Tag: 100-200

Fernandes, Sara B.; Grova, Nathalie; Roth, Sarah; Duca, Radu Corneliu; Godderis, Lode; Guebels, Pauline; Meriaux, Sophie B.; Lumley, Andrew I.; Bouillaud-Kremarik, Pascaline; Ernens, Isabelle; Devaux, Yvan; Schroeder, Henri; Turner, Jonathan D. published an article in 2021, the title of the article was N6-methyladenine in eukaryotic DNA: tissue distribution, early embryo development, and neuronal toxicity.Product Details of 443-72-1 And the article contains the following content:

DNA methylation is one of the most important epigenetic modifications and is closely related with several biol. processes such as regulation of gene transcription and the development of non-malignant diseases. The prevailing dogma states that DNA methylation in eukaryotes occurs essentially through 5-methylcytosine (5mC) but recently adenine methylation was also found to be present in eukaryotes. In mouse embryonic stem cells, 6-methyladenine (6mA) was associated with the repression and silencing of genes, particularly in the X-chromosome, known to play an important role in cell fate determination Here, we have demonstrated that 6mA is a ubiquitous eukaryotic epigenetic modification that is put in place during epigenetically sensitive periods such as embryogenesis and fetal development. In somatic cells there are clear tissue specificity in 6mA levels, with the highest 6mA levels being observed in the brain. In zebrafish, during the first 120 h of embryo development, from a single pluripotent cell to an almost fully formed individual, 6mA levels steadily increase. An identical pattern was observed over embryonic days 7-21 in the mouse. Furthermore, exposure to a neurotoxic environmental pollutant during the same early life period may led to a decrease in the levels of this modification in female rats. The identification of the periods during which 6mA epigenetic marks are put in place increases our understanding of this mammalian epigenetic modification, and raises the possibility that it may be associated with developmental processes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Product Details of 443-72-1

The Article related to methyladenine eukaryotic dna early embryo development neuronal toxicity, 6-methyladenine, dna methylation, brain, developmental neurotoxicity, embryo development, stress, Pharmaceuticals: Pharmaceutics and other aspects.Product Details of 443-72-1

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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2022, Hui, Wenyan; Zhang, Wenyi; Li, Jing; Kwok, Lai-Yu; Zhang, Heping; Kong, Jian; Sun, Tiansong published an article.HPLC of Formula: 443-72-1 The title of the article was Functional analysis of the second methyltransferase in the bacteriophage exclusion system of Lactobacillus casei Zhang. And the article contained the following:

The antiphage ability is an important feature of fermentation strains in the dairy industry. Our previous work described the bacteriophage exclusion (BREX) system in the probiotic strain, Lactobacillus casei Zhang. The function of L. casei Zhang pglX gene in mediating 5′-ACRCm6AG-3′ methylation was also confirmed. This study aimed to further dissect the function of the BREX system of L. casei Zhang by inactivating its second methyltransferase gene (LCAZH_2054). The methylome of the mutant, L. casei Zhang Δ2054, was profiled by single-mol. real-time sequencing. Then, the cell morphol., growth, plasmid transformation efficiency, and stability of the wildtype and mutant were compared. The mutant did not have an observable effect in microscopic and colony morphol., but it reached a higher cell d. after entering the exponential phase without obvious increase in the cell viability. The mutant had fewer 5′-ACRCm6AG-3′ methylation compared with the wildtype (1835 vs. 1906). Interestingly, no significant difference was observed in the transformation efficiency between the 2 strains when plasmids without cognate recognition sequence (pSec:Leiss:Nuc and pG+host9) were transformed, contrasting to transforming cells with cognate recognition sequence-containing plasmids (pMSP3535 and pTRKH2). The efficiency of transforming pMSP3535 into the LCAZH_2054 mutant was significantly lower than the wildtype, whereas an opposite trend was seen in pTRKH2 transformation. Moreover, compared with the wildtype, the mutant strain had higher capacity in retaining pMSP3535 and lower capacity in retaining pTRKH2, suggesting an unequal tolerance level to different foreign DNA. In conclusion, LCAZH_2054 was not directly responsible for 5′-ACRCm6AG-3′ methylation in L. casei Zhang, but it might help regulate the function and specificity of the BREX system. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1

The Article related to methyltransferase bacteriophage lactobacillus, lactobacillus casei zhang, bacteriophage exclusion system, methyltransferase, phage infection, transformation efficiency, Food and Feed Chemistry: Other and other aspects.HPLC of Formula: 443-72-1

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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On May 31, 2020, Hasan, Mehedi Md.; Manavalan, Balachandran; Shoombuatong, Watshara; Khatun, Mst. Shamima; Kurata, Hiroyuki published an article.Name: N-Methyl-7H-purin-6-amine The title of the article was i6mA-Fuse: improved and robust prediction of DNA 6 mA sites in the Rosaceae genome by fusing multiple feature representation. And the article contained the following:

DNA N6-methyladenine (6 mA) is one of the most vital epigenetic modifications and involved in controlling the various gene expression levels. With the avalanche of DNA sequences generated in numerous databases, the accurate identification of 6 mA plays an essential role for understanding mol. mechanisms. Because the exptl. approaches are time-consuming and costly, it is desirable to develop a computation model for rapidly and accurately identifying 6 mA. To the best of our knowledge, we first proposed a computational model named i6mA-Fuse to predict 6 mA sites from the Rosaceae genomes, especially in Rosa chinensis and Fragaria vesca. We implemented the five encoding schemes, i.e., mononucleotide binary, dinucleotide binary, k-space spectral nucleotide, k-mer, and electron-ion interaction pseudo potential compositions, to build the five, single-encoding random forest (RF) models. The i6mA-Fuse uses a linear regression model to combine the predicted probability scores of the five, single encoding-based RF models. The resultant species-specific i6mA-Fuse achieved remarkably high performances with AUCs of 0.982 and 0.978 and with MCCs of 0.869 and 0.858 on the independent datasets of Rosa chinensis and Fragaria vesca, resp. In the F. vesca-specific i6mA-Fuse, the MBE and EIIP contributed to 75% and 25% of the total prediction; in the R. chinensis-specific i6mA-Fuse, Kmer, MBE, and EIIP contribute to 15%, 65%, and 20% of the total prediction. To assist high-throughput prediction for DNA 6 mA identification, the i6mA-Fuse is publicly accessible at https://kurata14.bio.kyutech.ac.jp/i6mA-Fuse/. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to methyladenine fuse dna genome rosa fragaria, dna 6 ma, feature encoding, machine learning, sequence analysis, Biochemical Methods: Apparatus and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 1, 2020, Wu, Kou-Juey published an article.Computed Properties of 443-72-1 The title of the article was The epigenetic roles of DNA N6-Methyladenine (6mA) modification in eukaryotes. And the article contained the following:

A review. The DNA N6-methyladenine (6mA) modification is a prevalent epigenetic mark in prokaryotes, but the low abundance of 6mA in eukaryotes has recently received attention. The possible role of 6mA as an epigenetic mark in eukaryotes is starting to be recognized. This review article addresses the epigenetic roles of 6mA in eukaryotes. The existence of 6mA in metazoans and plants, the correlation of 6mA with gene expression, the enzymes catalyzing the deposition and removal of the 6mA modification, the relationship of 6mA to nucleosome positioning, the 6mA interaction with chromatin, its role in tumorigenesis and other physiol. conditions/diseases and tech. issues in 6mA detection/profiling and bioinformatics anal. are described. New directions and unresolved issues (e.g., the base-pair-resolution 6mA-sequencing method and gene activation vs. repression) in 6mA research are discussed. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Computed Properties of 443-72-1

The Article related to review epigenetic mark n6methyladenine tumorigenesis, chromatin, demethylase, epigenetic mark, gene expression, methyltransferase, nucleosome positioning, Biochemical Genetics: Reviews and other aspects.Computed Properties of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Bochtler, Matthias; Fernandes, Humberto published an article in 2021, the title of the article was DNA adenine methylation in eukaryotes: Enzymatic mark or a form of DNA damage.SDS of cas: 443-72-1 And the article contains the following content:

A Review. 6-Methyladenine (6mA) is fairly abundant in nuclear DNA of basal fungi, ciliates and green algae. In these organisms, 6mA is maintained near transcription start sites in ApT context by a parental-strand instruction dependent maintenance methyltransferase and is pos. associated with transcription. In animals and plants, 6mA levels are high only in organellar DNA. The 6mA levels in nuclear DNA are very low. They are attributable to nucleotide salvage and the activity of otherwise mitochondrial METTL4, and may be considered as a price that cells pay for adenine methylation in RNA and/or organellar DNA. Cells minimize this price by sanitizing dNTP pools to limit 6mA incorporation, and by converting 6mA that has been incorporated into DNA back to adenine. Hence, 6mA in nuclear DNA should be described as an epigenetic mark only in basal fungi, ciliates and green algae, but not in animals and plants. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to review dna adenine methylation eukaryotes, 6ma, dna damage, dna modifications, cancer, epitranscriptome/epigenome, nucleotide salvage, transcription, Biochemical Genetics: Reviews and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Li, Xuwen; Guo, Shiyuan; Cui, Yan; Zhang, Zijian; Luo, Xinlong; Angelova, Margarita T.; Landweber, Laura F.; Wang, Yinsheng; Wu, Tao P. published an article.Synthetic Route of 443-72-1 The title of the article was NT-seq: a chemical-based sequencing method for genomic methylome profiling. And the article contained the following:

Abstract: DNA methylation plays vital roles in both prokaryotes and eukaryotes. There are three forms of DNA methylation in prokaryotes: N6-methyladenine (6mA), N4-methylcytosine (4mC), and 5-methylcytosine (5mC). Although many sequencing methods have been developed to sequence specific types of methylation, few technologies can be used for efficiently mapping multiple types of methylation. Here, we present NT-seq for mapping all three types of methylation simultaneously. NT-seq reliably detects all known methylation motifs in two bacterial genomes and can be used for identifying de novo methylation motifs. NT-seq provides a simple and efficient solution for detecting multiple types of DNA methylation. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to escherichia helicobacter nt seq genome methylome dna methylation, dna methylation, next-generation sequencing, whole-genome epigenetic profiling, Biochemical Genetics: Methods and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On October 1, 1979, Slaughter, Robert S.; Barnes, Eugene M. Jr. published an article.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was Hypoxanthine transport by Chinese hamster lung fibroblasts: kinetics and inhibition by nucleosides. And the article contained the following:

The transport of 3H-labeled hypoxanthine (I) was studied in monolayer cultures of mutant Chinese hamster lung fibroblasts lacking guanine phosphoribosyltransferase. Initial rates of transport were determined by rapid uptake experiments (8-20 s); a Michaelis constant of 0.68 mM for I was derived from linear, monophasic plots of v/S against v. Nucleosides are competitive inhibitors of this process; adenosine and thymidine give resp. Ki values of 86 and 300 μM. The corresponding bases give much higher inhibition constants with adenine and thymine yielding values of 3100 and 1700 μM, resp. A similar pattern was observed for competitive inhibition of I transport by inosine, adenine arabinoside, uridine, cytidine, and 2 ribofuranosylimidazo derivatives of pyrimidin-4-one; in every case the nucleoside exhibited a lower Ki value than the corresponding homologous base. The inhibition constants observed for nucleosides are remarkably similar to their Km values for nucleoside transport by cultured cells recently reported. I transport was also blocked by the 6-(2-hydroxy-5-nitrobenzylthio) derivatives of inosine and guanosine and by dipyridamole; these agents are also inhibitors of nucleoside transport. These results indicate a closer relation between base and nucleoside transport than previously recognized and suggest that these 2 transport processes may involve identical or very similar transport proteins. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one

The Article related to hypoxanthine fibroblast transport determination, biol transport hypoxanthine isotope determination, nucleoside inhibition hypoxanthine transport, Biochemical Methods: Isotopic and other aspects.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Lowe, Paige; Olinski, Ryszard; Ruzov, Alexey published an article in 2021, the title of the article was Evidence for Noncytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overview.Name: N-Methyl-7H-purin-6-amine And the article contains the following content:

A review. Cytosine DNA methylation (5-methylcytsone, 5mC) is the major DNA modification found in the genomes of animals and plants. Although the roles of 5mC and its oxidized derivatives in the regulation of gene expression are relatively well attested and extensively explored, a number of recent studies imply that noncytosine DNA modifications may also convey specific biol. functions and act as “epigenetic” marks in multicellular organisms. Here we review exptl. evidence for the presence of noncytosine epigenetic modifications in metazoans and plants focusing on two “unusual” DNA bases, 5-hydroxymethyluracil (5hmU) and N6-methyladenine (6mA), and suggest potential explanations for inconsistencies in the currently available data on abundance and potential biol. roles of these DNA modifications in mammals. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to review multicellular eukaryote noncytosine epigenetic dna modification, 5-hydroxymethyluracil, dna modifications, epigenetics, n6-methyladenine, Biochemical Genetics: Reviews and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On November 30, 2021, Lv, Hao; Dao, Fu-Ying; Zhang, Dan; Yang, Hui; Lin, Hao published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was Advances in mapping the epigenetic modifications of 5-methylcytosine (5mC), N6-methyladenine (6mA), and N4-methylcytosine (4mC). And the article contained the following:

A review. DNA modification plays a pivotal role in regulating gene expression in cell development. As prevalent markers on DNA, 5-methylcytosine (5mC), N6-methyladenine (6mA), and N4-methylcytosine (4mC) can be recognized by specific methyltransferases, facilitating cellular defense and the versatile regulation of gene expression in eukaryotes and prokaryotes. Recent advances in DNA sequencing technol. have permitted the positions of different modifications to be resolved at the genome-wide scale, which has led to the discovery of several novel insights into the complexity and functions of multiple methylations. In this review, we summarize differences in the various mapping approaches and discuss their pros and cons with respect to their relative read depths, speeds, and costs. We also discuss the development of future sequencing technologies and strategies for improving the detection resolution of current sequencing technologies. Lastly, we speculate on the potentially instrumental role that these sequencing technologies might play in future research. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to review methylcytosine methyladenine epigenetic modification, 5-methylcytosine, dna sequencing technologies, n4-methylcytosine, n6-methyladenine, Biochemical Genetics: Reviews and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On July 23, 2020, Li, Zheng; Zhao, Shuai; Nelakanti, Raman V.; Lin, Kaixuan; Wu, Tao P.; Alderman, Myles H. III; Guo, Cheng; Wang, Pengcheng; Zhang, Min; Min, Wang; Jiang, Zongliang; Wang, Yinsheng; Li, Haitao; Xiao, Andrew Z. published an article.COA of Formula: C6H7N5 The title of the article was N6-methyladenine in DNA antagonizes SATB1 in early development. And the article contained the following:

Abstract: The recent discovery of N6-methyladenine (N6-mA) in mammalian genomes suggests that it may serve as an epigenetic regulatory mechanism1. However, the biol. role of N6-mA and the mol. pathways that exert its function remain unclear. Here we show that N6-mA has a key role in changing the epigenetic landscape during cell fate transitions in early development. We found that N6-mA is upregulated during the development of mouse trophoblast stem cells, specifically at regions of stress-induced DNA double helix destabilization (SIDD)2-4. Regions of SIDD are conducive to topol. stress-induced unpairing of the double helix and have critical roles in organizing large-scale chromatin structures3,5,6. We show that the presence of N6-mA reduces the in vitro interactions by more than 500-fold between SIDD and SATB1, a crucial chromatin organizer that interacts with SIDD regions. Deposition of N6-mA also antagonizes SATB1 function in vivo by preventing its binding to chromatin. Concordantly, N6-mA functions at the boundaries between euchromatin and heterochromatin to restrict the spread of euchromatin. Repression of SIDD-SATB1 interactions mediated by N6-mA is essential for gene regulation during trophoblast development in cell culture models and in vivo. Overall, our findings demonstrate an unexpected mol. mechanism for N6-mA function via SATB1, and reveal connections between DNA modification, DNA secondary structures and large chromatin domains in early embryonic development. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).COA of Formula: C6H7N5

The Article related to methyladenine dna methylation satb1 embryogenesis mouse trophoblast, Biochemical Genetics: Genomic Processes and other aspects.COA of Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem