Tag: 100-200

Yuan, De-Hui; Xing, Jian-Feng; Luan, Mei-Wei; Ji, Kai-Kai; Guo, Jun; Xie, Shang-Qian; Zhang, Yuan-Ming published an article in 2020, the title of the article was DNA N6-methyladenine modification in wild and cultivated soybeans reveals different patterns in nucleus and cytoplasm.Computed Properties of 443-72-1 And the article contains the following content:

DNA 6mA modification, an important newly discovered epigenetic mark, plays a crucial role in organisms and has been attracting more and more attention in recent years. The soybean is economically the most important bean in the world, providing vegetable protein for millions of people. However, the distribution pattern and function of 6mA in soybean are still unknown. In this study, we decoded 6mA modification to single-nucleotide resolution in wild and cultivated soybeans, and compared the 6mA differences between cytoplasmic and nuclear genomes and between wild and cultivated soybeans. The motif of 6mA in the nuclear genome was conserved across the two kinds of soybeans, and ANHGA was the most dominant motif in wild and cultivated soybeans. Genes with 6mA modification in the nucleus had higher expression than those without modification. Interestingly, 6mA distribution patterns in cytoplasm for each soybean were significantly different from those in nucleus, which was reported for the first time in soybean. Our research provides a new insight in the deep anal. of cytoplasmic genomic DNA modification in plants. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Computed Properties of 443-72-1

The Article related to glycine genomic dna n6methyladenine nucleus cytoplasm, cultivated soybean, cytoplasm, methylation, nucleus, wild soybean, Plant Biochemistry: Classical Genetics and Phylogeny and other aspects.Computed Properties of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 31, 2022, Shi, Hua; Li, Shuang; Su, Xi published an article.HPLC of Formula: 443-72-1 The title of the article was Plant6mA: A predictor for predicting N6-methyladenine sites with lightweight structure in plant genomes. And the article contained the following:

N6-methyladenine (6mA) in DNA, a type of DNA methylation in epigenetic modification, has attracted extensive attention in recent years. In order to improve our understanding of 6mA biol. activities and mechanisms in plant genomes, we need to be able to accurately identify 6mA sites. Because traditional wet-lab experiments frequently necessitate a large amount of manpower and time, a plethora of computational methods, particularly machine learning, have emerged to achieve fast and accurate 6mA site prediction. Traditional machine learning methods, on the other hand, rely heavily on manual features and integrated learning to improve performance, resulting in a reliance on prior knowledge and a large model scale. Furthermore, many models are only trained and tested for one species, with no comparison of model generalization performance, resulting in models with limited practical usability. In order to increase the generalization capability of the model, we propose a lightweight structure predictor Plant6mA based on Transformer encoder. Based on results on independent test sets, our proposed Plant6mA has better generalization performance than the most advanced methods in predicting 6mA location in plant genomes. Plant6mAs MultiHead Attention mechanism effectively enhances its expressive ability by capturing potential biol. information from multiple scales of the input sequence. Furthermore, we used a dimensionality reduction tool to visualize Plant6mAs training process and visually demonstrate the effectiveness of our model. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1

The Article related to plant6ma plant genome n6 methyladenine lightweight structure, bioinformatics, dna methylation, deep learning, Plant Biochemistry: Classical Genetics and Phylogeny and other aspects.HPLC of Formula: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2021, Rahman, Chowdhury Rafeed; Amin, Ruhul; Shatabda, Swakkhar; Toaha, Sadrul Islam Md. published an article.Related Products of 443-72-1 The title of the article was A convolution based computational approach towards DNA N6-methyladenine site identification and motif extraction in rice genome. And the article contained the following:

DNA N6-methylation (6mA) in Adenine nucleotide is a post replication modification responsible for many biol. functions. Automated and accurate computational methods can help to identify 6mA sites in long genomes saving significant time and money. Our study develops a convolutional neural network (CNN) based tool i6mA-CNN capable of identifying 6mA sites in the rice genome. Our model coordinates among multiple types of features such as PseAAC (Pseudo Amino Acid Composition) inspired customized feature vector, multiple one hot representations and dinucleotide physicochem. properties. It achieves auROC (area under Receiver Operating Characteristic curve) score of 0.98 with an overall accuracy of 93.97% using fivefold cross validation on benchmark dataset. Finally, we evaluate our model on three other plant genome 6mA site identification test datasets. Results suggest that our proposed tool is able to generalize its ability of 6mA site identification on plant genomes irresp. of plant species. An algorithm for potential motif extraction and a feature importance anal. procedure are two by products of this research. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Related Products of 443-72-1

The Article related to dna nmethyladenine motif extraction rice genome computational method, Plant Biochemistry: Classical Genetics and Phylogeny and other aspects.Related Products of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 1981, Lopyrev, V. A.; Larina, L. I.; Vakul’skaya, T. I.; Larin, M. F.; Nefedova, O. B.; Shibanova E. F.; Voronkov, M. G. published an article.Recommanded Product: 5709-67-1 The title of the article was Transmission of the substituent effects in 2-substituted benzimidazoles studied by proton and carbon-13 nuclear magnetic resonance. And the article contained the following:

Substituent effects on the 1H and 13C NMR chem. shifts in 2-substituted benzimidazoles and their anions and cations were studied quant. The transmission of electron effects of substituents from C-2 to C-5(6) is approx. 20% less effective than in the opposite direction. The solvent effects on 1H chem. shifts and transmission effects in the charged forms of 2-substituted benzimidazoles were also studied. The experimental process involved the reaction of 2-Nitro-1H-benzo[d]imidazole(cas: 5709-67-1).Recommanded Product: 5709-67-1

The Article related to benzimidazole nmr substituent effect, transmission electronic effect benzimidazole substituent, solvent effect benzimidazole nmr, lfer benzimidazole nmr, Physical Organic Chemistry: Spectral and Related Studies and other aspects.Recommanded Product: 5709-67-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

War, Javeed Ahmad; Srivastava, Santosh Kumar published an article in 2020, the title of the article was Rationale design and synthesis of some novel imidazole linked thiazolidinone hybrid molecules as DNA minor groove binders.Application In Synthesis of N-(3-Aminopropyl)-imidazole And the article contains the following content:

A new series of imidazole linked thiazolidinone hybrid mols. was designed and subsequently synthesized through a feasible, three step reaction protocol. The structures of these mols. were established using FT-IR, 1H NMR, 13C NMR and HRMS techniques. In vitro susceptibility tests against some Gram pos. (Staphylococcus aureus and Bacillus subtilis) and Gram neg. bacteria (Escherichia coli and Pseudomonas aeruginosa) exhibited broad spectrum potency of the mols. The most potent mol. (S2A7) amongst the screened mols., showed min. inhibitory concentration (MIC) value not less than 2.0μg/mL which was at par with the reference drug Streptomycin. Structure activity relationships revealed nitro and chloro groups being crucial for bioactivity when present at meta position of arylidene ring in 3-(3-(imidazol-1-yl)propyl)-5-(benzylidene)-2- (phenylimino)thiazolidin-4-one. DNA (DNA)and bovine serum albumin (BSA) binding studies for S2A7 under simulated physiol. pH were probed using UV Visible, fluorescence quenching, gel electrophoresis and mol. docking techniques. These studies established that S2A7 has strong binding affinity towards DNA and binds at the minor groove of DNA with binding constant (Kb) of 0.1287×102 L/mol. Mol. docking simulations of S2A7 with DNA and BSA predicted binding affinity of -9.2 and -7.2 kcal/mol, resp. Van der Waals forces and hydrogen bonding interactions were predicted as the main forces of interaction. With DNA, S2A7 exhibited specific binding affinity towards adenine-thiamine base pairs. The compound S2A7 forms a stable complex with BSA by binding at subdomain IIIA implying high bio-distribution of the compound The experimental process involved the reaction of N-(3-Aminopropyl)-imidazole(cas: 5036-48-6).Application In Synthesis of N-(3-Aminopropyl)-imidazole

The Article related to imidazole thiazolidinone antibiotic dna minor groove binder bacterial infection, Pharmacology: Drug Interactions and General Pharmacology and other aspects.Application In Synthesis of N-(3-Aminopropyl)-imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Douvlataniotis, Karolos; Bensberg, Maike; Lentini, Antonio; Gylemo, Bjoern; Nestor, Colm E. published an article in 2020, the title of the article was No evidence for DNA N6 -methyladenine in mammals.HPLC of Formula: 443-72-1 And the article contains the following content:

N6 -methyladenine (6mdA) is a widespread DNA modification in bacteria. More recently, 6mdA has also been characterized in mammalian DNA. However, measurements of 6mdA abundance and profiles are often very dissimilar between studies, even when performed on DNA from identical mammalian cell types. Using comprehensive bioinformatics analyses of published data and novel exptl. approaches, we reveal that efforts to assay 6mdA in mammals have been severely compromised by bacterial contamination, RNA contamination, technol. limitations, and antibody nonspecificity. These complications render 6mdA an exceptionally problematic DNA modification to study and have resulted in erroneous detection of 6mdA in several mammalian systems. Together, our results strongly imply that the evidence published to date is not sufficient to support the presence of 6mdA in mammals. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1

The Article related to dna n methyladenine mammalian cell, Mammalian Biochemistry: Classical Genetics and Phylogeny and other aspects.HPLC of Formula: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Kost, A. A.; Ermolin, S. V. published an article in 1978, the title of the article was Fluorescent derivatives of cytosine.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one And the article contains the following content:

The fluorescence of ethenocytosines, e.g., I (R = Me, H, Ph; R1 = H, Me), was investigated by PPP MO calculations The π-electronic charges of the atoms do not change appreciably on excitation of the mols. The electronic excitation of these compounds is more diffuse than that of cytosine. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one

The Article related to fluorescent ethenocytosine mo, Physical Organic Chemistry: Spectral and Related Studies and other aspects.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 1979, Larina, L. I.; Vakul’skaya, T. I.; Nefedova, O. B.; Shibanova, E. F.; Lopyrev, V. A.; Voronkov, M. G. published an article.Reference of 2-Nitro-1H-benzo[d]imidazole The title of the article was Study of electron effects of substituents in 2-substituted benzimidazole derivatives by a proton NMR method. And the article contained the following:

NMR chem. shifts (δ) were determined for the protons on C-4 and C-7 and on C-5 and C-6 of 2-substituted benzimidazoles and their anions and protonated forms. In the neutral mols. substituent effects were transmitted to positions 4 and 7 exclusively by conjugation but were transmitted to positions 5 and 6 by both field and resonance mechanisms in a 1:3 ratio. In the anions transmission to positions 5 and 6 involved only conjugation, whereas transmission to positions 4 and 7 involved inductive and resonance effects in a 1:8 ratio. The effects in the protonated forms could not be separated The experimental process involved the reaction of 2-Nitro-1H-benzo[d]imidazole(cas: 5709-67-1).Reference of 2-Nitro-1H-benzo[d]imidazole

The Article related to nmr benzimidazole lfer, Physical Organic Chemistry: Spectral and Related Studies and other aspects.Reference of 2-Nitro-1H-benzo[d]imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 15, 2021, Diao, Li-Ting; Xie, Shu-Juan; Yu, Pei-Jie; Sun, Yu-Jia; Yang, Fan; Tan, Ye-Ya; Tao, Shuang; Hou, Ya-Rui; Zheng, Ling-Ling; Xiao, Zhen-Dong; Zhang, Qi published an article.Formula: C6H7N5 The title of the article was N6-methyladenine demethylase ALKBH1 inhibits the differentiation of skeletal muscle. And the article contained the following:

DNA N6-methyladenine (N6-mA) was recently recognized as a new epigenetic modification in mammalian genome, and ALKBH1 was discovered as its demethylase. Knock-out mice studies revealed that ALKBH1 was indispensable for normal embryonic development. However, the function of ALKBH1 in myogenesis is largely unknown. In this study, we found that N6-mA showed a steady increase, going along with a strong decrease of ALKBH1 during skeletal muscle development. Our results also showed that ALKBH1 enhanced proliferation and inhibited differentiation of C2C12 cells. Genome-wide transcriptome anal. and reporter assays further revealed that ALKBH1 accomplished the differentiation inhibiting function by regulating a core set of genes and multiple signaling pathways, including increasing chemokine (C-X-C motif) ligand 14 (CXCL14) and activating ERK signaling. Taken together, our results demonstrated that ALKBH1 is critical for the myogenic differentiation of C2C12 cells, and suggested that N6-mA might be a new epigenetic mechanism for the regulation of myogenesis. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to n6methyladenine dna methylation alkbh1 skeletal muscle differentiation, alkbh1, n(6)-methyladenine, rna-seq, skeletal muscle differentiation, Mammalian Biochemistry: General Physiological Chemistry and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On February 1, 1994, Dosanjh, M. K.; Chenna, A.; Kim, E.; Fraenkel-Conrat, H.; Samson, L.; Singer, B. published an article.Application In Synthesis of Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was All four known cyclic adducts formed in DNA by the vinyl chloride metabolite chloroacetaldehyde are released by a human DNA glycosylase. And the article contained the following:

The authors have previously reported that human cells and tissues contain a 1,N6-ethenoadenine (εA)-binding protein, which, through glycosylase activity, releases both 3-methyladenine (m3A) and εA from DNA treated with methylating agents or the vinyl chloride metabolite chloroacetaldehyde, resp. The authors now find that both the partially purified human εA-binding protein and cell-free extracts containing the cloned human m3A-DNA glycosylase release all 4 cyclic etheno adducts – namely εA, 3,N4-ethenocytosine (εC), N2,3-ethenoguanine (N2,3-εG), and 1,N2-ethenoguanine (1,N2-εG). Base release was both time- and protein concentration-dependent. Both εA and εC were excised at similar rates, while 1,N2-εG and N2,3-εG were released much more slowly under identical conditions. The cleavage of glycosyl bonds of several heterocyclic adducts as well as those of simple methylated adducts by the same human glycosylase appears unusual in enzymol. This raises the question of how such a multiple, divergent activity evolved in humans and what may be its primary substrate. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Application In Synthesis of Imidazo[1,2-c]pyrimidin-5(6H)-one

The Article related to chloroacetaldehyde cyclic etheno adduct release, methyladenine dna glycosylase human chloroacetaldehyde, Toxicology: Chemicals (Household, Industrial, General) and other aspects.Application In Synthesis of Imidazo[1,2-c]pyrimidin-5(6H)-one

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem