Tag: 100-200

Yang, Siqian; Wang, Yaoxin; Chen, Ying; Dai, Qi published an article in 2020, the title of the article was MASQC: next generation sequencing assists third generation sequencing for quality control in N6-methyladenine DNA identification.Formula: C6H7N5 And the article contains the following content:

DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-mol. real-time sequencing (SMRT-seq) can detect 6mA events in prokaryotic and eukaryotic genomes at the single-nucleotide level. However, there are no strict and economical quality control methods for high false-pos. 6mA events in eukaryotic genomes. Therefore, by analyzing the distribution of 6mA in eukaryotic and prokaryotes, we proposed a method named MASQC (MeDIP-seq assists SMRT-seq for quality control in 6mA identification), which can identify 6mA events without doing the whole genome amplification (WGA) sequencing. The proposed MASQC method was assessed on two eukaryotic genomes and six bacterial genomes, our results demonstrate that MASQC performs well in quality control of false pos. 6mA identification for both eukaryotic and prokaryotic genomes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to methyladenine prokaryote eukaryote next generation dna sequencing, dna n6-methyladenine, medip-seq, smrt-seq, eukaryotes, prokaryotes, Biochemical Genetics: Methods and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On April 20, 1993, Palejwala, Vaseem A.; Rzepka, Robert W.; Simha, Devendranath; Humayun, M. Zafri published an article.Recommanded Product: 55662-66-3 The title of the article was Quantitative multiplex sequence analysis of mutational hot spots. Frequency and specificity of mutations induced by a site-specific ethenocytosine in M13 viral DNA. And the article contained the following:

An assay is described for determining the frequency and specificity of mutations occurring at hot spots within a population of DNA mols. The procedure consists of (a) annealing the DNA population with a labeled oligonucleotide designed to prime DNA synthesis at the mutational hot spot; (b) DNA elongation in the presence of a single dideoxynucleoside triphosphate together with 1-3 deoxynucleoside triphosphates, and (c) quantitation of all limit elongation products by high-resolution gel electrophoresis followed by autoradiog. and computing densitometry. Derivation of mutational frequency and specificity over a wide range of values is demonstrated for M13 viral DNA mixtures containing defined proportions of wild-type and mutant DNAs, as well as for M13 viral DNA populations obtained by transfection of DNA bearing a defined site-specific ethenocytosine lesion. The assay is shown to yield results similar to those obtained by laborious clone-by-clone sequencing of viral progeny. The method is not affected significantly by several tested variables and appears to be suitable for use as a quant. assay for sequence microheterogeneity at defined positions within DNA populations. Application of the methodol. demonstrates that ethenocytosine, an exocyclic DNA lesion induced by carcinogens such as vinyl chloride and urethane, is a highly efficient mutagenic lesion with a mutational specificity expected for noninstructive lesions. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Recommanded Product: 55662-66-3

The Article related to mutation hot spot multiplex sequence analysis, m13 virus dna mutation ethenocytosine specificity, Biochemical Genetics: Methods and other aspects.Recommanded Product: 55662-66-3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On June 30, 2020, Spingardi, Paolo; Kriaucionis, Skirmantas published an article.Application of 443-72-1 The title of the article was How m6A sneaks into DNA. And the article contained the following:

A review. The biol. function and origin of N6-methyladenine (m6A) in DNA have been widely debated. A new study demonstrates that the majority of m6A in DNA originates from RNA catabolism via a nucleotide salvage pathway. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to review dna n6 methyladenine rna catabolism nucleotide salvage pathway, General Biochemistry: Reviews and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2021, Liu, Xiaoling; Lai, Weiyi; Li, Yao; Chen, Shaokun; Liu, Baodong; Zhang, Ning; Mo, Jiezhen; Cong, Lyu; Zheng, Jing; Du, Ya-Rui; Jiang, Guibin; Xu, Guo-Liang; Wang, Hailin published an article.Recommanded Product: N-Methyl-7H-purin-6-amine The title of the article was N6-methyladenine is incorporated into mammalian genome by DNA polymerase. And the article contained the following:

AIM: This article discussed about incorporation of N6-methyladenine into mammalian genome by DNA polymerase. To provide robust and reliable data, contamination-free UHPLC-MS/MS technol. was for ultrasensitive and accurate detection of N6-methyladenine in mammals. We measured N6-methyladenine in human embryonic kidney cells, mesenchymal stem cells and embryonic stem cells. Similar levels of DNA 6mA were detected by treating late G1-phase arresting agent L-mimosine. We observed accumulation of genomic 6mA in both mouse and human ES cells and HEK293T cells. By using early G1-phase arresting palbociclib, we observed moderate increase of N6-methyladenine. We exploited unique stable isotope-labeled deoxyadenosine tracing technol. to investigate 6mA origin. UHPLC-MS/MS anal. revealed >67% of genomic deoxyadenosine was labeled in form of [15N4]-deoxyadenosine. Despite efficient labeling of dA, we failed to detect any [15N4]-6mA in three tested cell lines at all cell cycle phases. We observed [15N4]-6mA by transfecting HEK293T cells with plasmid carrying E. coli 6mA methylase mutant gene has activity of 100 times lower than wild type. We used second stable isotope labeling reagent [13CD3] L-methionine. We did not detect any [13CD3]-6mA in the treated mES cells at all cell cycle phases. Results: 6mA is independent of methylases, proving origin of methylase-independent 6mA. Conclusion: N6-methyladenine is incorporated into mammalian genome by DNA polymerase. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: N-Methyl-7H-purin-6-amine

The Article related to n6 methyladenosine dna polymerase genome embryonic kidney cell, Biochemical Genetics: Methods and other aspects.Recommanded Product: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On February 20, 2015, Egloff, David; Oleinich, Igor A.; Freisinger, Eva published an article.Related Products of 55662-66-3 The title of the article was Sequence-specific generation of 1,N6-ethenoadenine and 3,N4-ethenocytosine in single-stranded unmodified DNA. And the article contained the following:

DNA lesions such as 1,N6-ethenoadenine (εA) and 3,N4-ethenocytosine (εC) are ubiquitously present in genomes of different organisms and show increasing levels upon exposure to mutagenic substances or under conditions of chronic inflammations and infections. To facilitate investigations of the mutagenic properties and repair mechanisms of etheno-base adducts, access to oligonucleotides bearing these lesions at defined positions is of great advantage. In this study, we report a new synthetic strategy to sequence-specifically generate etheno-adducts in a single-stranded unmodified DNA sequence making use of a DNA-templated approach that positions the alkylating agent close in space to the resp. target base. In contrast to solid-phase synthesis of modified oligonucleotides such DNA-templated methods can be applied to single-stranded nucleic acids of unrestricted lengths. The modular nature of the system allows straightforward adaptation to different sequences. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Related Products of 55662-66-3

The Article related to etheno adenine cytosine generation single stranded dna adduct, Biochemical Genetics: Methods and other aspects.Related Products of 55662-66-3

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Wright, Jeremy; Frank, Lesley R.; Bush, Donna; Harrison, George H. published an article in 1983, the title of the article was Evaluation of nitrobenzimidazoles as hypoxic cell radiosensitizers.Safety of 2-Nitro-1H-benzo[d]imidazole And the article contains the following content:

Radiobiol. and pharmacokinetic assays were performed to determine the potential of 2-nitrobenzimidazole (NBI) as a hypoxic cell radiosensitizing agent. As judged by comparing survival curve slopes of Serratia marcescens irradiated under aerated and hypoxic conditions, the NBI enhancement ratio (ER) at 2 mM concentration was 2.4, compared with an O enhancement ratio of 3.3. 2,5-Dinitrobenzimidazole (DNBI) was investigated in vitro; its ER was 3.0 at 4 mM concentration Very poor tissue penetration of DNBI precluded further testing in vivo. Acute toxic signs appeared in C3H/HeJ mice following i.p. injection of NBI at 100 mg/kg. These would be partly attributable to the stress caused by the high pH of the injection vehicle. The LD50 was estimated to be 125-150 mg/kg. Mammary adenocarcinoma tumors grown in the flanks of these mice exhibited maximum NBI levels at 5 min postinjection (i.p.). Peak tumor radiosensitization occurred in the interval of 5-10 min postinjection. The ER for tumor regrowth delay was 2.1 following 50 mg/kg injected into mice 5 min before irradiation Functional evaluation up to 40 days after treatment revealed no evidence of neurol. deficit. The experimental process involved the reaction of 2-Nitro-1H-benzo[d]imidazole(cas: 5709-67-1).Safety of 2-Nitro-1H-benzo[d]imidazole

The Article related to radiosensitization nitrobenzimidazole, Radiation Biochemistry: Other and other aspects.Safety of 2-Nitro-1H-benzo[d]imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On June 18, 2012, Tian, Yongfeng; Hou, Hongwei; Liu, Yong; Hu, Qingyuan; Wang, An published an article.Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was Analytical method for etheno DNA adducts. And the article contained the following:

This review with 66 references is given on the variety of DNA oxidative damages, repair mechanisms, and their etheno-DNA adducts, as well as anal. methods of etheno-DNA adducts, including immunoaffinity chromatog./32P-post-labeling technique(IC-32P), gas chromatog.-mass spectrometer(GC-MS) and liquid chromatog.-tandem mass spectrometry(LC-MS/MS). The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one

The Article related to review biomarker etheno dna adduct oxidative stress, Biochemical Methods: Reviews and other aspects.Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2022, Lin, Qu; Chen, Jun-wei; Yin, Hao; Li, Ming-an; Zhou, Chu-ren; Hao, Tao-fang; Pan, Tao; Wu, Chun; Li, Zheng-ran; Zhu, Duo; Wang, Hao-fan; Huang, Ming-sheng published an article.Synthetic Route of 443-72-1 The title of the article was DNA N6-methyladenine involvement and regulation of hepatocellular carcinoma development. And the article contained the following:

DNA N6-methyladenine (6 mA) is a new type of DNA methylation identified in various eukaryotic cells. However, its alteration and genomic distribution features in hepatocellular carcinoma (HCC) remain elusive. In this study, we found that N6AMT1 overexpression increased HCC cell viability, suppressed apoptosis, and enhanced migration and invasion, whereas ALKBH1 overexpression induced the opposite effects. Further, 23,779 gain-of-6 mA regions and 11,240 loss-of-6 mA regions were differentially identified in HCC tissues. The differential gain and loss of 6 mA regions were considerably enriched in intergenic regions. Moreover, 7% of the differential 6 mA modifications were associated with tumors, with 60 associated with oncogenes and 57 with tumor suppressor genes (TSGs), and 17 were common to oncogenes and TSGs. The candidate genes affected by 6 mA were filtered by gene ontol. (GO) and RNA-seq. Using quant. polymerase chain reaction (qPCR), BCL2 and PARTICL were found to be correlated with DNA 6 mA in certain HCC processes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to dna n6methyladenine regulation hepatocellular carcinoma development, alkbh1, genomic distribution, hepatocellular carcinoma, n6-methyladenine, n6amt1, Biochemical Genetics: Other and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Wan, Qin-Li; Meng, Xiao; Dai, Wenyu; Luo, Zhenhuan; Wang, Chongyang; Fu, Xiaodie; Yang, Jing; Ye, Qunshan; Zhou, Qinghua published an article in 2021, the title of the article was N6 -methyldeoxyadenine and histone methylation mediate transgenerational survival advantages induced by hormetic heat stress.Safety of N-Methyl-7H-purin-6-amine And the article contains the following content:

Environmental stress can induce survival advantages that are passed down to multiple generations, representing an evolutionarily advantageous adaptation at the species level. Using the nematode worm Caenorhabditis elegans as a model, we found that heat shock experienced in either parent could increase the longevity of themselves and up to the fifth generation of descendants. Mechanistic analyses revealed that transcription factor DAF-16/FOXO, heat shock factor HSF-1, and nuclear receptor DAF-12/FXR functioned transgenerationally to implement the hormetic stress response. Histone H3K9me3 methyltransferases SET-25 and SET-32 and DNA N6 -Me methyltransferase DAMT-1 participated in transmitting high-temperature memory across generations. H3K9me3 and N6 -methyladenine could mark heat stress response genes and promote their transcription in progeny to extend life span. We dissected the mechanisms responsible for implementing and transmitting environmental memories in descendants from heat-shocked parents and demonstrated that hormetic stress caused survival benefits could be transmitted to multiple generations through H3K9me3 and N6 -mA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to methyldeoxyadenine histone methylation transgenerational survival hormetic heat stress, General Biochemistry: Other and other aspects.Safety of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 31, 2022, Cai, Jianhua; Xiao, Guobao; Su, Ran published an article.Product Details of 443-72-1 The title of the article was GC6mA-Pred: A deep learning approach to identify DNA N6-methyladenine sites in the rice genome. And the article contained the following:

DNA N6-methyladenine (6mA) is a pivotal DNA modification for various biol. processes. More accurate prediction of 6mA methylation sites plays an irreplaceable part in grasping the internal rationale of related biol. activities. However, the existing prediction methods only extract information from a single dimension, which has some limitations. Therefore, it is very necessary to obtain the information of 6mA sites from different dimensions, so as to establish a reliable prediction method. In this study, a neural network based bioinformatics model named GC6mA-Pred is proposed to predict N6-methyladenine modifications in DNA sequences. GC6mA-Pred extracts significant information from both sequence level and graph level. In the sequence level, GC6mA-Pred uses a three-layer convolution neural network (CNN) model to represent the sequence. In the graph level, GC6mA-Pred employs graph neural network (GNN) method to integrate various information contained in the chem. mol. formula corresponding to DNA sequence. In our newly built dataset, GC6mA-Pred shows better performance than other existing models. The results of comparative experiments have illustrated that GC6mA-Pred is capable of producing a marked effect in accurately identifying DNA 6mA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Product Details of 443-72-1

The Article related to rice genome deep learning dna n6methyladenine, convolution neural network, dna n6-methyladenine, deep learning, graph neural network, Plant Biochemistry: Other and other aspects.Product Details of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem