Tag: M.W=100-150

Liu, Xuerui; Dong, Lina; Wang, Lianxiao; Xu, Hui; Gao, Shanmin; Zhong, Linlin; Zhang, Shengxiao; Jiang, Tingting published the artcile< 2-Aminopurine modified DNA probe for rapid and sensitive detection of L-cysteine>, Computed Properties of 452-06-2, the main research area is cysteine biosensor ssDNA mercury aminopurine fluorescence water milk serum; 2-Aminopurine; Exonuclease I; Fluorescence method; L-cysteine; Thymine-thymine mismatches.

A rapid, cost-effective and quencher-free fluorescence-based anal. method for sensitive detection of L-cysteine (Cys) based on 2-aminopurine (2-AP) labeled DNA probe and exonuclease I (Exo I) activity was developed. 2-AP labeled DNA probe includes two thymine (T)-T mismatches, which can bind with Hg2+ to form T-Hg2+-T pairing bases, resulting in stable hairpin with five base pairs in its stem. The target Cys can remove Hg2+ from the stem of the hairpin probe based on the high affinity of Cys with Hg2+, leading to the unfolding of the hairpin probe. At last, by adding Exo I, the resulted single-stranded DNA (ssDNA) will be digested to release free 2-AP with strong fluorescence. Under the optimal conditions, the sensing system exhibited a good and wider linear range from 0.4 to 400 nM (R2 = 0.997) and a detection limit as low as 0.16 nM for Cys. Furthermore, other amino acids without reductive sulfur group did not generate obvious change in fluorescence signals. Finally, the sensor can be used in diluted real samples with a good recovery rate, showing promising application in food, environmental and medical anal.

Talanta published new progress about Beverages. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Computed Properties of 452-06-2.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Zhao, Xiaojia; Zhu, Zicheng; Zou, Rong; Wang, Lingyun; Gong, Hang; Cai, Changqun published the artcile< An enzyme-free three-dimensional DNA walker powered by catalytic hairpin assembly for H5N1 DNA ratiometric detection>, Computed Properties of 452-06-2, the main research area is DNA walker catalytic hairpin assembly ratiometric detection.

The reliable and quant. detection of virus DNA is highly desirable for the early diagnosis and clin. treatment of diseases. In this work, a three-dimensional (3D) DNA walker based on a non-enzyme-mediated nucleic acid cascade amplification reaction was constructed for the ratiometric detection of H5N1 DNA. When the DNA walker was developed by the surface modification of aminated silica microsphere (SiO2-NH2) with H1 hairpin structures were activated in the presence of target DNA, the fluorescence of the H1-conjugated Thioflavin T (ThT) fluorophore (495 nm) was turned on, while the fluorescence of 2-aminopurine (2-AP) (363 nm) conjugated to the H2 hairpin remained unchanged. This strategy combines DNA walker powered by catalyzed hairpin assembly (CHA) reaction with proportional fluorescence signal output methods, which can effectively reduce the risk of generating false-pos. signals. The developed DNA walker demonstrated excellent anal. sensitivity and specificity, with a detection limit of 60 pM. The DNA walker was also capable of selectively detecting H5N1 DNA in clin. blood serum samples, which demonstrated its potential for use in various clin. applications.

Microchemical Journal published new progress about Blood serum. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Computed Properties of 452-06-2.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Dong, Tingting; Sha, Yueqi; Liu, Hairong; Sun, Liwei published the artcile< Altitudinal Variation of Metabolites, Mineral Elements and Antioxidant Activities of Rhodiola crenulata (Hook.f. and Thomson) H.Ohba>, Recommanded Product: 7H-Purin-2-amine, the main research area is Rhodiola crenulata metabolite mineral element antioxidant activity; change; element; high altitude; metabolite; rhodiola.

Rhodiolacrenulata (Hook.f. & Thomson) H.Ohba is an alpine medicinal plant that can survive in extreme high altitude environments. However, its changes to extreme high altitude are not yet clear. In this study, the response of Rhodiola crenulata to differences in altitude gradients was investigated through chem., ICP-MS and metabolomic methods. A targeted study of Rhodiola crenulata growing at three vertical altitudes revealed that the contents of seven elements Ca, Sr, B, Mn, Ni, Cu, and Cd, the phenolic components, the ascorbic acid, the ascorbic acid/dehydroascorbate ratio, and the antioxidant capacity were pos. correlated with altitude, while the opposite was true for total ascorbic acid content. Furthermore, 1165 metabolites were identified: flavonoids (200), gallic acids (30), phenylpropanoids (237), amino acids (100), free fatty acids and glycerides (56), nucleotides (60), as well as other metabolites (482). The differential metabolite and biomarker analyses suggested that, with an increasing altitude: (1) the shikimic acid-phenylalanine-phenylpropanoids-flavonoids pathway was enhanced, with phenylpropanoids upregulating biomarkers much more than flavonoids; phenylpropanes and phenylmethanes upregulated, and phenylethanes downregulated; the upregulation of quercetin was especially significant in flavonoids; upregulation of condensed tannins and downregulation of hydrolyzed tannins; upregulation of shikimic acids and amino acids including phenylalanine. (2) significant upregulation of free fatty acids and downregulation of glycerides; and (3) upregulation of adenosine phosphates. Our findings provide new insights on the responses of Rhodiola crenulata to extreme high altitude adversity.

Molecules published new progress about Amino acids Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Recommanded Product: 7H-Purin-2-amine.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Sun, Mengshi; Wu, Siting; Kang, Shaozhu; Liao, Jiaming; Zhang, Luhao; Xu, Zhuqing; Chen, Hong; Xu, Linting; Zhang, Xin; Qin, Qiwei; Wei, Jingguang published the artcile< Critical roles of G3BP1 in red-spotted grouper nervous necrosis virus-induced stress granule formation and viral replication in orange-spotted grouper (Epinephelus coioides)>, Quality Control of 452-06-2, the main research area is Epinephelus coioides G3BP1 nervous necrosis virus viral replication; Epinephelus coioides; G3BP1; RGNNV; SGs; virus replication.

Viral infection causes changes in the internal environment of host cells, and a series of stress responses are generated to respond to these changes and help the cell survive. Stress granule (SG) formation is a type of cellular stress response that inhibits viral replication. However, the relationship between red-spotted grouper nervous necrosis virus (RGNNV) infection and SGs, and the roles of the SG marker protein RAS GTPase-activating protein (SH3 domain)-binding protein 1 (G3BP1) in viral infection remain unclear. In this study, RGNNV infection induced grouper spleen (GS) cells to produce SGs. The SGs particles co-located with the classic SG marker protein eIF3η, and some SGs depolymerized under treatment with the translation inhibitor, cycloheximide (CHX). In addition, when the four kinases of the eukaryotic translation initiation factor 2α (eIF2α)-dependent pathway were inhibited, knockdown of HRI and GCN2 with small interfering RNAs and inhibition of PKR with 2-aminopurine had little effect on the formation of SGs, but the PERK inhibitor significantly inhibited the formation of SGs and decreased the phosphorylation of eIF2α. G3BP1 of Epinephelus coioides (named as EcG3BP1) encodes 495 amino acids with a predicted mol. weight of 54.12 kDa and 65.9% homol. with humans. Overexpression of EcG3BP1 inhibited the replication of RGNNV in vitro by up-regulating the interferon and inflammatory response, whereas knockdown of EcG3BP1 promoted the replication of RGNNV. These results provide a better understanding of the relationship between SGs and viral infection in fish.

Frontiers in Immunology published new progress about Amino acids Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Quality Control of 452-06-2.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Zhao, Han; Chen, Mingjian; Ma, Changbei published the artcile< Fluorescent method for the detection of biothiols using an Ag+-mediated conformational switch>, COA of Formula: C5H5N5, the main research area is silver glutathione cysteine biothiol fluorescence conformational switch; 2-aminopurine; cysteine; fluorescence; glutathione.

In this work, a novel, simple, and time-saving fluorescence approach for the detection of biothiols (glutathione and cysteine) was developed by employing a DNA probe labeled with 2-aminopurine. As an adenine analog, 2-aminopurine exhibits high fluorescence intensity that can be rapidly quenched in the presence of DNA. In the presence of Ag+, the fluorescence increased significantly, which was a result of the formation of cytosine-Ag+-cytosine base pairs and the release of 2-aminopurine. Upon addition of either glutathione or cysteine, the structure of cytosine-Ag+-cytosine was disrupted, a product of the stronger affinity between biothiols and Ag+. As a result, the 2-aminopurine-labeled DNA probe returned to its former structure, and the fluorescence signal was quenched accordingly. The detection limit for glutathione and cysteine was 3 nM and 5 nM, resp. Furthermore, the determination of biothiols in human blood serum provided a potential application for the probe as a diagnostic tool in clin. practice.

Sensors published new progress about Blood serum. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, COA of Formula: C5H5N5.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Li, Qian; Liu, Shenbin; Zhu, Xiaocang; Mi, Wenli; Maoying, Qiliang; Wang, Jun; Yu, Jin; Wang, Yanqing published the artcile< Hippocampal PKR/NLRP1 Inflammasome Pathway Is Required for the Depression-Like Behaviors in Rats with Neuropathic Pain>, Electric Literature of 452-06-2, the main research area is depression neuropathic pain IL1beta PKR NLRP1 inflammasome animal behavior; PKR; anxiety; depression; hippocampus; inflammasome; pain.

The chronic neuropathic pain-associated psychiatric disorders have seriously disturbed the quality of patients’ life, such as depression and anxiety. Neuroinflammation in the hippocampus plays an important role in the neuropathic pain-associated depressive and anxiety disorders, but the underlying mechanism has not been thoroughly elucidated to date. The (NLRP)-1 inflammasome, which controls the production of pro-inflammatory cytokines, was broadly involved in the neuroinflammation-related diseases. In the present study, we show that the NLRP1 inflammasome is significantly activated in the hippocampus of rats subjected to the (CCI)-induced neuropathic pain. Inhibiting the product of NLRP1 inflammasome not only attenuated the depression-like behaviors but also suppressed the production of mature IL-1β in the hippocampus of CCI rats. The double-stranded RNA-dependent protein kinase has been recently shown to be a pivotal regulator for the activation of inflammasome. Functional inhibition of PKR suppressed the NLRP1 inflammasome activation and effectively attenuated the CCI-induced depression-like behaviors. These results indicate that the hippocampal PKR/NLRP1 inflammasome pathway play an important role in the development of the depressive behaviors after chronic neuropathic pain. Thus, interrupting this pathway might provide a novel therapeutic strategy for neuropathic pain-associated depressive disorders.

Neuroscience (Amsterdam, Netherlands) published new progress about Allodynia. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Electric Literature of 452-06-2.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Kang, Ying; Li, Yulan; Xu, Ying; Ji, Zhizhong published the artcile< Studies on synthesis of 2-(4-methoxybenzylidene)-5-aminomethylcyclopentanone derivatives and their antiinflammatory activity>, Recommanded Product: 2-(tert-Butyl)-1H-imidazole, the main research area is cyclopentanone methoxybenzylidene antiinflammatory preparation.

The synthesis of 2-(4-methoxybenzylidene)-5-aminomethylcyclopentanone derivatives and their antiinflammatory activities were studied. Ten new derivatives of 2-(4-methoxybenzylidene)-5-aminomethylcyclopentanonee were prepared The structures of these compounds were confirmed by spectral methods and their antiinflammatory activities were examined by carrageenin-induced rat paw edema test. The amino exchange was an elimination-addition reaction.

Journal of Chinese Pharmaceutical Sciences published new progress about Anti-inflammatory agents. 36947-69-0 belongs to class imidazoles-derivatives, and the molecular formula is C7H12N2, Recommanded Product: 2-(tert-Butyl)-1H-imidazole.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Kladova, O. A.; Kuznetsova, A. A.; Barthes, Nicolas P. F.; Michel, Benoit Y.; Burger, Alain; Fedorova, O. S.; Kuznetsov, N. A. published the artcile< New Fluorescent Analogs of Nucleotides Based on 3-Hydroxychromone for Recording Conformational Changes of DNA>, Formula: C5H5N5, the main research area is hydroxychromone fluorescence quenching DNA conformational change.

It has recently been found that derivatives of nucleotides containing a 3-hydroxychromone fluorescent dye can be used as sensitive markers of conformational changes of DNA. In this work, a comparative anal. of two fluorescent nucleotide derivatives-3-hydroxychromone a (3HC) and 3HC-modified uridine (FCU)-was performed during the study of protein-nucleic acid interactions for several human DNA repair enzymes, removing damaged nucleotides: DNA glycosylases AAG, OGG1, UNG2, and MBD4 and AP endonuclease APE1. The changes of fluorescence intensity significantly depended on the nature of neighbor nucleotides and may be opposite in direction for different cases. The FCU residue located in the complementary strand opposite to damaged nucleotide or in the same strand moved by few nucleotides, is very sensitive to processes induced by DNA glycosylases in the course of formation of enzyme-substrate complexes, which include local melting and bending of the DNA chain, as well as eversion of the damaged nucleotide from DNA double helix and insertion of amino acids of the active site into the void.

Russian Journal of Bioorganic Chemistry published new progress about Amino acids Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Formula: C5H5N5.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Sasaki, Shogo; Ma, Yue; Ishizuka, Takumi; Bao, Hong-Liang; Hirokawa, Takatsugu; Xu, Yan; Tera, Masayuki; Nagasawa, Kazuo published the artcile< Linear consecutive hexaoxazoles as G4 ligands inducing chair-type anti-parallel topology of a telomeric G-quadruplex>, Recommanded Product: 7H-Purin-2-amine, the main research area is telomeric G quadruplex linear consecutive hexaoxazole anti parallel topol.

G-quadruplex structures (G4s) in guanine-rich regions of DNA play critical roles in various biol. phenomena, including replication, translation, and gene expression. There are three types of G4 topol., i.e., parallel, anti-parallel, and hybrid, and ligands that selectively interact with or stabilize a specific topol. have been extensively explored to enable studies of topol.-related functions. Here, we describe the synthesis of a new series of G4 ligands based on 6LCOs (6-linear consecutive oxazoles), i.e., L2H2-2M2EA-6LCO (2), L2A2-2M2EAc-6LCO (3), and L2G2-2M2EG-6LCO (4), which bear four aminoalkyl, acetamidealkyl, and guanidinylalkyl side chains, resp. Among them, ligand 2 stabilized telomeric G4 and induced anti-parallel topol. independently of the presence of cations. The anti-parallel topol. induced by 2 was identified as chair-type by means of 19F NMR spectroscopy and fluorescence experiments with 2-aminopurine-labeled DNA.

RSC Advances published new progress about DNA replication. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Recommanded Product: 7H-Purin-2-amine.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Li, Xianming; Wang, Yanying; Tang, Honghu; Yang, Bing; Zhao, Yi; Wu, Peng published the artcile< Evaluation of the sequence-dependent relative activity of APE1 for optimal biosensing design>, HPLC of Formula: 452-06-2, the main research area is biosensor APE1 uracil DNA glycosylase fluorescence; APE1; Biosensor sensign; Key bases; Sequence-dependent realtive activity.

Apurinic/apyrimidinic endonuclease 1 (APE1) can selectively incise the AP site of DNA, thus is universal for various DNA substrates for flexible endonuclease-assisted signal amplification. However, the substrate preference of APE1 has never been systematically investigated. Therefore in this work, the detailed sequence-dependent relative activity of APE1 was determined It turned out that the APE1 activity did vary with the change of the adjacent and opposite bases, and over 10-fold relative activity difference was observed for different sequence combinations. Such difference is appreciable enough to induce evident impact on APE1-involved biosensing. With an APE1 probe designed for cycled signal amplification, the sensitivities followed exactly with the above activity order. Compared with Nb.BbvCl, the sensitivity of the APE1 probe varied between higher and lower than the Nb. BbvCl probe (with varied substrates), demonstrating the importance of the sequence-dependent relative activity of APE1 for optimal biosensor development. Moreover, the above APE1 probe design was harvested and engineered for sensitive biosensing of uracil-DNA glycosylase (UDG). Through theor. anal. of the interaction between APE1 and the substrates, the accuracy of the determined sequence-dependent relative activity of APE1 was partially confirmed.

Biosensors & Bioelectronics published new progress about Biosensors. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, HPLC of Formula: 452-06-2.

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem