Differentiation of 1,4- and 1,5-disubstituted imidazoles was written by Matthews, H. Randall;Rapoport, Henry. And the article was included in Journal of the American Chemical Society in 1973.Safety of 1-Methyl-4-nitroimidazole This article mentions the following:
A method is presented for distinguishing 1,4- and 1,5-disubstituted imidazoles by their proton cross-ring coupling constants Other spectral methods also have been evaluated, as well as several methods which have been reported for differentiating such isomers. Comparison of these methods leads to the conclusion that the measurement of cross-ring coupling constants is the most generally satisfactory and reliable procedure. On this basis, structures are assigned to the carboxymethylhistidines, and the histamine metabolite is established as 1-β-d-ribofuranosyl-4- imidazoleacetic acid. In the experiment, the researchers used many compounds, for example, 1-Methyl-4-nitroimidazole (cas: 3034-41-1Safety of 1-Methyl-4-nitroimidazole).
1-Methyl-4-nitroimidazole (cas: 3034-41-1) belongs to imidazole derivatives. Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar. Imidazole is incorporated into many important biological compounds. The most pervasive is the amino acid histidine, which has an imidazole side-chain. Histidine is present in many proteins and enzymes, e.g. by binding metal cofactors, as seen in hemoglobin.Safety of 1-Methyl-4-nitroimidazole
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem