Tag: 100-200

On September 23, 2021, Kim, Young Kwan; Jo, Min Mi; Park, Jun published a patent.Safety of (1-Ethyl-1H-imidazol-5-yl)methanamine dihydrochloride The title of the patent was Preparation of GLP-1 receptor agonist for treatment or prophylaxis of various metabolic diseases. And the patent contained the following:

The present invention relates to a novel compound I [A = (CH2)m, O, N(Ra) where m = 1-3; Ra = hydrogen, alkyl; R1 = (cycloalkyl)alkyl, (heterocycloalkyl)alkyl, arylalkyl, heteroarylalkyl; R2-R4 = hydrogen, deuterium, halogen, etc.; n = 1-4; Z1-Z7 = CH, CF, CCl, CBr, CI, N; isomer or pharmaceutically acceptable salt thereof] useful as an agent for treatment or prophylaxis of various metabolic diseases such as obesity or diabetes and hyperlipidemia, by means of excellent GLP-1 agonist activity and an excellent DMPK profile. For example II was prepared and exhibited EC50 of 0.007 nM against GLP-1R. The experimental process involved the reaction of (1-Ethyl-1H-imidazol-5-yl)methanamine dihydrochloride(cas: 1255717-13-5).Safety of (1-Ethyl-1H-imidazol-5-yl)methanamine dihydrochloride

The Article related to preparation glp 1 receptor agonist treatment prophylaxis metabolic disease, Heterocyclic Compounds (More Than One Hetero Atom): Imidazoles and other aspects.Safety of (1-Ethyl-1H-imidazol-5-yl)methanamine dihydrochloride

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On October 29, 2014, Duan, Hongdong; Xu, Ting; Bu, Juan published a patent.Application In Synthesis of 2-Nitro-1H-benzo[d]imidazole The title of the patent was Benzimidazolylbenzaldehyde p-phenylenediamine Schiff base, its preparation method and application in fluorescence material and sensor. And the patent contained the following:

The invention disclosed a kind of benzimidazolylbenzaldehyde p-phenylenediamine Schiff base, its preparation method and application in fluorescence material and sensor. The claimed benzimidazolylbenzaldehyde p-phenylenediamine Schiff base is shown in structure I (R = H, F, Cl, NO2, OH, CN). The claimed compound is prepared with benzimidazole or benzimidazole derivative and p-fluorobenzaldehyde via N-C coupling to obtain 4-benzimidazolylbenzaldehyd or its derivative, then condensation with p-phenylenediamine to generate the Schiff base product. The obtained compound has the advantages of conjugated and rigid plane structure with good fluorescence performance and metal ion selectivity. The experimental process involved the reaction of 2-Nitro-1H-benzo[d]imidazole(cas: 5709-67-1).Application In Synthesis of 2-Nitro-1H-benzo[d]imidazole

The Article related to benzimidazolylbenzaldehyde phenylenediamine schiff base preparation, Heterocyclic Compounds (More Than One Hetero Atom): Imidazoles and other aspects.Application In Synthesis of 2-Nitro-1H-benzo[d]imidazole

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Stensio, Karl E.; Wahlberg, Kerstin; Wahren, Robert published an article in 1973, the title of the article was Synthesis of brominated imidazoles.COA of Formula: C4H3BrN2O And the article contains the following content:

Bromination of imidazole in HOAc containing NaOAc gives 2,4,5-tribromoimidazole (I) in a good yield. 4(5)-(2-Chloroethyl)imidazole, imidazole-4(5)-carboxaldehyde, and 4(5)-hydroxymethylimidazole were similarly brominated. Debromination of I with Na2SO3 or BuLi gives 4(5)-bromoimidazole. A simple synthesis of 4(5)-(2-bromoethyl)imidazole is described. The experimental process involved the reaction of 5-Bromo-1H-imidazole-4-carbaldehyde(cas: 50743-01-6).COA of Formula: C4H3BrN2O

The Article related to bromination imidazole, Heterocyclic Compounds (More Than One Hetero Atom): Imidazoles and other aspects.COA of Formula: C4H3BrN2O

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2021, Joshi, Suraj; Ujaoney, Aman Kumar; Ghosh, Payel; Deobagkar, Deepti D.; Basu, Bhakti published an article.Name: N-Methyl-7H-purin-6-amine The title of the article was N6-methyladenine and epigenetic immunity of Deinococcus radiodurans. And the article contained the following:

DNA methylation is ubiquitously found in all three domains of life. This epigenetic modification on adenine or cytosine residues serves to regulate gene expression or to defend against invading DNA in bacteria. Here, we report the significance of N6-methyladenine (6mA) to epigenetic immunity in Deinococcus radiodurans. Putative protein encoded by DR_2267 ORF (Dam2DR) contributed 35% of genomic 6mA in D. radiodurans but did not influence gene expression or radiation resistance. Dam2DR was characterized to be a functional S-adenosyl methionine (SAM)-dependent N6-adenine DNA methyltransferase (MTase) but with no endonuclease activity. Adenine methylation from Dam2DR or Dam1DR (N6-adenine MTase encoded by DR_0643) improved DNA uptake during natural transformation. To the contrary, methylation from Escherichia coli N6-adenine MTase (DamEC that methylates adenine in GATC sequence) on donor plasmid drastically reduced DNA uptake in D. radiodurans, even in presence of Dam2DR or Dam1DR methylated adenines. With these results, we conclude that self-type N6-adenine methylation on donor DNA had a protective effect in absence of addnl. foreign methylation, a sep. methylation-dependent Restriction Modification (R-M) system effectively identifies and limits uptake of G6mATC sequence containing donor DNA. This is the first report demonstrating presence of epigenetic immunity in D. radiodurans. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to deinococcus n6 methyladenine epigenetic immunity, dna uptake, dr_2267, deinococcus radiodurans, n6-methyladenine, restriction modification, transformation efficiency, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Yang, Xiao; Ye, Xiucai; Li, Xuehong; Wei, Lesong published an article in 2021, the title of the article was iDNA-MT: identification DNA modification sites in multiple species by using multi-task learning based a neural network tool.Quality Control of N-Methyl-7H-purin-6-amine And the article contains the following content:

DNA N4-methylcytosine (4mC) and N6-methyladenine (6mA) are two important DNA modifications and play crucial roles in a variety of biol. processes. Accurate identification of the modifications is essential to better understand their biol. functions and mechanisms. However, existing methods to identify 4mA or 6mC sites are all single tasks, which demonstrates that they can identify only a certain modification in one species. Therefore, it is desirable to develop a novel computational method to identify the modification sites in multiple species simultaneously. In this study, we proposed a computational method, called iDNA-MT, to identify 4mC sites and 6mA sites in multiple species, resp. The proposed iDNA-MT mainly employed multi-task learning coupled with the bidirectional gated recurrent units (BGRU) to capture the sharing information among different species directly from DNA primary sequences. Exptl. comparative results on two benchmark datasets, containing different species resp., show that either for identifying 4mA or for 6mC site in multiple species, the proposed iDNA-MT outperforms other state-of-the- art single-task methods. The promising results have demonstrated that iDNA-MT has great potential to be a powerful and practically useful tool to accurately identify DNA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Quality Control of N-Methyl-7H-purin-6-amine

The Article related to dna modification site multi task learning neural network tool, dna modification, deep learning, feature representation, multi-task learning, neural network, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Quality Control of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2021, Chen, Zheng; Liu, Yan; Wang, Hailin published an article.Application of 443-72-1 The title of the article was Conjoint expression and purification strategy for acquiring proteins with ultra-low DNA N6-methyladenine backgrounds in Escherichia coli. And the article contained the following:

DNA N6-methyladenine (6mA), a kind of DNA epigenetic modification, is widespread in eukaryotes and prokaryotes. An enzyme activity study coupled with 6mA detection using ultra-high-performance liquid chromatog.-quadruple mass spectrometry (UHPLC-MS/MS) is commonly applied to investigate 6mA potentially related enzymes in vitro. However, the protein expressed in a common Escherichia coli (E. coli) strain shows an extremely high 6mA background due to minute co-purified bacterial DNA, though it has been purified to remove DNA using multiple strategies. Furthermore, as occupied by DNA with abundant 6mA, the activity of 6mA-related proteins will be influenced seriously. Here, to address this issue, we for the first time construct a derivative of E. coli Rosetta (DE3) via the λRed knockout system specifically for the expression of 6mA-related enzymes. The gene dam encoding the 6mA methyltransferase (MTase) is knocked out in the newly constructed strain named LAMBS (low adenine methylation background strain). Contrasting with E. coli Rosetta (DE3), LAMBS shows an ultra-low 6mA background on the genomic DNA when analyzed by UHPLC-MS/MS. We also demonstrate an integral strategy of protein purification, coupled with the application of LAMBS. As a result, the purified protein expressed in LAMBS exhibits an ultra-low 6mA background comparing with the one expressed in E. coli Rosetta (DE3). Our integral strategy of protein expression and purification will benefit the in vitro investigation and application of 6mA-related proteins from eukaryotes, although these proteins are elusive until now. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to escherichia dna n6 methyladenine protein expression purification, dna n6-methyladenine, e. coli rosetta (de3), uhplc-ms/ms, low background, λred system, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2021, Chaturvedi, Anurag; Cruz Corella, Joaquim; Robbins, Chanz; Loha, Anita; Menin, Laure; Gasilova, Natalia; Masclaux, Frederic G.; Lee, Soon-Jae; Sanders, Ian R. published an article.Formula: C6H7N5 The title of the article was The methylome of the model arbuscular mycorrhizal fungus, Rhizophagus irregularis, shares characteristics with early diverging fungi and Dikarya. And the article contained the following:

Early-diverging fungi (EDF) are distinct from Dikarya and other eukaryotes, exhibiting high N6-methyldeoxyadenine (6mA) contents, rather than 5-methylcytosine (5mC). As plants transitioned to land the EDF sub-phylum, arbuscular mycorrhizal fungi (AMF; Glomeromycotina) evolved a symbiotic lifestyle with 80% of plant species worldwide. Here we show that these fungi exhibit 5mC and 6mA methylation characteristics that jointly set them apart from other fungi. The model AMF, R. irregularis, evolved very high levels of 5mC and greatly reduced levels of 6mA. However, unlike the Dikarya, 6mA in AMF occurs at sym. ApT motifs in genes and is associated with their transcription. 6MA is heterogeneously distributed among nuclei in these coenocytic fungi suggesting functional differences among nuclei. While far fewer genes are regulated by 6mA in the AMF genome than in EDF, most strikingly, 6mA methylation has been specifically retained in genes implicated in components of phosphate regulation; the quintessential hallmark defining this globally important symbiosis. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to rhizophagus irregularis arbuscular mycorrhizal fungus characteristic methyldeoxyadenine methylcytosine, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2020, Wang, Hao-Tian; Xiao, Fu-Hui; Li, Gong-Hua; Kong, Qing-Peng published an article.Reference of N-Methyl-7H-purin-6-amine The title of the article was Identification of DNA N6-methyladenine sites by integration of sequence features. And the article contained the following:

Abstract: Background: An increasing number of nucleic acid modifications have been profiled with the development of sequencing technologies. DNA N6-methyladenine (6mA), which is a prevalent epigenetic modification, plays important roles in a series of biol. processes. So far, identification of DNA 6mA relies primarily on time-consuming and expensive exptl. approaches. However, in silico methods can be implemented to conduct preliminary screening to save exptl. resources and time, especially given the rapid accumulation of sequencing data. Results: In this study, we constructed a 6mA predictor, p6mA, from a series of sequence-based features, including physicochem. properties, position-specific triple-nucleotide propensity (PSTNP), and electron-ion interaction pseudopotential (EIIP). We performed maximum relevance maximum distance (MRMD) anal. to select key features and used the Extreme Gradient Boosting (XGBoost) algorithm to build our predictor. Results demonstrated that p6mA outperformed other existing predictors using different datasets. Conclusions: p6mA can predict the methylation status of DNA adenines, using only sequence files. It may be used as a tool to help the study of 6mA distribution pattern. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine

The Article related to dna nitrogen methyladenine algorimthm, dna n6-methyladenine, machine learning, xgboost, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Reference of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2020, Li, Jianwei; Huang, Yan; Cui, Qinghua; Zhou, Yuan published an article.Reference of N-Methyl-7H-purin-6-amine The title of the article was m6Acorr: an online tool for the correction and comparison of m6A methylation profiles. And the article contained the following:

The anal. and comparison of RNA m6A methylation profiles have become increasingly important for understanding the post-transcriptional regulations of gene expression. However, current m6A profiles in public databases are not readily intercomparable, where heterogeneous profiles from the same exptl. report but different cell types showed unwanted high correlations. Several normalizing or correcting methods were tested to remove such laboratory bias. And m6Acorr, an effective pipeline for correcting m6A profiles, was presented on the basis of quantile normalization and empirical Bayes batch regression method. m6Acorr could efficiently correct laboratory bias in the simulated dataset and real m6A profiles in public databases. The preservation of biol. signals was examined after correction, and m6Acorr was found to better preserve differential methylation signals, m6A regulated targets, and m6A-related biol. features than alternative methods. Finally, the m6Acorr server was established. This server could eliminate the potential laboratory bias in m6A methylation profiles and perform profile-profile comparisons and functional anal. of hyper- (hypo-) methylated genes based on corrected methylation profiles. M6Acorr was established to correct the existing laboratory bias in RNA m6A methylation profiles and perform profile comparisons on the corrected datasets. The m6Acorr server is available at http://www.rnanut.net/m6Acorr. A stand-alone version with the correction function is also available in GitHub at https://github.com/emersON106/m6Acorr. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine

The Article related to m6acorr methylation profile mettl3 mettl14, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Reference of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Liu, Chang; Li, Linshuang; Yang, Bo; Zhao, Yiqing; Dong, Xiyuan; Zhu, Lixia; Ren, Xinling; Huang, Bo; Yue, Jing; Jin, Lei; Zhang, Hanwang; Wang, Lan published an article.Application of 443-72-1 The title of the article was Transcriptome-wide N6-methyladenine methylation in granulosa cells of women with decreased ovarian reserve. And the article contained the following:

The emerging epitranscriptome plays an essential role in female fertility. As the most prevalent internal mRNA modification, N6-methyladenine (m6A) methylation regulate mRNA fate and translational efficiency. However, whether m6A methylation was involved in the aging-related ovarian reserve decline has not been investigated. Herein, we performed m6A transcriptome-wide profiling in the ovarian granulosa cells of younger women (younger group) and older women (older group). M6A methylation distribution was highly conserved and enriched in the CDS and 3’UTR region. Besides, an increased number of m6A methylated genes were identified in the older group. Bioinformatics anal. indicated that m6A methylated genes were enriched in the FoxO signaling pathway, adherens junction, and regulation of actin cytoskeleton. A total of 435 genes were differently expressed in the older group, moreover, 58 of them were modified by m6A. Several specific genes, including BUB1B, PHC2, TOP2A, DDR2, KLF13, and RYR2 which were differently expressed and modified by m6A, were validated using qRT-PCR and might be involved in the decreased ovarian functions in the aging ovary. Hence, our finding revealed the transcriptional significance of m6A modifications and provide potential therapeutic targets to promote fertility reservation for aging women. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to human ovarian reserve methyladenine methylation transcriptome granulosa cell, aging, granulosa cell, n6-methyladenosine, ovary, rna modification, Mammalian Pathological Biochemistry: Obstetrics – Gynecology and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem