We exploited unique stable isotope-labeled deoxyadenosine tracing technol. to investigate 6mA origin. UHPLC-MS/MS anal. revealed >67% of genomic deoxyadenosine was labeled in form of [15N4]-deoxyadenosine. Despite efficient labeling of dA, we failed to detect any [15N4]-6mA in three tested cell lines at all cell cycle phases. We observed [15N4]-6mA by transfecting HEK293T cells with plasmid carrying E. coli 6mA methylase mutant gene has activity of 100 times lower than wild type. We used second stable isotope labeling reagent [13CD3] L-methionine. We did not detect any [13CD3]-6mA in the treated mES cells at all cell cycle phases. Results: 6mA is independent of methylases, proving origin of methylase-independent 6mA. Conclusion: N6-methyladenine is incorporated into mammalian genome by DNA polymerase. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: N-Methyl-7H-purin-6-amine

The Article related to n6 methyladenosine dna polymerase genome embryonic kidney cell, Biochemical Genetics: Methods and other aspects.Recommanded Product: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On February 20, 2015, Egloff, David; Oleinich, Igor A.; Freisinger, Eva published an article.Related Products of 55662-66-3 The title of the article was Sequence-specific generation of 1,N6-ethenoadenine and 3,N4-ethenocytosine in single-stranded unmodified DNA. And the article contained the following:

DNA lesions such as 1,N6-ethenoadenine (εA) and 3,N4-ethenocytosine (εC) are ubiquitously present in genomes of different organisms and show increasing levels upon exposure to mutagenic substances or under conditions of chronic inflammations and infections. To facilitate investigations of the mutagenic properties and repair mechanisms of etheno-base adducts, access to oligonucleotides bearing these lesions at defined positions is of great advantage. In this study, we report a new synthetic strategy to sequence-specifically generate etheno-adducts in a single-stranded unmodified DNA sequence making use of a DNA-templated approach that positions the alkylating agent close in space to the resp. target base. In contrast to solid-phase synthesis of modified oligonucleotides such DNA-templated methods can be applied to single-stranded nucleic acids of unrestricted lengths. The modular nature of the system allows straightforward adaptation to different sequences. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Related Products of 55662-66-3

The Article related to etheno adenine cytosine generation single stranded dna adduct, Biochemical Genetics: Methods and other aspects.Related Products of 55662-66-3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Wright, Jeremy; Frank, Lesley R.; Bush, Donna; Harrison, George H. published an article in 1983, the title of the article was Evaluation of nitrobenzimidazoles as hypoxic cell radiosensitizers.Safety of 2-Nitro-1H-benzo[d]imidazole And the article contains the following content:

Radiobiol. and pharmacokinetic assays were performed to determine the potential of 2-nitrobenzimidazole (NBI) as a hypoxic cell radiosensitizing agent. As judged by comparing survival curve slopes of Serratia marcescens irradiated under aerated and hypoxic conditions, the NBI enhancement ratio (ER) at 2 mM concentration was 2.4, compared with an O enhancement ratio of 3.3. 2,5-Dinitrobenzimidazole (DNBI) was investigated in vitro; its ER was 3.0 at 4 mM concentration Very poor tissue penetration of DNBI precluded further testing in vivo. Acute toxic signs appeared in C3H/HeJ mice following i.p. injection of NBI at 100 mg/kg. These would be partly attributable to the stress caused by the high pH of the injection vehicle. The LD50 was estimated to be 125-150 mg/kg. Mammary adenocarcinoma tumors grown in the flanks of these mice exhibited maximum NBI levels at 5 min postinjection (i.p.). Peak tumor radiosensitization occurred in the interval of 5-10 min postinjection. The ER for tumor regrowth delay was 2.1 following 50 mg/kg injected into mice 5 min before irradiation Functional evaluation up to 40 days after treatment revealed no evidence of neurol. deficit. The experimental process involved the reaction of 2-Nitro-1H-benzo[d]imidazole(cas: 5709-67-1).Safety of 2-Nitro-1H-benzo[d]imidazole

The Article related to radiosensitization nitrobenzimidazole, Radiation Biochemistry: Other and other aspects.Safety of 2-Nitro-1H-benzo[d]imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On June 18, 2012, Tian, Yongfeng; Hou, Hongwei; Liu, Yong; Hu, Qingyuan; Wang, An published an article.Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was Analytical method for etheno DNA adducts. And the article contained the following:

This review with 66 references is given on the variety of DNA oxidative damages, repair mechanisms, and their etheno-DNA adducts, as well as anal. methods of etheno-DNA adducts, including immunoaffinity chromatog./32P-post-labeling technique(IC-32P), gas chromatog.-mass spectrometer(GC-MS) and liquid chromatog.-tandem mass spectrometry(LC-MS/MS). The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one

The Article related to review biomarker etheno dna adduct oxidative stress, Biochemical Methods: Reviews and other aspects.Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2022, Lin, Qu; Chen, Jun-wei; Yin, Hao; Li, Ming-an; Zhou, Chu-ren; Hao, Tao-fang; Pan, Tao; Wu, Chun; Li, Zheng-ran; Zhu, Duo; Wang, Hao-fan; Huang, Ming-sheng published an article.Synthetic Route of 443-72-1 The title of the article was DNA N6-methyladenine involvement and regulation of hepatocellular carcinoma development. And the article contained the following:

DNA N6-methyladenine (6 mA) is a new type of DNA methylation identified in various eukaryotic cells. However, its alteration and genomic distribution features in hepatocellular carcinoma (HCC) remain elusive. In this study, we found that N6AMT1 overexpression increased HCC cell viability, suppressed apoptosis, and enhanced migration and invasion, whereas ALKBH1 overexpression induced the opposite effects. Further, 23,779 gain-of-6 mA regions and 11,240 loss-of-6 mA regions were differentially identified in HCC tissues. The differential gain and loss of 6 mA regions were considerably enriched in intergenic regions. Moreover, 7% of the differential 6 mA modifications were associated with tumors, with 60 associated with oncogenes and 57 with tumor suppressor genes (TSGs), and 17 were common to oncogenes and TSGs. The candidate genes affected by 6 mA were filtered by gene ontol. (GO) and RNA-seq. Using quant. polymerase chain reaction (qPCR), BCL2 and PARTICL were found to be correlated with DNA 6 mA in certain HCC processes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to dna n6methyladenine regulation hepatocellular carcinoma development, alkbh1, genomic distribution, hepatocellular carcinoma, n6-methyladenine, n6amt1, Biochemical Genetics: Other and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Wan, Qin-Li; Meng, Xiao; Dai, Wenyu; Luo, Zhenhuan; Wang, Chongyang; Fu, Xiaodie; Yang, Jing; Ye, Qunshan; Zhou, Qinghua published an article in 2021, the title of the article was N6 -methyldeoxyadenine and histone methylation mediate transgenerational survival advantages induced by hormetic heat stress.Safety of N-Methyl-7H-purin-6-amine And the article contains the following content:

Environmental stress can induce survival advantages that are passed down to multiple generations, representing an evolutionarily advantageous adaptation at the species level. Using the nematode worm Caenorhabditis elegans as a model, we found that heat shock experienced in either parent could increase the longevity of themselves and up to the fifth generation of descendants. Mechanistic analyses revealed that transcription factor DAF-16/FOXO, heat shock factor HSF-1, and nuclear receptor DAF-12/FXR functioned transgenerationally to implement the hormetic stress response. Histone H3K9me3 methyltransferases SET-25 and SET-32 and DNA N6 -Me methyltransferase DAMT-1 participated in transmitting high-temperature memory across generations. H3K9me3 and N6 -methyladenine could mark heat stress response genes and promote their transcription in progeny to extend life span. We dissected the mechanisms responsible for implementing and transmitting environmental memories in descendants from heat-shocked parents and demonstrated that hormetic stress caused survival benefits could be transmitted to multiple generations through H3K9me3 and N6 -mA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to methyldeoxyadenine histone methylation transgenerational survival hormetic heat stress, General Biochemistry: Other and other aspects.Safety of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 31, 2022, Cai, Jianhua; Xiao, Guobao; Su, Ran published an article.Product Details of 443-72-1 The title of the article was GC6mA-Pred: A deep learning approach to identify DNA N6-methyladenine sites in the rice genome. And the article contained the following:

DNA N6-methyladenine (6mA) is a pivotal DNA modification for various biol. processes. More accurate prediction of 6mA methylation sites plays an irreplaceable part in grasping the internal rationale of related biol. activities. However, the existing prediction methods only extract information from a single dimension, which has some limitations. Therefore, it is very necessary to obtain the information of 6mA sites from different dimensions, so as to establish a reliable prediction method. In this study, a neural network based bioinformatics model named GC6mA-Pred is proposed to predict N6-methyladenine modifications in DNA sequences. GC6mA-Pred extracts significant information from both sequence level and graph level. In the sequence level, GC6mA-Pred uses a three-layer convolution neural network (CNN) model to represent the sequence. In the graph level, GC6mA-Pred employs graph neural network (GNN) method to integrate various information contained in the chem. mol. formula corresponding to DNA sequence. In our newly built dataset, GC6mA-Pred shows better performance than other existing models. The results of comparative experiments have illustrated that GC6mA-Pred is capable of producing a marked effect in accurately identifying DNA 6mA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Product Details of 443-72-1

The Article related to rice genome deep learning dna n6methyladenine, convolution neural network, dna n6-methyladenine, deep learning, graph neural network, Plant Biochemistry: Other and other aspects.Product Details of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Tang, Lipeng; Wei, Xingyan; Li, Tong; Chen, Yi; Dai, Zhenhua; Lu, Chuanjian; Zheng, Guangjuan published an article in 2021, the title of the article was Emerging perspectives of RNA N6-methyladenosine (m6A) modification on immunity and autoimmune diseases.SDS of cas: 443-72-1 And the article contains the following content:

A review. N6-methyladenosine (m6A) modification, the addition of a methylation decoration at the position of N6 of adenosine, is one of the most prevalent modifications among the over 100 known chem. modifications of RNA. Numerous studies have recently characterized that RNA m6A modification functions as a critical post-transcriptional regulator of gene expression through modulating various aspects of RNA metabolism In this review, we will illustrate the current perspectives on the biol. process of m6A methylation. Then we will further summarize the vital modulatory effects of m6A modification on immunity, viral infection, and autoinflammatory disorders. Recent studies suggest that m6A decoration plays an important role in immunity, viral infection, and autoimmune diseases, thereby providing promising biomarkers and therapeutic targets for viral infection and autoimmune disorders. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to review rna methyladenosine immunity autoimmune disease, n6-methyladenosine (m6a), adaptive immunity, autoimmune disease, innate immunity, viral infection, Immunochemistry: Reviews and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On September 10, 2016, Aasberg, Dennis; Nilsson, Mikael; Olsson, Susanne; Samuelsson, Joergen; Svensson, Olof; Klick, Silke; Ennis, Julie; Butterworth, Paul; Watt, Denise; Iliadou, Stavroula; Karlsson, Angelica; Walker, Joanne T.; Arnot, Kate; Ealer, Norb; Hernqvist, Kerstin; Svensson, Karin; Grinell, Ali; Quist, Per-Ola; Karlsson, Anders; Fornstedt, Torgny published an article.COA of Formula: C17H19N3O2S The title of the article was A quality control method enhancement concept-Continual improvement of regulatory approved QC methods. And the article contained the following:

Quality Control methods (QC-methods) play an important role in the overall control strategy for drug manufacturing However, efficient life-cycle management and continual improvement are hindered due to a variety of post-approval variation legislations across territories and a lack of harmonization of the requirements. As a result, many QC-methods fall behind the tech. development. Developing the QC-method in accordance with the Quality by Design guidelines gives the possibility to do continual improvements inside the original Method Operable Design Region (MODR). However, often it is necessary to do changes outside the MODR, e.g. to incorporate new technol. that was not available at the time the original method was development. Here, we present a method enhancement concept which allows minor adjustments, within the same measuring principle, outside the original MODR without interaction with regulatory agencies. The feasibility of the concept is illustrated by a case study of a QC-method based on HPLC, assumed to be developed before the introduction of UHPLC, where the switch from HPLC to UHPLC is necessary as a continual improvement strategy. The concept relies on the assumption that the System Suitability Test (SST) and failure modes are relevant for other conditions outside the MODR as well when the same measuring principle is used. It follows that it should be possible to move outside the MODR as long as the SST has passed. All minor modifications of the original, approved QC-method must be re-validated according to a template given in the original submission and a statistical equivalence should be shown between the original and modified QC-methods. To summarize, revalidation is handled within the pharmaceutical quality control system according to internal change control procedures, but without interaction with regulating agencies. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).COA of Formula: C17H19N3O2S

The Article related to drug manufacture quality control method improvement, continual improvement, hplc, method enhancement concept, method transfer, quality by design, Pharmaceuticals: General and other aspects.COA of Formula: C17H19N3O2S

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Chao, Yinong; Li, Hua-Bing; Zhou, Jing published an article in 2021, the title of the article was Multiple functions of RNA methylation in T cells: a review.Reference of N-Methyl-7H-purin-6-amine And the article contains the following content:

A review. RNA modification represents one of the most ubiquitous mechanisms of epigenetic regulation and plays an essential role in modulating cell proliferation, differentiation, fate determine, and other biol. activities. At present, over 170 types of RNA modification have been discovered in mRNA (mRNA) and noncoding RNA (ncRNA). RNA methylation, as an abundant and widely studied epigenetic modification, is crucial for regulating various physiol. or pathol. states, espectaly immune responses. Considering the biol. significance of T cells as a defense against viral infection and tumor challenge, we will summarize recent findings of how RNA methylation regulates T cell homeostasis and function, discuss the open questions in this rapidly expanding field of RNA modification, and provide the theory basis and potential therapeutic strategies involving targeting of RNA methylation to orchestrate beneficial T cell immune responses. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine

The Article related to review rna methylation immune response, rna methylation, t cell, epigenetics, immune function, m6a, Immunochemistry: Reviews and other aspects.Reference of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem